Glycoprotein gene sequence variation in rhesus monkey rhadinovirus

Abstract

Gene sequences for seven glycoproteins from 20 independent isolates of rhesus monkey rhadinovirus (RRV) and of the corresponding seven glycoprotein genes from nine strains of the Kaposi’s sarcoma-associated herpesvirus (KSHV) were obtained and analyzed. Phylogenetic analysis revealed two discrete groupings of RRV gH sequences, two discrete groupings of RRV gL sequences and two discrete groupings of RRV gB sequences. We called these phylogenetic groupings gHa, gHb, gLa, gLb, gBa and gBb. gHa was always paired with gLa and gHb was always paired with gLb for any individual RRV isolate. Since gH and gL are known to be interacting partners, these results suggest the need of matching sequence types for function of these cooperating proteins. gB phylogenetic grouping was not associated with gH/gL phylogenetic grouping. Our results demonstrate two distinct, distantly-related phylogenetic groupings of gH and gL of RRV despite a remarkable degree of sequence conservation within each individual phylogenetic group.

Construction and Manipulation of a New Kaposi's Sarcoma-Associated Herpesvirus Bacterial Artificial Chromosome Clone

“…Importantly, sequence reads were represented equally across the LUR portion of BAC16, indicating a lack of a large-scale duplication. In previous work, several loci of the KSHV genome were sequenced via PCR amplification directly from JSC-1 cells, including the ORF26 region, ORF75-E, gB, gH, gL, gM, gN, K8.1, and ORF68 (53,58). Comparison of these locus sequences with the BAC16 sequence showed 100% identity, with the exception of a synonymous point mutation at A242 of ORF75 (data not shown).…”

Rhesus Rhadinovirus Infection of Rhesus Fibroblasts Occurs through Clathrin-Mediated Endocytosis

“…While we have provided evidence that clathrin-mediated endocytosis is the major pathway mediating RRV entry into RFs, it is entirely possible that other pathways might also be involved, particularly in other cell types. This is even more likely considering the recent discovery of sequence variation in RRV glycoproteins (33).…”

Complete Genome Sequence of Pig-Tailed Macaque Rhadinovirus 2 and Its Evolutionary Relationship with Rhesus Macaque Rhadinovirus and Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus

“…It was previously noted that phylogenetic clustering of the interacting glycoprotein pairs ORF22 (gH) and ORF47 (gL) was distinct between RRV isolates similar to RRV26-95 and isolates similar to RRV17577. Furthermore, the phylogenetic clustering of the independent glycoprotein ORF8 gB sequences was not associated with the gH/gL paired clustering (60). An alignment and protein maximum likelihood analysis of the ORF22 gH and ORF47 gL homologs of MneRV2 showed a similar phylogenetic relationship to the homologs of RRV26-95 and MfuRV2, as seen with ORFK1, ORFK8.1, and ORF8 gB above (Fig.…”

“…11B). The gH and gL genes from the RRV isolates clustering with RRV17577 lacked sequence variation, showing no evidence of unusual evolutionary pressure (60). This suggests that unlike MneRV2 and RRV26-95, the RRV17577 gH and gL variants were derived from a gene capture event of one or both of the interacting genes from a more distantly related herpesvirus.…”