Glycoprotein IIb-IIIa-liposomes bind fibrinogen but do not undergo fibrinogen-mediated aggregation

Abstract

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Liposomes and Blood Cells: A Flow Cytometric Study

“…Fusion could explain the lack of enhanced platelet aggregation with or without ADP shown by RGD liposomes (Rybak and Renzulli, 1993) and GPIIbIIIa-loaded liposomes (Sloan et al, 2000). Similarly, it could offer an alternative explanation for the effectiveness and localization of synthetic phospholipids that promote procoagulant activity on damaged vessels (Galan et al, 1998), the clot accumulation of plasminogen-bearing liposomes (Heeremans et al, 1998) and the improved thrombolysis of streptokinasecontaining liposomes (Perkins et al, 1997).…”

Echicetin, a GPIb-binding snake C-type lectin from Echis carinatus, also contains a binding site for IgMκ responsible for platelet agglutination in plasma and inducing signal transduction

“…It was shown before that GPIIb/IIIa can be activated and can bind fibrinogen without aggregation necessarily occurring. 16,25 FITC-fibrinogen binding to platelets activated by echicetin-IgM was examined by flow cytometric analysis and was significantly increased on activated platelets (Figure 4).…”

Development of a Point of Care Lateral Flow Device for Measuring Human Plasma Fibrinogen

“…Fibrinogen (Factor I) is a 340 kDa plasma glycoprotein. , When cleaved by thrombin as a result of clotting activation, it forms fibrin monomers that aggregate into an insoluble, cross-linked polymer structure. Fibrinogen is involved in primary hemostasis, platelet aggregation, and leukocyte−endothelial cell interactions . Fibrinogen concentration (and associated changes in whole blood viscosity) has been reported to be associated with the risk of cardiovascular heart disease, − recurrent stroke, myocardial infarction, venous thrombosis, , disseminated intravascular coagulation (DIC), systemic fibrinolysis, pancreatitis, and severe hepatic dysfunction.…”