Glycoprotein GP57/51 of Trypanosoma cruzi: structural and conformational  epitopes defined with monoclonal antibodies

Murta, A. C. M.; Leme, V. C. M.; Milani, S.; Travassos, Luiz R.; Scharfstein, Júlio

doi:10.1590/s0074-02761988000500038

Cited by 6 publications

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“…But while there was an apparent difference in the recognition by T cells of separated antigens, none of the specific antigen bands were recognized preferentially by sera of patients with one clinical form vs. another. These studies further describe a glycoprotein component of EPI which migrates diffusely between 57 and 51 kD (GP57/51) (Scharfstein et al, 1986;Murta et al, 1988). GP57/51 antigen is recognized by all patients' sera and cells, and a given patient's PBMC responses to this purified antigen correlate with their responsiveness to crude EPI.…”

Section: Biology Of Trypanosoma Cruzimentioning

confidence: 97%

The fundamental nature of Chagas' disease:A view provided by immune responses and idiotypes.

Colley

Howard

1991

Jap. J. Trop. Med. Hyg.

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Chagas' disease is caused by the protozoan Trypanosoma cruzi which infects 10-15 million people in endemic areas throughout Latin America and is naturally transmitted by insect vectors of the family Reduviidae. Infection can also occur by congenital passage, oral ingestion, laboratory accident, and in organ transplants and blood transfusions. There are 3 life-cycle forms of T. cruzi. Epimastigotes multiply in the midgut of the insect vector, differentiate in the hind-gut into infectious trypomastigotes, patients who die of unrelated causes have identical (but less intense) lesions. Responses against T. cruzi antigens, their immunoregulation, and responses against related idiotypes (Ids) correlate with the presence of the different clinical forms of the infection. Although there are numerous findings of autoimmune lymphocyte and antibody reactivities in chagasic patients, a causal relationship is always difficult to prove. Chagasic patients' peripheral blood mononuclear cells (PBMC) respond to T. cruzi epimastigote antigens (EPI) with varying vigor. Almost all low responders are Cpatients, and their responses are usually augmented by removal of adherent macrophage suppressor cells or the addition of indomethacin. Chronic I-patients are medium or high responders, and exhibit little adherent cell-mediated immunoregulation. Western blotting

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Section: Biology Of Trypanosoma Cruzimentioning

confidence: 97%

The fundamental nature of Chagas' disease:A view provided by immune responses and idiotypes.

Colley

Howard

1991

Jap. J. Trop. Med. Hyg.

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“…Subcellular localization [9] and proteomics [10] experiments have shown reservosomes to contain large amounts of a cysteine proteinase, known as cruzipain [11] or GP57/51 [12]. The native GP57/51 has been isolated from epimastigotes and used to generate a monoclonal antibody (mAb) [13]. Subcellular localization experiments demonstrated the presence of this protein in vesicles of the endosomal/lysosomal system and close to the flagellar pocket [12, 14].…”

Section: Introductionmentioning

confidence: 99%

mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes ofTrypanosoma cruziEpimastigotes

Batista

Medeiros

Eger

et al. 2014

BioMed Research International

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Reservosomes are large round vesicles located at the posterior end of epimastigote forms of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. They are the specific end organelles of the endocytosis pathway of T. cruzi, and they play key roles in nutrient uptake and cell differentiation. These lysosome-like organelles accumulate ingested macromolecules and contain large amounts of a major cysteine proteinase (cruzipain or GP57/51 protein). Aim of this study was to produce a monoclonal antibody (mAb) against a recombinant T. cruzi cruzipain (TcCruzipain) that specifically labels the reservosomes. BALB/c mice were immunized with purified recombinant TcCruzipain to obtain the mAb. After fusion of isolated splenocytes with myeloma cells and screening, a mAb was obtained by limiting dilution and characterized by capture ELISA. We report here the production of a kappa-positive monoclonal IgG antibody (mAb CZP-315.D9) that recognizes recombinant TcCruzipain. This mAb binds preferentially to a protein with a molecular weight of about 50 kDa on western blots and specifically labels reservosomes by immunofluorescence and transmission electron microscopy. The monoclonal CZP-315.D9 constitutes a potentially powerful marker for use in studies on the function of reservosomes of T. cruzi.

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Induction of B- and T-cell responses to cruzipain in the murine model of Trypanosoma cruzi infection

Schnapp

Eickhoff

Scharfstein

et al. 2002

Microbes and Infection

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Glycoprotein GP57/51 of Trypanosoma cruzi: structural and conformational epitopes defined with monoclonal antibodies

Cited by 6 publications

References 0 publications

The fundamental nature of Chagas' disease:A view provided by immune responses and idiotypes.

The fundamental nature of Chagas' disease:A view provided by immune responses and idiotypes.

mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes ofTrypanosoma cruziEpimastigotes

Induction of B- and T-cell responses to cruzipain in the murine model of Trypanosoma cruzi infection

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