Glycohistochemistry of the <i>Tilapia</i> spp. stomach

Scocco, Paola; Ceccarelli, Piero; Menghi, G

doi:10.1111/j.1095-8649.1996.tb00056.x

Cited by 44 publications

(43 citation statements)

References 28 publications

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“…Os testes revelaram a presença de GPs neutras e ácidas no terço superior das células cilíndricas superficiais. Estas GPs, produzidas pelas células epiteliais, podem proteger a mucosa do ácido contido no estômago (SCOCCO et al, 1996;MORRISON & WRIGHT-JR., 1999).…”

Section: Discussionunclassified

Histologia e caracterização histoquímica do tubo gastrintestinal de Pimelodus maculatus (Pimelodidae, Siluriformes) no reservatório de Funil, Rio de Janeiro, Brasil

Santos

Duarte

Souza

et al. 2007

Iheringia, Sér. Zool.

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ABSTRACT. Histology and histochemical characterization of the digestive tract of Pimelodus maculatus (Pimelodidae, Siluriformes) in Funil reservoir, Rio de Janeiro, Brazil. Histological structures of the digestive traits of Pimelodus maculatus La Cepède, 1803, in the Funil reservoir (22º30'-22º35'S; 44º35'-44º40'W) were described using haematoxilin-eosin (HE), trichome Gomori, periodic acid Schiff (PAS) and alcian blue (AB) pH 2.5 techniques. The main aim of this study was to detect the levels of glycoproteins (GPs) in the mucous tunic based in histochemical techniques and to characterize eventual alteration in the digestive tract of this species in a polluted system. Fifty-four adult individuals collected between April-2003 and July-2004 (TL=19-38 cm) were examined. The mucosa showed longitudinal folds in the buccopharyngeal cavity, with a stratified squamous epithelium. The oesophagus showed epithelium similar to buccopharyngeal cavity, with a predominance of mucous cells, which reacted strongly with the used methods. The stomach showed three regions: cardic, fundic and pyloric. The mucous tunic is continuously revested by a single cylindrical epithelium which showed positive reaction to AB and PAS only at the apical cells surface. The proper tunic has tubular glands that ramify gradatively, being a cellular type named oxyntopeptic, related to chloridric acid and pepsinogenic cells synthesis. The intestine showed vilosities, with the mucous tunic revested by a single cylindrical epithelium with striated planure and caliciform cells. The duodenum submucosa layer was located at the coledocum opening, as a single cylindrical epithelium lacking caliciforms cells, reacting positively with both PAS and AB. The eutrophic environment of Funil reservoir did not seem to influence morphologic patterns of the structures (oesophagus, stomach and intestine) and no modification was observed in the mucous cells activity. KEYWORDS.Morphology, glycoproteins, fishes, Pimelodidae. RESUMO. As estruturas teciduais do trato gastrintestinal dePimelodus maculatus La Cepède, 1803, do reservatório de Funil (22º30'-22º35'S; 44º35'-44º40'W) foram descritas através das técnicas de hematoxilina-eosina (HE), tricômico Gomori, ácido Periódico de Schiff (PAS) e alcian blue (AB) pH 2,5. Objetivou-se a detecção de glicoproteínas (GPs) na mucosa através de uma análise histoquímica e caracterização de possíveis alterações ao longo do trato digestório destes peixes neste sistema de elevado grau de alteração. Cinqüenta e quatro indivíduos adultos, coletados entre abril de 2003 e julho de 2004 (CT=19-38 cm) foram utilizados. Na cavidade bucofaríngea, a mucosa apresenta-se com pregas longitudinais e o epitélio é do tipo estratificado pavimentoso. O esôfago apresenta o mesmo tipo de epitélio da cavidade bucofaríngea, destacando-se a predominância de células mucosas que tiveram forte reação aos métodos utilizados. O estômago foi diferenciado em região cárdica, fúndica e pilórica. A mucosa é continuamente revestida por um epitélio simples cilíndrico que...

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Section: Discussionunclassified

Histologia e caracterização histoquímica do tubo gastrintestinal de Pimelodus maculatus (Pimelodidae, Siluriformes) no reservatório de Funil, Rio de Janeiro, Brasil

Santos

Duarte

Souza

et al. 2007

Iheringia, Sér. Zool.

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“…The aim of the present study was to take a multidisciplinary approach and to integrate the expertise of histochemists (Scocco et al, 1996(Scocco et al, , 1997(Scocco et al, , 1998 with that of biochemists (Ius et al, 1993). This was in order to directly visualize the phthalate esters occurrence in the alimentary canal of a polyhybrid of Tilapia spp.…”

Section: Introductionmentioning

confidence: 99%

Immnunohistochemical detection of phthalate esters in the alimentary canal of Tilapia spp.

Menghi

Sabbieti

Menghi

et al. 2002

Journal of Fish Biology

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An examination of the occurrence and distribution of phthalate esters in the alimentary canal of a polyhybrid of Tilapia gave evidence of different and selective patterns of distribution in the organ tissues: the phthalate esters were shown to be concentrated in the stomach and anterior intestine. The restricted distribution of phthalate esters can have implications for the physiology of the digestive system. The phthalates, stored in the oxyntic cells of the gastric tubular glands, probably interfere with the digestive process. The strategic location of the enterocytes in the anterior intestine implies that they can hamper the reabsorption of digestion products. The endocrine disrupting effects known for these chemicals are probably related to the absorption of them via the alimentary canal.

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“…This corroborates other researches indicating that in some fish species digestion starts in the oesophagus and continues in the stomach (DOMENEGHINI et al, 1998). Likewise, the high level of histochemical complexity in the oesophagus could be related to the absence of salivary glands (SCOCCO et al, 1996). It's important to notice that digestion in mammals starts in the mouth, due to the presence of secretion from the salivary glands.…”

Section: Glicoconjugates In the Silverside Odontesthes Bonariensismentioning

confidence: 99%

Characterization of glycoconjugates in the pharyngeal cavity and the oesophagus of Odontesthes bonariensis by lectins

et al. 2012

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ResumoCaracterização de glicoconjugados da cavidade faríngea e esôfago de Odontesthes bonariensis por lectinas. Sete tipos de lectinas biotiniladas foram usados para examinar padrões de expressão de glicoconjugados (GCs) da mucosa da cavidade faríngea e esôfago do Odontesthes bonariensis. Sítios de ligação para Con-A, RCA-I e WGA foram positivos para o glicocálix, células epiteliais e células mucosas em ambos os órgãos com padrões de reação similar. PNA apenas marcou o glicocálix e as células epiteliais da mucosa em ambos os órgãos. DBA, SBA e UEA-I marcaram o esôfago exclusivamente e uma maior afinidade com o glicocálix e as células epiteliais foi observada. Os resultados deste estudo foram discutidos esuas possíveis implicações funcionais foram comparadas. Palavras-chave:Cavidade da faringe; Esôfago; Glicoconjugados; Histoquímica com lectinas; 'Silverside' AbstractSeven types of biotinylated lectins were used to examine glycoconjugates (GCs) expression patterns in the mucosa of the pharyngeal cavity and the oesophagus of Odontesthes bonariensis. Con-A, RCA-I, and WGA binding sites were positive for glycocalyx, epithelial cells, and mucous cells in both organs with similar reaction patterns. PNA just stained glycocalyx and epithelial cells in the mucosa in both organs. DBA, SBA, and UEA-I stained only the oesophagus, and a greater affinity to glycocalyx and the epithelial cells was observed. The results of this study were discussed and their possible functional implications were compared.

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Glycohistochemistry of the Tilapia spp. stomach

Cited by 44 publications

References 28 publications

Histologia e caracterização histoquímica do tubo gastrintestinal de Pimelodus maculatus (Pimelodidae, Siluriformes) no reservatório de Funil, Rio de Janeiro, Brasil

Histologia e caracterização histoquímica do tubo gastrintestinal de Pimelodus maculatus (Pimelodidae, Siluriformes) no reservatório de Funil, Rio de Janeiro, Brasil

Immnunohistochemical detection of phthalate esters in the alimentary canal of Tilapia spp.

Characterization of glycoconjugates in the pharyngeal cavity and the oesophagus of Odontesthes bonariensis by lectins

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