Glycolipids of murine lymphocyte subpopulations. Structural characterization of thymus gangliosides.

Schwarting, Gerald A.; Gajewski, Anna

doi:10.1016/s0021-9258(20)81979-6

Cited by 33 publications

(10 citation statements)

References 34 publications

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“…Although Schwarting and Gajewski (1983) reported the presence of O-acetylated gangliosides in murine thymocytes and T-lymphocytic cells, we could not detect these glycolipids. This is consistent with the observation of Yohe et al (1988), who also failed to find these ganglioside species.…”

Section: Discussioncontrasting

confidence: 88%

Glycolipid changes in murine myelogenous leukemias: neutral glycolipids as markers for specific populations of leukemias

Ariga

Yoshida²,

Nemoto³

et al. 1991

Biochemistry

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We have studied the glycolipid composition of six different murine myelogenous leukemias as well as that of T-cell leukemias and normal spleen cells. Neutral and acidic lipid fractions were isolated by column chromatography on DEAE-Sephadex and analyzed by high-performance thin-layer chromatography (HPTLC) and an HPTLC overlay method. Murine myelogenous leukemias were found to contain globo- and ganglio-series neutral glycolipids, e.g., glucosylceramide (Glc-cer), lactosylceramide (Lac-cer), globotriaosylceramide (Gb3), globoside (Gb4), Forssman glycolipid (Gb5), and asialo-GM1 (GA1). Monoblastic leukemia cells contained increased proportions of Gb3, Gb4, Gb5, and GA1. Monocytic and myelomonocytic leukemia cells contained increased proportions of Glc-cer and Lac-cer. Especially, Glc-cer accounted for approximately 60% of the total neutral glycolipids in monocytic leukemia cells. Gb3 was the major neutral glycolipid in reticulum cell neoplasm type A, and it accounted for approximately 75% of the neutral glycolipids. GA1 was the major neutral glycolipid in myeloblastic and granulocytic leukemia cells as well as T-cell leukemias. Especially, granulocytic leukemia cells contained predominantly GA1, and it accounted for approximately 80% of the total neutral glycolipids. The pattern of gangliosides in myelogenous leukemias was more complex when compared with that of the neutral glycolipids; murine myelogenous leukemias contained at least 13 gangliosides, including such major gangliosides as GM1, GM1b containing N-acetyl neuraminic acid and N-glycolyl neuraminic acid, and Ga1NAc-GM1b. Alterations of glycolipid composition in murine myeloid leukemias may be associated with cellular differentiation and maturation, and therefore these characteristic glycolipid species may be regarded as markers for specific populations of leukemia cells.

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Section: Discussioncontrasting

confidence: 88%

Glycolipid changes in murine myelogenous leukemias: neutral glycolipids as markers for specific populations of leukemias

Ariga

Yoshida²,

Nemoto³

et al. 1991

Biochemistry

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“…Thus, these masking 9-O-acetyl groups must represent a small percentage of the total 9-O-acetylated sialie acids on splenic lymphocytes in general. In keeping with this, it has recently been shown that T ceils express a variety of O-acetylated gangliosides, including 9-O-acetyl-GD3 (18,19,33). These would be detected by CHE-FcD, but not by CD22#Rg.…”

Section: Cd2213 Ligands Are Regionally Expressed On Marginal Zone Lymphocytes Of Spleen and Are Enhanced By Removal Of 9-o-acetyl Estersmentioning

confidence: 75%

Natural ligands of the B cell adhesion molecule CD22 beta can be masked by 9-O-acetylation of sialic acids.

Sjoberg

Powell

Klein

et al. 1994

The Journal of Cell Biology

177

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Abstract. CD22/3 is a B cell-restricted phosphoprotein expressed on the surface of mature resting B cells. It mediates interactions with other cells partly or exclusively via recognition of ot2-6-1inked sialic acids on glycoconjugates. The sialylated N-linked oligosaccharides recognized best by CD22/~ are common to many glycoproteins, suggesting that additional regulatory mechanisms may exist. Since the exocyclic side chain of sialic acid is required for recognition, we explored the effects of a naturally occurring modification of the side chain, 9-O-acetylation. Semisynthetic N-linked oligosaccharides terminating with 9-0-

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“…Rosenfelder et al (1979) first described differing ganglioside patterns of metabolically labeled murine T and B lymphoblasts. Studies on murine thymus gangliosides by Schwarting and Gajewski (1983) indicated the presence of GMib-type gangliosides. In the murine immune system, GMib and GalNAc-GMib were found in spleen (Nakamura et al, 1984(Nakamura et al, ,1987, splenic T lymphoblasts (Miithing et al, 1987), and thymocytes (Miithing et al, 1989).…”

Section: Resultsmentioning

confidence: 99%

“…In the past, GSLs of murine immune cells (Stein et al, 1978) have been analyzed with the aim of detecting GSLs which might be speciFically expressed by, e.g., thymocytes (Schwarting & Gajewski, 1983), macrophages (Mercurio et al, 1984;Yohe et al, 1985;Yohe & Ryan, 1986) and B lymphocytes (Yohe et al, 1990;Chaby et al, 1986). Gangliosides of the Gm^pathway (i.e., Gmis.…”

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confidence: 99%

Structural characterization of gangliosides from resting and endotoxin-stimulated murine B lymphocytes

Pörtner

Peter‐Katalinić²,

Brade³

et al. 1993

Biochemistry

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B lymphocytes from CBA/J mice were stimulated in splenocyte cultures for 72 h with various endotoxins. Bisphosphoryl lipid A from Escherichia coli had the highest stimulatory effect followed by LPS of Citrobacter freundii and Salmonella minnesota as measured by [3H]thymidine uptake. Gangliosides of stimulated B cells (metabolically labeled with D-[1-14C]galactose and D-[1-14C]glucosamine) and unlabeled gangliosides from resting B cells (prepared from spleens without stimulus) were analyzed by high-performance TLC, DEAE anion-exchange HPLC, and immunostaining procedures. Contents of ganglioside-derived sialic acids, quantified by HPLC as their fluorescent derivatives, decreased from stimulated to resident B lymphocytes in the following order: LPS S. minnesota > LPS C. freundii > bisphosphoryl lipid A E. coli > resting B cells. Gangliosides of resting B cells contained more N-glycolyl- than N-acetylneuraminic acid, whereas inverse ratios were found in activated cells, indicating a shift from N-glycolyl- to N-acetylneuraminic acid due to stimulation. Furthermore, a higher disialoganglioside content was characteristic for activated B cells. Fast atom bombardment mass spectrometry was performed with permethylated mono- and disialoganglioside fractions of LPS S. minnesota and LPS C. freundii stimulated B cells. Major gangliosides were GM1a and GD1a beside minute amounts of GD1b. The structural heterogeneity in the gangliosides was caused by (a) N-substitution of the sialic acids with either acetyl or glycolyl groups, (b) variation in the long-chain base (sphingosine, sphinganine), and (c) substitution of the ceramide moiety by fatty acids of different chain length and degree of unsaturation (C16:0, C24:0,24:1).2+ p6

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Glycolipids of murine lymphocyte subpopulations. Structural characterization of thymus gangliosides.

Cited by 33 publications

References 34 publications

Glycolipid changes in murine myelogenous leukemias: neutral glycolipids as markers for specific populations of leukemias

Glycolipid changes in murine myelogenous leukemias: neutral glycolipids as markers for specific populations of leukemias

Natural ligands of the B cell adhesion molecule CD22 beta can be masked by 9-O-acetylation of sialic acids.

Structural characterization of gangliosides from resting and endotoxin-stimulated murine B lymphocytes

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