Glycophorin as a Possible Receptor for Plasmodium Falciparum

Pasvol, Geoffrey; Jungery, Michele; Weatherall, D. J.; Parsons, Stephen; Anstee, David J.; Tanner, Michael

doi:10.1016/s0140-6736(82)90157-x

Cited by 124 publications

(68 citation statements)

References 13 publications

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“…It is interesting that the glycophorins, the other major group of glycoproteins of the erythrocyte membrane, are probably involved in invasion by P. falciparum (28)(29)(30)(31). The glycophorins are not involved in invasion by P. knowlesi; trypsin treatment of erythrocytes that cleaves glycophorin A and En(a-) erythrocytes that lack glycophorin A are invaded normally by P. knowlesi (28).…”

Section: Resultsmentioning

confidence: 99%

A monoclonal antibody to rhesus erythrocyte band 3 inhibits invasion by malaria (Plasmodium knowlesi) merozoites.

Miller¹,

Hudson²,

Rener³

et al. 1983

J. Clin. Invest.

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A B S T R A C T Receptors on erythrocytes and malaria parasites mediate specific attachment and junction formation between these cells that lead to invasion of the erythrocytes. We identified a monoclonal antibody A9 and its subelone A9D3 that bound to rhesus erythrocytes and blocked invasion of the erythrocytes by Plasmodium knowlesi merozoites. The monoclonal antibodies did not block attachment, the initial step in invasion, although swelling and crenation of the erythrocyte, which normally occur after attachment, were rarely observed in the presence of antibody. The monoclonal antibody immunoprecipitated rhesus erythrocyte band 3. It bound to erythrocytes of another Old World monkey, the kra monkey, but not to erythrocytes of New World monkeys, chimpanzees, or man. Since the antibody did not bind to human erythrocytes, we could test for nonspecific toxicity to the parasite by studying the effect of the ascites and purified antibody on invasion of human erythrocytes. The antibody caused a minimal reduction in invasion of human erythrocytes, a reduction no greater than that seen with an unrelated monoclonal antibody. Further evidence that the inhibition was specific came from study of Fab fragments of A9D3. Column-purified Fab fragments reduced invasion of rhesus erythrocytes without affecting invasion of human erythrocytes. Fab fragments preabsorbed with rhesus erythrocytes did not inhibit invasion. From the above data, we conclude that band 3 is involved in a stage in the invasion process after initial recognition.

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Section: Resultsmentioning

confidence: 99%

A monoclonal antibody to rhesus erythrocyte band 3 inhibits invasion by malaria (Plasmodium knowlesi) merozoites.

Miller¹,

Hudson²,

Rener³

et al. 1983

J. Clin. Invest.

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“…Recently several studies have focused on erythrocyte sialic acid and its role in invasion of erythrocytes by the human malaria parasite Plasmodium falciparum (1)(2)(3)(4)(5)(6)(7). Some studies have shown that different lines of P. falciparum vary in their dependence on erythrocyte sialic acid for effective invasion of red blood cells (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Evidence for a switching mechanism in the invasion of erythrocytes by Plasmodium falciparum.

Dolan

Miller²,

Wellems³

1990

J. Clin. Invest.

197

165

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“…Different species of Plasmodium use different ligands. Erythrocyte glycophorin A has been proposed as a ligand for invasion by Plasmodiumfalciparum (2)(3)(4)(5)(6)(7). Evidence for this derives in part from the fact that En(a-) erythrocytes, which lack glycophorin A, are not invaded as well as normal erythrocytes (2,7).…”

Section: Introductionmentioning

confidence: 99%

Falciparum malaria parasites invade erythrocytes that lack glycophorin A and B (MkMk). Strain differences indicate receptor heterogeneity and two pathways for invasion.

Hadley¹,

Klotz²,

Pasvol³

et al. 1987

J. Clin. Invest.

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149

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Glycophorin as a Possible Receptor for Plasmodium Falciparum

Cited by 124 publications

References 13 publications

A monoclonal antibody to rhesus erythrocyte band 3 inhibits invasion by malaria (Plasmodium knowlesi) merozoites.

A monoclonal antibody to rhesus erythrocyte band 3 inhibits invasion by malaria (Plasmodium knowlesi) merozoites.

Evidence for a switching mechanism in the invasion of erythrocytes by Plasmodium falciparum.

Falciparum malaria parasites invade erythrocytes that lack glycophorin A and B (MkMk). Strain differences indicate receptor heterogeneity and two pathways for invasion.

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