“…A model aiming at predicting the temporal development of the mechanical properties more precisely should include the functional interactions between the separate PG and collagen components and should take into account the time course of the synthesis of specific matrix components, which is affected by factors like mechanical stimulation, growth factors, the amount of matrix present, nutrient availability and cell proliferation. 5,37,40,43,60 For example, in contrast to the constant matrix synthesis rate assumed here, it has been demonstrated in several studies that GAG synthesis decreased as a function of time, 5,37 while collagen production can also increase in later stages of culture. 37 To provide a clear comparison between the different matrix distribution cases only the initial, linear elastic, stiffness and undeformed permeability were compared.…”