Glycoprotein non-metastatic melanoma protein b (Gpnmb) is highly expressed in macrophages of acute injured kidney and promotes M2 macrophages polarization

Zhou, Letian; Zhuo, Hui; Ouyang, Huiyu; Liu, Yexin; Fang, Yuan; Sun, Lin; Liu, Fuyou; Liu, Hong

doi:10.1016/j.cellimm.2017.03.006

Cited by 75 publications

(56 citation statements)

References 31 publications

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“…Therefore, genetics can add a second dimension to the linear continuum of the macrophage polarization model. The expression of M1-associated genes such as STAT1 (FC = 1.62, FDR = 0.002), IRF1 (FC = 2.44, FDR = 2.83e-11), HIF-1A (FC = 1.44, FDR = 0.046), IL8 (FC = 2.2, FDR = 1.48e-4), CCL5 (FC = 5.83, FDR = 0.04), iNOS (FC = 2.26, FDR = 1.89e-4), CD38 (FC = 1.91, FDR = 0.023) and CD14 (FC = 1.60, FDR = 0.005) were found to be significantly more expressed in this high responder group [22][23][24]55,56 LGMN (FC = −2.86, FDR = 1.14e-9) are more expressed in low NO − responders 18,[57][58][59][60] . It should also be noted that the expression of GATA3 and IRF4, known M2-associated TFs 23 , were predicted to be inhibited in the high responder group (or activated in low responder group) based on DE genes at 18 hours (Supplementary Table 4).…”

Section: Discussionmentioning

confidence: 82%

Transcriptomic Profiles of Monocyte-Derived Macrophages in Response to Escherichia coli is Associated with the Host Genetics

Emam

Cánovas

Islas‐Trejo

et al. 2020

Sci Rep

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Reactive Nitrogen Species (RNS) are a group of bactericidal molecules produced by macrophages in response to pathogens in a process called oxidative burst. Nitric oxide (NO −) is a member of RNS produced from arginine by inducible Nitric Oxide Synthase (iNOS) enzyme. The activity of iNOS and production of NO − by macrophages following stimulation is one of the indicators of macrophage polarization towards M1/proinflammatory. Production of NO − by bovine monocyte-derived macrophage (MDM) and mouse peritoneal macrophages has been shown to be strongly associated with host genetic with the heritability of 0.776 in bovine MDM and 0.8 in mouse peritoneal macrophages. However, the mechanism of genetic regulation of macrophage response has remained less explored. In the current study, the transcriptome of bovine MDMs was compared between two extreme phenotypes that had been classified as high and low responder based on NO − production. The results showed that 179 and 392 genes were differentially expressed (DE) between high and low responder groups at 3 and 18 hours after exposure to Escherichia coli, respectively. A set of 11 Transcription Factors (TFs) (STAT1,

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Section: Discussionmentioning

confidence: 82%

Transcriptomic Profiles of Monocyte-Derived Macrophages in Response to Escherichia coli is Associated with the Host Genetics

Emam

Cánovas

Islas‐Trejo

et al. 2020

Sci Rep

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“…GPNMB is also involved in M2 polarization of macrophages. In GPNMB-knockdown mice, M2 polarization from bone marrow-derived macrophages was inhibited, while M1 polarization and the secretion of IL-1β and TNF-α were enhanced (35). Therefore, it is possible that lower level of GPNMB in the presence of allele A can indirectly contribute to the expressions of multiple inflammatory cytokines including IL-6, which is important in the pathogenesis of Takayasu arteritis.…”

Section: Discussionmentioning

confidence: 99%

Multi-disease associated risk locus inIL6represses the anti-inflammatory geneGPNMBthrough chromatin looping and recruiting MEF2-HDAC complex

Kong

Sawalha

2019

Preprint

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We have previously revealed a genetic association between Takayasu arteritis and a non-coding genetic variant in an enhancer region within IL6 (rs2069837 A/G). The risk allele in this variant (allele A) has a protective effect against chronic viral infection and cancer. Using a combination of experimental and bioinformatics tools, we identified the monocyte/macrophage anti-inflammatory gene GPNMB, ~520kb away, as a target gene regulated by rs2069837. We revealed preferential recruitment of myocyte enhancer factor 2-histone deacetylase (MEF2-HDAC) repressive complex to the Takayasu arteritis risk allele. Further, we demonstrated suppression of GPNMB expression in monocyte-derived macrophages from healthy individuals with the AA compared to AG genotype, which was reversed by histone deacetylase inhibition. Our data suggest that the A allele in rs2069837 represses the expression of GPNMB by recruiting MEF2-HDAC complex, enabled through a long-range intra-chromatin looping mediated by CTCF. Suppression of this anti-inflammatory gene might mediate increased susceptibility in Takayasu arteritis and enhance protective immune responses in chronic infection and cancer. Our data highlight long-range chromatin interactions in functional genomic studies.

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“…To effectively repair bone defects duo to tumour resection, bone biomaterials should provide appropriate media for angiogenesis and osteogenesis (29). In vitro, M2 macrophages directly regulate osteogenic differentiation of bone marrow mesenchymal stem cells (30,31). The mechanism may be due to proregenerative cytokines produced by M2 macrophages, such as TGF-β, VEGF, Ang-1 and IFG-1 (32).…”

Section: Introductionmentioning

confidence: 99%

Graphene-Modified CePO4 Nanorods Effectively Treat Breast Cancer-Induced Bone Metastases and Regulate Macrophage Polarization to Improve Osteo-Inductive Ability

Liu

et al. 2020

Preprint

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Background Breast cancer bone metastasis has become one of the most common complications; however, it may cause cancer recurrence and bone nonunion and local bone defects.Methods Herein, In vitro, we verified the effect of bioscaffold materials on cell proliferation and apoptosis through CCK8 experiment, staining of living dead cells, and flow cytometry. We used immunofluorescence technology and flow cytometry to verified whether bioscaffold materials regulated the polarization of macrophages, and we use ALP staining, alizarin red staining and PCR to verify whether the bioscaffold material promotes bone regeneration. In vivo, we once again studied the effect of bioscaffold materials on tumors by measuring tumor volume in mice, Tunel staining, and caspase-3 immunofluorescence. We also constructed a mouse skull ultimate defect model to verify the effect on bone regeneration.Results Graphene oxide (GO) nanoparticles, hydrated CePO4 nanorods and bioactive chitosan (CS) are combined to form a bioactive multifunctional CePO4/CS/GO scaffolds, which has the following characteristics such as photothermal therapy to kill tumors, macrophage polarization promotes blood vessel formation and induces bone formation. The CePO4/CS/GO scaffold activates the caspase-3 proteasein local tumor cells, thereby lysing DNA between nucleosomes and causing apoptosis. On the one hand, the as-released Ce3+ ions promote the M2 polarization of macrophages, which secretes vascular endothelial growth factor (VEGF) and Arginase-1 (Arg-1), which promotes angiogenesis. On the other hand, the as-released Ce3+ ions also activated BMP-2/Smad signaling pathway that facilitated bone tissue regeneration.Conclusion the multifunctional CePO4/CS/GO scaffolds may become a promising platform for therapy of breast cancer bone metastases.

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Glycoprotein non-metastatic melanoma protein b (Gpnmb) is highly expressed in macrophages of acute injured kidney and promotes M2 macrophages polarization

Cited by 75 publications

References 31 publications

Transcriptomic Profiles of Monocyte-Derived Macrophages in Response to Escherichia coli is Associated with the Host Genetics

Transcriptomic Profiles of Monocyte-Derived Macrophages in Response to Escherichia coli is Associated with the Host Genetics

Multi-disease associated risk locus inIL6represses the anti-inflammatory geneGPNMBthrough chromatin looping and recruiting MEF2-HDAC complex

Graphene-Modified CePO4 Nanorods Effectively Treat Breast Cancer-Induced Bone Metastases and Regulate Macrophage Polarization to Improve Osteo-Inductive Ability

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