Glycoprotein synthesis and secretion by cultured small intestinal mucosa in coeliac disease.

Crabtree, J. E.; Heatley, R. V.; Losowsky, M. S.

doi:10.1136/gut.30.10.1339

Cited by 16 publications

(18 citation statements)

References 15 publications

(1 reference statement)

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“…As previously demonstrated [8], jejunal glycoprotein synthesis and secretion was higher in untreated coeliac patients (449 ±84.1) than treated patients (275.5 + 52) in the absence of anti CD3 antibody. In treated patients, culture with anti-CD3 antibody resulted in a significant increase (p < 0.05) in glycoprotein synthesis, the mean total incorporation of 3H-glucosamine for anti-CD3 cultures being 469.5 ± 87.3.…”

Section: Effects O F Anti-cd 3 Antibody On Glycoprotein Biosynthesis supporting

confidence: 61%

“…The incorporation of radiolabelled sugars into gas trointestinal glycoproteins is generally considered a good indicator of mucus glycoprotein secretion [12] and correlates with goblet cell density in small intesti nal tissue [8]. However, epithelial brush border mem brane glycoproteins will also be radiolabelled with glucosamine during culture in vitro [15], but in this latter study, the majority of radiolabelled glycopro teins were in high molecular weight fractions with no brush border enzyme activity.…”

Section: Discussionmentioning

confidence: 58%

“…Biopsies were cultured in me dium containing 5 pCi of D-[l-3H]-glucosamine hydrochloride (specific activity 5.8 Ci/mmol; Amersham) for 24 h. At the end of culture, the biopsies and culture medium were processed as previ ously described [8], Control cultures of radiolabelled medium incu bated with no biopsy tissue were similarly processed to correct for non-specific binding of radioactivity to fetal calf serum. Protein determinations of homogenised biopsies (200-pl aliquots) were undertaken using a modified Lowry method [13].…”

Section: Glycoprotein Synthesis and Secretionmentioning

confidence: 99%

“…Jeju nal and duodenal tissue was placed into culture medium consisting of RPMI 1640 containing 23.8 m M sodium bicarbonate (Flow Lab oratories Ltd., Rickmansworth, UK), 40 pg/ml gentamicin and 10% heat-inactivated fetal calf serum (Sera-Lab Ltd., Crawley, UK) and cultured as previously described [8]. Each biopsy was orien tated, mucosal surface upwards, on a sterile stainless steel grid posi tioned over the central well of an organ culture dish (Falcon) containing 1.6 ml of culture medium.…”

Section: Organ Culturementioning

confidence: 99%

“…Immune complexes [2], complement compo nents [3] and macrophage secretory products [4] have all been reported to stimulate epithelial glycoprotein secretion. T lymphocytes have also been implicated in inducing the goblet cell hyperplasia associated with intestinal helminth infections in rats [5,6), and im mune expulsion is accompanied by increased mucus secretion [7], Recently increased intestinal glycoprotein synthe sis and secretion have been demonstrated in patients with untreated coeliac disease [8]; a condition in which there is strong evidence of alterations in muco sal T-cell activation/stimulation [9-11J. To investi gate whether T-cell abnormalities are responsible for the enhanced intestinal glycoprotein production in coeliac disease [8], we have examined the effects of ac tivating endogenous mucosal lymphocytes of normal and coeliac human small intestinal tissue with anti-CD3 antibody or pokeweed mitogen (PWM) on gly coprotein biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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T Lymphocyte Stimulation of Human Small Intestinal Glycoprotein Biosynthesis: Effects of Anti-CD3 Antibody on Normal and Coeliac Mucosa

Crabtree¹,

Heatley²,

Trejdosiewicz³

et al. 1990

Int Arch Allergy Immunol

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The effects of mucosal T-cell activation on human small intestinal glycoprotein biosynthesis have been examined during short-term culture in vitro. The incorporation of ³H-glucosamine into tissue and secreted glycoproteins was determined. Activation of mucosal T lymphocytes of normal duodenal biopsies with the monoclonal anti-CD3 antibody significantly increased both the total glucosamine incorporation into glycoproteins (p < 0.01) and the secretion of in vitro radiolabelled glycoproteins (p < 0.001). This effect was inhibited by ciclosporin. Secretion of glycoproteins was also stimulated by culture with pokeweed mitogen. In patients with coeliac disease, culture of small intestinal biopsies with anti-CD3 antibody significantly increased (p < 0.05) glycoprotein biosynthesis in treated patients, but had no stimulatory effect in untreated patients with villous atrophy. These results show that activation of mucosal T lymphocytes induces quantitative changes in intestinal glycoprotein synthesis and secretion and T lymphocytes therefore have an important role in non-specific intestinal defences. The results are consistent with the suggestion that increased glycoprotein synthesis and secretion in untreated coeliac mucosa result from T-cell activation.

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Section: Effects O F Anti-cd 3 Antibody On Glycoprotein Biosynthesis supporting

confidence: 61%

Section: Discussionmentioning

confidence: 58%

Section: Glycoprotein Synthesis and Secretionmentioning

confidence: 99%

Section: Organ Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

T Lymphocyte Stimulation of Human Small Intestinal Glycoprotein Biosynthesis: Effects of Anti-CD3 Antibody on Normal and Coeliac Mucosa

Crabtree¹,

Heatley²,

Trejdosiewicz³

et al. 1990

Int Arch Allergy Immunol

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Interferon-gamma and Tumour Necrosis Factor Production in Patients withHelicobacter pylori Infection

et al. 1993

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The production of the cytokines, interferon-gamma and tumour necrosis factor by human antral mucosa cells and stimulated peripheral blood mononuclear cells were determined by enzyme linked immunosorbent assay and L929 bioassay respectively. Tumour necrosis factor production by peripheral blood mononuclear cells in response to Helicobacter pylori stimulation was depressed in Helicobacter pylori positive individuals, compared to Helicobacter pylori negative individuals (P < 0.05). There was no difference in tumour necrosis factor production by peripheral blood mononuclear cells in response to purified protein derivative. However, tumour necrosis factor production by cells isolated from gastric mucosa during short term culture was significantly higher in Helicobacter pylori positive patients (P < 0.05) than negative patients, indicating a probable macrophage response. Levels of interferon-gamma did not differ significantly in the gastric explant culture from the two groups. The results show that Helicobacter pylori negative patients have a stronger peripheral cellular immune response to Helicobacter pylori infection. The higher levels of tumour necrosis factor production by antral mucosa cells in Helicobacter pylori positive patients may reflect the infiltration of T lymphocytes and macrophages within the local mucosa.

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MUC2 and MUC6 apomucins expression in human gastric neoplasm: an immunohistochemical analysis

2010

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Apomucins play important biological roles in cell-cell and cell-extracellular matrix interactions, in cell signaling, and in biological properties of cancer cells. Their specific pattern of expression during the different steps of tumor progression toward adenocarcinoma suggests that they play significant roles in tumorigenesis. The family of secreted mucins, gel-forming components of viscoelastic mucus gels protecting the epithelia, includes mucins MUC2 and MUC6. Their principle function is to contribute in mucus formation by forming a tridimensional network via oligomerization domains to protect underlying epithelia against diverse injuries. The current study was investigated the expression of MUC2 and MUC6 in patients with gastric carcinoma. MUC2 and MUC6 expressions were detected immunohistochemically in gastric cancer biopsies using specific monoclonal antibodies. The results showed that in our gastric carcinoma cases, MUC2 expression was detected in 78.6% of cases. MUC2 expression is increased from well differentiated to moderately differentiated to poorly differentiated gastric adenocarcinoma. On the other hand, MUC6 was detected in 32% of cases. The expression of MUC2 is increasing, which is accompanied by an altered expression of MUC6 in gastric cancer. Therefore, it is concluded that the expression pattern of secreted mucins including MUC2 and MUC6 is altered apparently in gastric carcinoma.

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Glycoprotein synthesis and secretion by cultured small intestinal mucosa in coeliac disease.

Cited by 16 publications

References 15 publications

T Lymphocyte Stimulation of Human Small Intestinal Glycoprotein Biosynthesis: Effects of Anti-CD3 Antibody on Normal and Coeliac Mucosa

T Lymphocyte Stimulation of Human Small Intestinal Glycoprotein Biosynthesis: Effects of Anti-CD3 Antibody on Normal and Coeliac Mucosa

Interferon-gamma and Tumour Necrosis Factor Production in Patients withHelicobacter pylori Infection

MUC2 and MUC6 apomucins expression in human gastric neoplasm: an immunohistochemical analysis

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