Glycolipids of a human glioma cell line bearing receptors for platelet‐derived growth factor (PDGF)

Yates, Allan J.; Agudelo, Javier D.; Sung, Ching-Ching

doi:10.1007/bf02536482

Cited by 13 publications

(8 citation statements)

References 25 publications

(11 reference statements)

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“…We previously established a modified tetracyclineregulatable (Tet On) system controlling GD3 synthase gene expression [10] in the GD3-deficient glioma cell line U-1242 MG [11]. We found that the transfected cell line, U1242MG-GD3, responds to doxycycline by increasing its synthesis of GD3.…”

Section: Introductionmentioning

confidence: 98%

Molecular Mechanisms of GD3-Induced Apoptosis in U-1242 MG Glioma Cells

2006

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An increasing amount of evidence indicates that the disialoganglioside GD3 is involved in apoptosis in many cell lines. Our previous studies demonstrated that endogenous GD3 expression induced apoptosis in U-1242 MG glioma cells transfected with the GD3 synthase gene (U1242MG-GD3 cells). In this paper, we present further investigations on the molecular mechanisms of GD3-induced apoptosis in this cell line. We found that endogenously synthesized GD3 localizes to the caveolae of this cell line, where it promotes the localization of death receptor 5 (DR5), tumor necrosis factor receptor-1 (TNF-R1), and Fas (Apo-1) to the caveolae. In addition, caspase-8 was translocated to the caveolar fraction and cleaved; the cleaved proteins were then re-located into the high density fractions. However, GD3 had no effect on the distribution of the adapter protein Fas-associated death domain (FADD). We conclude that GD3 functions as a regulatory molecule early in the extrinsic apoptosis pathway.

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Section: Introductionmentioning

confidence: 98%

Molecular Mechanisms of GD3-Induced Apoptosis in U-1242 MG Glioma Cells

2006

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“…The most complex form of glycosphingolipids are gangliosides, which are characterized by a variable number of negatively charged sialic acid residues attached to hydrophilic sugar moieties. Gangliosides have been shown to play roles in a number of cellular functions, including cell recognition and adhesion (1-3), signal transduction (3), and cell growth regulation (4,5). Although a minor component in most cells, they constitute up to 10% of the total lipid mass in nerve cells (6).…”

Section: Introductionmentioning

confidence: 99%

Number of Sialic Acid Residues in Ganglioside Headgroup Affects Interactions with Neighboring Lipids

Frey

Lee

2013

Biophysical Journal

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Monolayers of binary mixtures of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and asialo-(GA1), disialo-(GD1b) and trisialo-(GT1b) gangliosides were used to determine the effect of ganglioside headgroup charge and geometry on its interactions with the neighboring zwitterionic lipid. Surface pressure versus molecular area isotherm measurements along with concurrent fluorescence microscopy of the monolayers at the air-water interface were complemented with atomic force microscopy imaging of monolayers deposited on solid substrates. Results were used to further develop a proposed geometric packing model that the complementary geometry of DPPC and monosialoganglioside GM1 headgroups affects their close molecular packing, inducing condensation of the layer at small mol % of ganglioside. For GA1, GD1b, and GT1b, a similar condensing effect, followed by a fluidizing effect is seen that varies with glycosphingolipid concentration, but results do not directly follow from geometric arguments because less DPPC is needed to condense ganglioside molecules with larger cross-sectional areas. The variations in critical packing mole ratios can be explained by global effects of headgroup charge and resultant dipole moments within the monolayer. Atomic force microscopy micrographs further support the model of ganglioside-induced DPPC condensation with condensed domains composed of a striped phase of condensed DPPC and DPPC/ganglioside geometrically packed complexes at low concentrations.

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“…The most complex form of glycolipids are gangliosides, which contain one or more negatively charged sialic acid groups. Gangliosides are thought to play roles in a number of cellular functions, including cell recognition and adhesion (1)(2)(3), signal transduction (3), and cell growth regulation (4). Although a minor component in most cells, they constitute 5-10% of the total lipid mass in nerve cells (5), and because they reside primarily on the outer leaflet of the cell membrane, the external surfaces of certain cells contain 10-20 mol % ganglioside.…”

Section: Introductionmentioning

confidence: 99%

Condensing and Fluidizing Effects of Ganglioside GM1 on Phospholipid Films

Frey

Arratia

et al. 2008

Biophysical Journal

120

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Mixed monolayers of the ganglioside G(M1) and the lipid dipalmitoylphosphatidlycholine (DPPC) at air-water and solid-air interfaces were investigated using various biophysical techniques to ascertain the location and phase behavior of the ganglioside molecules in a mixed membrane. The effects induced by G(M1) on the mean molecular area of the binary mixtures and the phase behavior of DPPC were followed for G(M1) concentrations ranging from 5 to 70 mol %. Surface pressure isotherms and fluorescence microscopy imaging of domain formation indicate that at low concentrations of G(M1) (<25 mol %), the monolayer becomes continually more condensed than DPPC upon further addition of ganglioside. At higher G(M1) concentrations (>25 mol %), the mixed monolayer becomes more expanded or fluid-like. After deposition onto a solid substrate, atomic force microscopy imaging of these lipid monolayers showed that G(M1) and DPPC pack cooperatively in the condensed phase domain to form geometrically packed complexes that are more ordered than either individual component as evidenced by a more extended total height of the complex arising from a well-packed hydrocarbon tail region. Grazing incidence x-ray diffraction on the DPPC/G(M1) binary mixture provides evidence that ordering can emerge when two otherwise fluid components are mixed together. The addition of G(M1) to DPPC gives rise to a unit cell that differs from that of a pure DPPC monolayer. To determine the region of the G(M1) molecule that interacts with the DPPC molecule and causes condensation and subsequent expansion of the monolayer, surface pressure isotherms were obtained with molecules modeling the backbone or headgroup portions of the G(M1) molecule. The observed concentration-dependent condensing and fluidizing effects are specific to the rigid, sugar headgroup portion of the G(M1) molecule.

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Glycolipids of a human glioma cell line bearing receptors for platelet‐derived growth factor (PDGF)

Cited by 13 publications

References 25 publications

Molecular Mechanisms of GD3-Induced Apoptosis in U-1242 MG Glioma Cells

Molecular Mechanisms of GD3-Induced Apoptosis in U-1242 MG Glioma Cells

Number of Sialic Acid Residues in Ganglioside Headgroup Affects Interactions with Neighboring Lipids

Condensing and Fluidizing Effects of Ganglioside GM1 on Phospholipid Films

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