Glycoprotein IIb-IIIa complex in platelets of patients and heterozygotes of Glanzmann's thrombasthenia

Herrmann, F.; Meyer, M.; Gogstad, Geir O.; Solum, Nils Olav

doi:10.1016/0049-3848(83)90063-4

Cited by 14 publications

(6 citation statements)

References 21 publications

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“…GP IIb-IIIa complex was measured by electroimmunoassay using monospecific anti-GP IIb-IIIa antiserum (30) Platelet fibrinogen and von Willebrand factor were also determined by electroimmunoassay using monospecific antibodies. Crossed immunoelectrophoresis (CIE) of Thiton X-L00-solublized platelets were performed according to Hagen…”

Section: Immunochemical M Etho Dsmentioning

confidence: 99%

Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

Meyer¹,

Kirchmaier

Schirmer

et al. 1991

Thromb Haemost

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SummaryA patient with idiopathic thrombocytopenic purpura developed after splenectomy a thrombasthenia-like severe haemor-rhagic diathesis characterized by a normal or subnormal platelet count, prolonged bleeding time, strongly reduced platelet adhesion to glass and defective platelet aggregation in response to ADP and collagen. In contrast to hereditary thrombasthenia membrane glycoproteins (GP) lib and Ilia were normally present in the patient’s platelets. Immunoelectrophoretic analysis revealed an abnormal behaviour of the patient’s GP IIb-IIIa complex. Autoantibodies against GP IIb-IIIa were detected in Triton-extracted washed platelets. Incubation of normal platelets with plasma from the patient resulted in a similar immunoelectrophoretic abnormality of the GP IIb-IIIa complex indicating that bound autoantibodies (IgG) are responsible for the abnormal immunoelectrophoretic behaviour of the patient’s GP IIb-IIIa complex. Platelet fibrinogen was severely reduced similar to classical thrombasthenia suggesting that the GP IIb-IIIa complex is involved in platelet fibrinogen storage.

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Section: Immunochemical M Etho Dsmentioning

confidence: 99%

Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

Meyer¹,

Kirchmaier

Schirmer

et al. 1991

Thromb Haemost

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“…15 Patients with type I disease are usually severely affected; those with the type II or heterozygotes form 16 may have mild or no symptoms. Although Caen 17 stressed the amelioration of bleeding manifestations with age, Reichert et al 5 have confirmed that bleeding manifestations persist into adulthood.…”

Section: Discussionmentioning

confidence: 99%

Glanzmann's Thrombasthenia Type II in a Saudi Child: A Case Report

1990

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“…9 thrombasthenic patients from 7 families have been examined. Most of them were previously described (13,14).…”

Section: Patientsmentioning

confidence: 99%

“…Quantitative estimation of GP II b-Illa complex was accomplished by rocket immunoelectrophoresis with monospecific anti-GP lib-Ilia complex-antiserum as previously described (14).…”

Section: Unreduced-reduced Two-dimensional Sds-polyacrylamide Gel Elementioning

confidence: 99%

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Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

Meyer

Herrmann

1985

Thromb Haemost

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SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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Glycoprotein IIb-IIIa complex in platelets of patients and heterozygotes of Glanzmann's thrombasthenia

Cited by 14 publications

References 21 publications

Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

Glanzmann's Thrombasthenia Type II in a Saudi Child: A Case Report

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

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