Glycolytic Activities in the Larval Digestive Tract of Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

Abstract

The larvae of the Japanese horned beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae: Dynastinae), are an example of a saprophage insect. Generally, Scarabaeid larvae, such as T. dichotomus, eat dead plant matter that has been broken down by fungi, such as Basidiomycota. It is thought that β-1,3-glucan, a constituent polysaccharide in microbes, is abundant in decayed plant matter. Studies of the degradation mechanism of β-1,3-glucan under these circumstances are lacking. In the current study, we sought to clarify the relationship between the capacity to degrade polysaccharides and the food habits of the larvae. The total activities and optimum pH levels of several polysaccharide-degrading enzymes from the larvae were investigated. The foregut, midgut and hindgut of final instar larvae were used. Enzymatic activities were detected against five polysaccharides (soluble starch, β-1,4-xylan, β-1,3-glucan, pectin and carboxymethyl cellulose) and four glycosides (p-nitrophenyl (PNP)-β-N-acetylglucosaminide, PNP-β-mannoside, PNP-β-glucoside and PNP-β-xyloside). Our results indicate that the digestive tract of the larvae is equipped with a full enzymatic system for degrading β-1,3-glucan and β-1,4-xylan to monomers. This finding elucidates the role of the polysaccharide-digesting enzymes in the larvae, and it is suggested that the larvae use these enzymes to enact their decomposition ability in the forest environment.

Site‐specific profiles of biochemical properties in the larval digestive tract of Japanese rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

“…) and pH 10.7 in Trypoxylus dichotomus (Wada et al . ). These findings suggest that high pH in the midgut significantly contributes to primary degradation of plant matter and subsequent digestion and absorption.…”

“…) and Xylotrupes gideon (Mishra & Sen‐Sarma ); amylase activity in C. zealandica (Biggs & McGregor ) and T. dichotomus (Wada et al . ); xylanase activity in P. marginata (Cazemier et al . ) and T. dichotomus (Wada et al .…”

“…) and T. dichotomus (Wada et al . ); glucanase activity in T. dichotomus (Wada et al . ); and β‐glucosidase in O. nasicornis (Bayon ), P. marginata (Cazemier et al .…”

“…); glucanase activity in T. dichotomus (Wada et al . ); and β‐glucosidase in O. nasicornis (Bayon ), P. marginata (Cazemier et al . ), T. dichotomus (Wada et al .…”

“…), T. dichotomus (Wada et al . ) and X. gideon (Mishra & Sen‐Sarma ). Production of these organic acids in the digestive tract of scarabaeid larvae has been reported: acetic acid in P. marginata (Cazemier et al .…”