Glycoproteins of Bovine Viral Diarrhoea-Mucosal Disease Virus in Infected Bovine Cells

Abstract

SUMMARYBovine cell cultures infected with bovine viral diarrhoea-mucosal disease (BVD) virus were radiolabelled with L-[3SS]methionine or D-[2-3H]mannose followed by analysis of the labelled polypeptides by radioimmunoprecipitation and polyacrylamide gel electrophoresis in one and two dimensions. Six glycoproteins were detected in infected cells. Two abundant species had Mr of 48K and 56K to 58K while the less abundant species had Mr of 118K, 75K, 65K and 25K. When cells were radiolabelled with L-[35S]methionine in the presence of tunicamycin 56K to 58K migrated with apparent masses of 54K (a minor species) and 48K to 50K (the major molecular species) in PAGE. Endoglycosidase F digestion of virus-induced polypeptides caused a 4K to 6K reduction in the apparent molecular mass of 56K to 58K yielding a single 52K digested product, indicating that theheterogeneity of 56K to 58K was due to differences in the oligosaccharide moieties. Tunicamycin caused a drastic reduction in the yield of infectious virus indicating that the carbohydrate moieties serve a critical role in the infectious cycle of BVD virus.

Immunoaffinity purification and characterization of the envelope protein E1 of hog cholera virus

“…It is obvious that the polypeptide backbone is not always fully glycosylated, but assuming that N-linked glycans have an average M r of 2K to 3K, three to four glycans may be attached to a fully glycosylated protein. The above data agree with those of Donis & Dubovi (1987), who demonstrated that deglyco- Fig. 7.…”

The Amino-Terminal Domain of Bovine Viral Diarrhea Virus NproProtein Is Necessary for Alpha/Beta Interferon Antagonism

“…Bovine testicle cells express epithelial markers and were derived as described previously (18). NCL1 bovine uterine cells are also epithelioid (E. J. Dubovi, unpublished data), whereas CRIB cells resistant to BVDV infection (20) were derived from MDBK cells.…”

Analysis of polypeptides synthesized in bovine respiratory syncytial virus-infected cells

“…Time-course of synthesis of BRSV proteins in BTu cells was determined by pulse labeling infected cell cultures for 30min at 4,8,12,16,20,24,30,36,42,48,60, and 75 h p.i. Uninfected control cells were pulse-labeled under similar conditions.…”