Glycolipid patterns during <i>xenopus</i> embryo development

Gornati, Rosalba

doi:10.1006/cbir.1995.1061

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…Up-regulation of oligosialylation along zebrafish development is in agreement with previous observations made on Xenopus laevis showing by in vitro assays that ST8Sia I (SAT-2) and ST8Sia V (SAT-4) activities were dramatically increased along the early development, with a maximum activity at day 4 [47]. However, whereas quantities of both neutral glycolipids and gangliosides sharply increase in X. laevis, only gangliosides appear to be up-regulated in D. rerio [48]. In contrast to glycolipids, evolution of glycoproteins associated α2,8-sialylation does not correlate with the temporal expression and the general increase of mRNAs of ST8Sia II, ST8Sia IV and ST8Sia VI from 10 hpf along the early stages of zebrafish development, therefore implicating a different kind of regulation.…”

Section: Discussionsupporting

confidence: 92%

Developmental regulation of oligosialylation in zebrafish

et al. 2008

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Zebrafish appears as a relevant model for the functional study of glycoconjugates along vertebrate's development. Indeed, as a prelude to such studies, we have previously identified a vast array of potentially stage-specific glycoconjugates, which structures are reminiscent of glycosylation pathways common to all vertebrates. In the present study, we have focused on the identification and regulation of major protein and lipids associated alpha2-8-linked oligosialic acids motifs in the early development of zebrafish. By a combination of partial hydrolysis, anion exchange HPLC-FD and mass spectrometry, we demonstrated that glycoproteins and glycolipids differed by the extent and the nature of their substituting oligosialylated sequences. Furthermore, relative quantifications showed that alpha2-8-linked sialylation was differentially regulated in both families of glycoconjugates along development. Accordingly, we established that alpha2,8-sialyltransferase mRNA levels was directly correlated with changes of alpha2,8-sialylation status of glycolipids, but independent of those observed on major glycoproteins that appear to originate from the mother.

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Section: Discussionsupporting

confidence: 92%

Developmental regulation of oligosialylation in zebrafish

et al. 2008

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“…Our results are in keeping with the ones reported by Gornati et al (1995Gornati et al ( , 1997 concerning the in vitro activities of sialidases and glycosyltransferases during the early development of X. laevis. They found that GD3 was the most represented ganglioside during early embryogenesis and that SAT-2 (GD 3 synthase) had an activity peak at day 4 of development (Gornati et al, 1997).…”

Section: Xlcgt and Xlgd 3 Synthase Temporal And Spatial Expression Pasupporting

confidence: 94%

“…The activities of glycosyltransferases and sialidases during the early development of Xenopus, analyzed by Gornati et al (1995Gornati et al ( ,1997, showed the highest enzymatic activity during early embryogenesis. Recently, Rimoldi et al (2007) reported the sequences of two another isoforms of ST8Sia I and their expression during later Xenopus embryogenesis, probably reflecting the pseudotetraploidy in Xenopus laevis.…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibianXenopus laevis

Luque¹,

Crespo²,

Mónaco³

et al. 2007

Developmental Dynamics

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Gangliosides are a subfamily of complex glycosphingolipids (GSLs) with important roles in many biological processes. In this study, we report the cDNA cloning, functional characterization, and the spatial and temporal expression of Xlcgt and Xlgd3 synthase during Xenopus laevis development. Xlcgt was expressed both maternally and zigotically persisting at least until stage 35. Maternal Xlgd3 synthase mRNA could not be detected and showed a steady-state expression from gastrula to late tailbud stage. Xlcgt is mainly present in involuted paraxial mesoderm, neural folds, and their derivatives. Xlgd3 synthase transcripts were detected in the dorsal blastoporal lip, in the presumptive neuroectoderm, and later in the head region, branchial arches, otic and optic primordia. We determined the effect of glycosphingolipid depletion with 1-phenyl-2-palmitoyl-3-morpholino-1-propanol (PPMP) in mesodermal layer. PPMP-injected embryos showed altered expression domains in the mesodermal markers. Our results suggest that GSL are involved in convergent-extension movements during early development in Xenopus. Developmental Dynamics 237: 112-123, 2008.

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“…There is increasing evidence that GSL and especially gangliosides are expressed during amphibian development and may be involved in the cellular events that take place during development (Turner et al, 1992;Gornati et al, 1995;Kubo et al, 1995). In 1974, Stearns suggested that yolk platelets provide the organic components used during segmentation and in cell-cell recognition during gastrulation (Stearns, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…In 1991, Hidari et al determined the composition of acidic glycolipids in the oocyte of the amphibian Xenopus. More recently, Gornati et al (1995) established the pattern of both neutral glycosphingolipids and gangliosides during the first 6 days of Xenopus laevis development. Moreover, a neutral glycolipid was found to be involved in a novel cellular adhesion mechanism.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the synthesis of glycosphingolipid by a ceramide analogue (PPMP) in the gastrulation of Bufo arenarum

Aybar

Fuentes

Sánchez

2000

Zygote

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In the present study the role of glycosphingolipids (GSL) in amphibian development was investigated. We analysed the de novo synthesis of neutral GSL and gangliosides through the initial stages of Bufo arenarum embryo development and their participation during gastrulation using 1-phenyl-2-palmitoyl-3-morpholino-1-propanol (PPMP), a potent inhibitor of glucosylceramide synthase. Ganglioside synthesis began at the blastula stage and reached a maximum during gastrulation (stages 10-12) while neutral GSL synthesis showed a slight gradual increase, the former being quantitatively more significant than the latter. Ganglioside synthesis was reduced by 90% while neutral GSL synthesis was inhibited by 65% when embryos at blastula stage were cultured for 24 h in 20 μM PPMP. The depletion of GSL from amphibian embryos induced an abnormal gastrulation in a dose-dependent manner. We found that PPMP had a pronounced effect on development since no embryos exhibited normal gastrulation; their developmental rate either slowed down or, more often, became totally arrested. Morphological analysis of arrested embryos revealed inhibition of the gastrulation morphogenetic movements. Analysis of mesodermal cell morphology in those embryos showed a severe decrease in the number and complexity of cellular extensions such as filopodia and lamellipodia. Mesodermal cells isolated from PPMP-treated embryos had very low adhesion percentages. Our results suggest that glycosphingolipids participate in Bufo arenarum gastrulation, probably through their involvement in cell adhesion events.

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Glycolipid patterns during xenopus embryo development

Cited by 14 publications

References 10 publications

Developmental regulation of oligosialylation in zebrafish

Developmental regulation of oligosialylation in zebrafish

Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibianXenopus laevis

Inhibition of the synthesis of glycosphingolipid by a ceramide analogue (PPMP) in the gastrulation of Bufo arenarum

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