Glycopattern analysis and structure of the egg extra-cellular matrix in the Apennine yellow-bellied toad, &lt;i&gt;Bombina pachypus (Anura: Bombinatoridae)&lt;/i&gt;

Scillitani, Giovanni; Moramarco, Antonietta; Rossi, Roberta; Mastrodonato, María

doi:10.5603/fhc.2011.0043

Cited by 13 publications

(17 citation statements)

References 46 publications

(92 reference statements)

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“…Comparison of results among different studies is not always easy, because of different methods and terminology. When compared to Bombina pachypus , which we studied previously (Scillitani et al, ), B. bufo and B. balearicus have a lower number of jelly layers, but a comparable number of glycan types as evidenced by lectin histochemistry. Similarly, a survey of the available literature about ECM composition in bufonid toads (Arranz et al, ; Coppin et al, ; Farias et al, ; Kawai and Anno, ; Morelle et al, ; Omata, ; Reddy and Seshadri, ) indicates that in the two species we studied there are the main residuals found in other species, such as galactose, N ‐acetylgalactosamine, N ‐acetylglucosamine, sialic acid, fucose, and mannose.…”

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 77%

“…This is confirmed by lectin analysis, that in both species indicates binding to RCA‐1 and ConA lectins, similar to what was observed in the FE layer. It has been suggested that N ‐linked glycans, in particular lactosamine chains, have a structural function, orientate regulatory molecules, and are involved in water retention (Scillitani et al, ). Anyway, lactosaminylated residuals in O ‐linked chains cannot be excluded, as their presence in egg jelly was reported (Coppin et al, ; Morelle et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…It is unclear why the number of layers changes from one species to another, and how this affects egg's development (Altig and McDiarmid, ). In a previous contribution (Scillitani et al, ), we studied the composition of the ECM of the Italian yellow‐bellied toad, Bombina pachypus and found five different layers in the jelly coat, differing among them in histochemical staining, lectin binding, and ultrastructure. This is a relatively high number of jelly coats for Anura (Altig and McDiarmid, ; Carroll et al, ; Salthe, ), and we hypothesized that it could be related to protection against pathogens or chemicals, as it is believed that species with thicker jelly‐coated eggs are less exposed to infection and chemical penetration than those with thinner coats (Gomez‐Mestre and Keller, ; Marquis et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Histochemical and structural characterization of egg extra-cellular matrix in bufonid toads,Bufo bufoandBufotes balearicus: Molecular diversity versus morphological uniformity

Mentino

Mastrodonato

Rossi

et al. 2014

Microsc. Res. Tech.

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The extra-cellular matrix of fertilized eggs in the bufonid toads Bufo bufo and Bufotes balearicus was studied to clear the relationships between structural and molecular diversity. Histochemical (PAS, AB pH 2.5 and pH 1.0, Beta-elimination PAS) and lectin-histochemical (Con A, WGA, Succinyl-WGA, PNA, RCA-1, DBA, SBA, AAA, UEA-I, LTA) techniques were used and the observations were made under light and electron microscopy. Both species present a fertilization envelope (FE) and two jelly layers (J1 and J2). The fibers of J2 are shared among the eggs of a clutch in a jelly ribbon. The FE of both species presents neutral glycoproteins, mostly N-linked. In B. bufo there are also residuals of mannose and/or glucose and N-acetylglucosamine. In the FE fibers run parallel to egg's surface or are in bundles or looser hanks with no clear orientation. The J1 layer of both species presents sialosulfoglycoproteins, mostly O-linked, with lactosaminylated, galactosaminylated, glycosaminylated, and fucosylated residuals. A lower amount of galactosaminylated residuals is observed in B. balearicus in respect to B. bufo, whereas the opposite is seen in the amount of fucosylated residuals. The J2 layer is similar in composition to J1 but in B. balearicus there are no glucosaminylated residuals. J layers present fibers and granules that reduce towards J2 . Several microorganisms, in particular blue algae, are observed in the J2 layer of both species. In respect to other species, B. bufo and B. balearicus have a lower number of jelly layers, but a comparable number of glycan types.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histochemical and structural characterization of egg extra-cellular matrix in bufonid toads,Bufo bufoandBufotes balearicus: Molecular diversity versus morphological uniformity

Mentino

Mastrodonato

Rossi

et al. 2014

Microsc. Res. Tech.

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“…Details of the lectins employed, their concentrations and sugar specificities are given in Table . Lectin protocols followed manufacturer's instructions and were reported elsewhere (Scillitani et al ., ). After 1 h incubation at room temperature in the appropriate lectin solution in 4‐(2‐hydroxyethyl)‐1‐piperazine‐ethane‐sulphonic acid (HEPES), sections were rinsed in the same buffer and mounted in Fluoromount (Sigma‐Aldrich) for observation.…”

Section: Methodsmentioning

confidence: 97%

Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)

Carlucci

Mentino

Semeraro

et al. 2019

Journal of Fish Biology

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The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition. Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus. GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid. Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster. Functional and ecological implications of these findings are discussed.

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Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

Guglielmi,

Mastrodonato,

Semeraro

et al. 2024

Microscopy Res & Technique

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Aluminum (Al) is used in everyday life and present in food drugs, packaging, industry, and agriculture. Although it is the most common metal in the Earth crust, a correlation has been demonstrated between its presence and various pathologies, even serious ones, especially of a neurological type. However, there is a histological gap regarding the role Al can have in contact with the covering and secreting epithelia. The alterations of the ventral and dorsal foot mucocytes and their secretions of the snail Eobania vermiculata caused by Al were investigated in situ by histochemical and lectin‐histochemical techniques. Administration to different experimental groups took place for 3 and 9 days with 50 and 200 μM of AlCl3. Several types of mucocytes were detected with a prevalent secretion of acid glycans in the foot of E. vermiculata. Sulfated glycans prevail in the dorsal region, with one type showing only fucosylated residues and another also having galactosaminylated and glycosaminylated residues. Carboxylated glycans prevail in the ventral region, with presence of galactosaminylated, glycosaminylated, and fucosylated residuals in both cells. Snails treated presented a general decrease of mucin amount in the secreting cells and affected the mucus composition. These changes could alter the rheological and functional properties of the mucus with possible implications for the health of the treated animals.Research Highlights Snails were fed with Al‐contaminated lettuce at different concentrations. In the foot mucocytes produced mucus with prevailing acidic glycans. In the treated resulted a reduction in the amount of mucus and an alteration of glycan composition.

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Glycopattern analysis and structure of the egg extra-cellular matrix in the Apennine yellow-bellied toad, <i>Bombina pachypus (Anura: Bombinatoridae)</i>

Cited by 13 publications

References 46 publications

Histochemical and structural characterization of egg extra-cellular matrix in bufonid toads,Bufo bufoandBufotes balearicus: Molecular diversity versus morphological uniformity

Histochemical and structural characterization of egg extra-cellular matrix in bufonid toads,Bufo bufoandBufotes balearicus: Molecular diversity versus morphological uniformity

Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)

Aluminum exposure alters the pedal mucous secretions of the chocolate‐band snail, Eobania vermiculata (Gastropoda: Helicidae)

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