Glycoprotein IIB/IIIA‐Inhibition and Microcirculatory Alterations During Experimental Endotoxemia—An Intravital Microscopic Study in the Rat

Walther, Andreas; Czabanka, Marcus; Gebhard, Martha Maria; Martin, Eike

doi:10.1080/10739680490266216

Cited by 22 publications

(26 citation statements)

References 50 publications

(77 reference statements)

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“…Otherwise, no additional risk of infection has been associated with abciximab (Reopro package insert). In fact, there is in vitro and murine model evidence that glycoprotein IIb/IIIa inhibition protects against endothelial cell dysfunction and leukocyte adherence to the vascular wall during experimental endotoxemia (88,190).…”

Section: Monoclonal Antibodies For Cardiovascular Diseasementioning

confidence: 99%

Infectious Complications Associated with Monoclonal Antibodies and Related Small Molecules

Salvaña

Salata

2009

Clin Microbiol Rev

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SUMMARY Biologics are increasingly becoming part of routine disease management. As more agents are developed, the challenge of keeping track of indications and side effects is growing. While biologics represent a milestone in targeted and specific therapy, they are not without drawbacks, and the judicious use of these “magic bullets” is essential if their full potential is to be realized. Infectious complications in particular are not an uncommon side effect of therapy, whether as a direct consequence of the agent or because of the underlying disease process. With this in mind, we have reviewed and summarized the risks of infection and the infectious disease-related complications for all FDA-approved monoclonal antibodies and some related small molecules, and we discuss the probable mechanisms involved in immunosuppression as well as recommendations for prophylaxis and treatment of specific disease entities.

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Section: Monoclonal Antibodies For Cardiovascular Diseasementioning

confidence: 99%

Infectious Complications Associated with Monoclonal Antibodies and Related Small Molecules

Salvaña

Salata

2009

Clin Microbiol Rev

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“…In addition, intravital microscopy has also been used to test novel prophylactic and therapeutic approaches that aim to prevent or attenuate manifestations of sepsis-associated microvascular disorders and cellular dysfunctions. In the mesentery, microcirculatory observations have focused on capillary obstruction, capillary or arteriolar density, microvessel reactivity and leukocyte-endothelial interactions in post-capillary venules (Harris, 2006;Kim & Harris, 2006;Nakagawa et al, 2006Nakagawa et al, , 2007Schimidt et al, 1997;Smalley et al, 2000;Walther et al, 2004;Woodman et al, 2000). A representative photomicrograph of rat mesenteric microcirculation is shown in Figure 3.…”

Section: Intravital Microscopy In the Assessment Of Microcirculationmentioning

confidence: 99%

“…Microcirculatory dysfunctions, as seen in humans (De Backer & Dubois, 2001;De Backer et al, 2002;Groner et al, 1999;Trzeciak et al, 2007), have been shown to occur in most experimental models of sepsis (Baveja et al, 2002;Kamoun et al, 2005;Kim & Harris, 2006;Kubo et al, 1999;Nakagawa et al, 2006Nakagawa et al, , 2007Nakajima et al, 2001;Schimidt et al, 1997;Smalley et al, 2000;Waisman et al, 2006;Walther et al, 2004;Woodman et al, 2000). Endotoxin infusion is a widely used experimental model (Kim & Harris, 2006;Schimidt et al, 1997;Smalley et al, 2000;Nakajima et al, 2001).…”

Section: Intravital Microscopy In the Assessment Of Microcirculationmentioning

confidence: 99%

“…Endotoxin infusion is a widely used experimental model (Kim & Harris, 2006;Schimidt et al, 1997;Smalley et al, 2000;Nakajima et al, 2001). Increased leukocyte-endothelial interactions and protein leakage in mesenteric microvessels have been shown to occur after acute endotoxemia in rats (Schimidt et al, 1997;Woodman et al, 2000) and cats (Walther et al, 2004). However, there are two major concerns regarding this experimental model: 1) clinical sepsis typically evolves over many days, in contrast to studies on the early effects (1 to 6 hours) of endotoxin administration, and 2) rats are generally more resistant to the effects of endotoxin.…”

Section: Intravital Microscopy In the Assessment Of Microcirculationmentioning

confidence: 99%

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An Animal Model of Sepsis in Appendicitis: Assessment of the Microcirculation

Tatebe¹,

Aikawa²,

Jukemura³

et al. 2012

Appendicitis - A Collection of Essays From Around the World

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“…We know from the literature that endothelial damage during early endotoxemia is leukocyte-independent and can be inhibited by glycoprotein IIb/IIIa receptor antagonism and by nitric oxide [4,5].…”

Section: Introductionmentioning

confidence: 99%

Spermine and Endothelial Damage During Endotoxemia

et al. 2008

Self Cite

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Diazenolate-NO-donors like spermine-NONOate use spermine as a nitric oxide releasing carrier. As one of the naturally occurring polyamines, spermine belongs to the most potent platelet aggregation inhibitors among the polyamines. Recent publications describe a dominating role for platelets and nitric oxide in the setting of endothelial dysfunction during endotoxemia. It is unknown whether spermine as single agent, beside his function as nitric oxide releasing carrier, exerts effects on macromolecular leakage during early endotoxemia. In male Wistar rats, macromolecular efflux was determined in mesenteric postcapillary venules using intravital microscopy at baseline, 60 and 120 min after the start of the experiment. In the first part of the experiments we investigated the effects of spermine and spermine-NONOate during leukocyte-independent states of endotoxemia, using the L- and P-selectin inhibitor fucoidin to suppress leukocyte-endothelial-interaction. In the second part we investigated the effects of spermine on endothelial damage during endotoxemia without co-administration of fucoidin. Combined treatment of animals with spermine and fucoidin was as effective as treatment with spermine-NONOate and fucoidin alone. Spermine and fucoidin as single agents had no effects on macromolecular efflux during endotoxin challenge. Combined treatment of animals with spermine and fucoidin reduce macromolecular efflux to the same degree like treatment with spermine-NONOate and fucoidin. A currently unknown interaction between fucoidin and spermine leads to a significant reduction in macromolecular efflux.

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Glycoprotein IIB/IIIA‐Inhibition and Microcirculatory Alterations During Experimental Endotoxemia—An Intravital Microscopic Study in the Rat

Abstract: GP IIb/IIIa inhibition, using abciximab, protects against endothelial dysfunction and an increase in leukocyte adherence to the vascular wall during experimental endotoxemia. The protective properties of abciximab on microcirculation seemed to be leukocyte-independent.

Cited by 22 publications

References 50 publications

Infectious Complications Associated with Monoclonal Antibodies and Related Small Molecules

Infectious Complications Associated with Monoclonal Antibodies and Related Small Molecules

An Animal Model of Sepsis in Appendicitis: Assessment of the Microcirculation

Spermine and Endothelial Damage During Endotoxemia

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