Glycoprotein E2 of classical swine fever virus expressed by baculovirus induces the protective immune responses in rabbits

Zhang, Huawei; Li, Xiangmin; Peng, Guiqing; Tang, Chenkai; Zhu, Shixuan; Qian, Suhong; Xu, Jinfang; Qian, Ping

doi:10.1016/j.vaccine.2014.10.003

Cited by 27 publications

(22 citation statements)

References 29 publications

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“…E rns and E2 are envelope glycoproteins located on the surface of the virus. Both of them could elicit protective immunity against CSFV challenge in pigs and E2 is a better candidate to be incorporated in genetically engineered CSFV vaccines [7][8][9]28,29]. Recently, Gavrilov et al have reported that glycosylation of E rns and E2 is essential for their immunogenicity and unglycosylated proteins expressed by baculovirus failed to induce protection against CSFV infection [30].…”

Section: Discussionmentioning

confidence: 99%

The swine CD81 enhances E2-based DNA vaccination against classical swine fever

Zhou

et al. 2015

Vaccine

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Section: Discussionmentioning

confidence: 99%

The swine CD81 enhances E2-based DNA vaccination against classical swine fever

Zhou

et al. 2015

Vaccine

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“…The L. plantarum/pYG-E2-T␣1 and L. plantarum/pYG-E2 cells that were induced by xylose overnight were centrifuged and resuspended in suspension buffer (0.2 mol/liter NaHCO 3 , 5% casein acid hydrolysate, 0.5% sucrose) to a concentration of ca. 10 10 CFU/ml. Then, the recombinant strain suspensions were fully mixed with pig feed raw materials, and a manual extruder was used to cold extrude the mixtures into particles (0.5 cm by 1 cm on average) containing, on average, ca.…”

Section: Methodsmentioning

confidence: 97%

“…The genome of CSFV consists of a positivestranded RNA molecule of about 12.3 kb, encoding a single open reading frame that is translated into a 3,898-amino-acid polyprotein, giving rise to different CSFV proteins after coprocessing and after translational processing (1,2). Of these, the E2 structural protein encompasses major antigenic domains and cytotoxic T lymphocyte (CTL) epitopes, suggesting a promising candidate for an immunogen with the capacity to induce neutralizing antibodies and CTL activities against CSFV (3)(4)(5)(6)(7)(8)(9)(10). CSFV often causes severe and lethal disease in pigs, resulting in enormous economic losses in pig industries (11,12).…”

mentioning

confidence: 99%

Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant

Guan

Liu

et al. 2015

Appl Environ Microbiol

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e Classical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFVneutralizing antibodies and cytotoxic T lymphocyte (CTL) activities in vivo. Thymosin ␣-1 (T␣1), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineered Lactobacillus plantarum bacteria expressing CSFV E2 protein alone (L. plantarum/pYG-E2) and in combination with T␣1 (L. plantarum/pYG-E2-T␣1) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinant L. plantarum/pYG-E2 and L. plantarum/pYG-E2-T␣1 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (P < 0.05) in the levels of immune responses were observed between L. plantarum/pYG-E2-T␣1 and L. plantarum/pYG-E2, suggesting a better immunogenicity of L. plantarum/pYG-E2-T␣1 as a result of the T␣1 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinant Lactobacillus microecological agent expressing CSFV E2 protein combined with T␣1 as an adjuvant provides a promising strategy for vaccine development against CSFV. C lassical swine fever virus (CSFV), a member of the Pestivirus genus of the Flaviviridae family, is a small, enveloped, singlestranded RNA virus. The genome of CSFV consists of a positivestranded RNA molecule of about 12.3 kb, encoding a single open reading frame that is translated into a 3,898-amino-acid polyprotein, giving rise to different CSFV proteins after coprocessing and after translational processing (1, 2). Of these, the E2 structural protein encompasses major antigenic domains and cytotoxic T lymphocyte (CTL) epitopes, suggesting a promising candidate for an immunogen with the capacity to induce neutralizing antibodies and CTL activities against CSFV (3-10). CSFV often causes severe and lethal disease in pigs, resulting in enormous economic losses in pig industries (11,12). Therefore, the development of effective vaccines against CSFV infection is an issue of growing importance.For the development of CSFV vaccines, the roles of neutralizing antibody-based humoral immune responses (13, 14) and CTL-based cellular immune responses (15-20) have been both highlighted. Furthermore, the invasion of CSFV often initiates at mucosal surfaces, particularly intestinal tissues, and thus, vaccination inducing IgA-based protective mucosal immunity via the mucosal approach could effectively prevent the virus from entering the body via mucosa and its further spread to the systemic circulation. Taken together, the design of new vaccines ...

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“…E2 subunit vaccines have been confirmed to induce sufficient CSFV specific antibodies and provide complete protection against homologous CSFV in rabbits [18] and pigs [19,20]. Commercial CSFV E2 subunit vaccine Porcilis® Pesti derived from genotype 1 fail to elicit complete protection against heterologous strains of genotype 2.1 [18], while by booster immunization another commercial vaccine TWJ-E2® (genotype 1) was reported to provide complete protection against heterologous strains of genotype 2 [21]. However, these subunit vaccines usually require large multiple doses to induce the comparable CSFV-specific protective immunity as C-strain LAVs [22].…”

Section: Introductionmentioning

confidence: 99%

Identification of E2 with improved secretion and immunogenicity against CSFV in piglets

Wang

Han

et al. 2020

BMC Microbiol

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Background: Outbreaks of Classical swine fever virus (CSFV) cause significant economic losses in the swine industry. Vaccination is the major method to prevent and control the disease. As live attenuated vaccines fail to elicit differentiable immunity between infected and vaccinated animals, subunit vaccine was considered as an alternative candidate to prevent and eradicate CSFV. Subunit vaccines present advantages in DIVA immunogenicity and safety. The technology was limited due to the low yield and the high cost with multiple and large doses. The native E2 signal peptide has not been well defined before. Here, the aim of this study is to develop a cost-effective and efficacious E2 vaccine candidate against CSFV with signal peptide and E2 sequence selection. Results: A novel CSFV E2 sequence (E2ZJ) was identified from an epidemic strain of Zhejiang for outstanding secretion in baculovirus and enhanced immunogenicity. E2 secretion induced with the selected signal peptide, SPZJ (SP23), increase at least 50% as compared to any other signal peptides tested. Besides, unique antigenic features were identified in E2ZJ. As indicated with immunized sera in IFA against CSFV infection, E2ZJ elicited CSFV antibodies at the earlier stage than other E2 types tested in mice. Moreover, higher level of neutralizing and CSFV antibodies against CSFV with E2ZJ was detected than other E2s with the same dosage at 28 dpi. Further, E2ZJ successfully elicited neutralizing immunity in piglets. A single dose of 5 μg of E2ZJ was sufficient to induce protective antibodies against CSFV in piglets and provided 100% protection against lethal virus challenge. Conclusions: Our studies provide evidence that E2ZJ guided by a novel E2 signal peptide (SPZJ) was efficiently secreted and presented significantly improved immunogenicity than conventional E2 vaccines. Moreover, a single dose of 5 μg E2ZJ is efficacious against CSFV in piglets.

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Glycoprotein E2 of classical swine fever virus expressed by baculovirus induces the protective immune responses in rabbits

Cited by 27 publications

References 29 publications

The swine CD81 enhances E2-based DNA vaccination against classical swine fever

The swine CD81 enhances E2-based DNA vaccination against classical swine fever

Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant

Identification of E2 with improved secretion and immunogenicity against CSFV in piglets

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