Glycohaemoglobin: Comparison of 12 Analytical Methods, Applied to Lyophilized Haemolysates by 101 Laboratories in an External Quality Assurance Programme

Weykamp, C.W.; Tj, Penders; Muskiet, Frits A. J.; Vanderslik, W

doi:10.1177/000456329303000210

Cited by 22 publications

(15 citation statements)

References 8 publications

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“…The results obtained in the present study are similar to those found in other surveys of GHb (9,10,13). A survey of the American College of Pathologists in 1990 reported interlaboratory CV that ranged from 3.4% for HPLC to 17.2% for one type of HbA x assay by ion exchange chromatography (10).…”

Section: Conclusion-supporting

confidence: 89%

“…Despite the theoretical nonlinear relationship between HbA lc and total GHb, this use of calibrators resulted in low long-term imprecision and comparability between different assay methods. Using this approach, similar improvements have also been demonstrated in Europe by Weykamp and colleagues (9,24) and the feasibility of this method has recently been shown in Australia (25).…”

Section: Conclusion-mentioning

confidence: 52%

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Interlaboratory Variation of GHb Assays in Victoria, Australia

et al. 1996

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Section: Conclusion-supporting

confidence: 89%

Section: Conclusion-mentioning

confidence: 52%

Interlaboratory Variation of GHb Assays in Victoria, Australia

et al. 1996

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“…Georgopoulos and Phair studied only adults with DM1 in poor control (HbA 1 12.8 Ϯ 0.6%; normal range: 3.9 -7.7%) (12), whereas in our study DM1 patients were in fair or poor control (mean HbA 1c 9.2 Ϯ 1.9%; normal range: 4.6 -6.1%). Comparison of metabolic regulation (HbA 1c ) between the two studies is difficult however, because in the first study HbA 1 percentages were presented instead of HbA 1c , and HbA 1 was measured by agarose gel electrophoresis rather than by HPLC (44). Another explanation for the difference in CM clearance rate could be the age dependency of CM clearance.…”

Section: Discussionmentioning

confidence: 99%

Postprandial Chylomicron Clearance Rate in Late Teenagers with Diabetes Mellitus Type 1

Waarde¹,

Odink²,

Rouwé³

et al. 2001

Pediatr Res

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A delayed chylomicron (CM) clearance rate, a known risk factor for atherosclerosis, has been described in adults with diabetes type 1 (DM1). We determined the CM clearance rate in late teenagers with DM1, and the relationship between CM clearance rate and elevated plasma lipid concentrations in DM1 teenagers in poor metabolic control (as characterized by HbA 1c percentage). Plasma lipids and CM clearance were determined in nine patients with DM1 (mean age Ϯ SD: 17.5 Ϯ 0.6 y) and four healthy controls (mean age Ϯ SD: 20.1 Ϯ 0.8 y), by measuring breath 13 CO 2 , plasma triglyceride, retinyl palmitate, and 13 Clabeled oleic acid concentrations, after oral administration of a fat-rich meal together with vitamin A and 13 C-oleic acid. In patients with DM1, fasting triglyceride and cholesterol concentrations were positively correlated with HbA 1c percentage (p Ͻ 0.05). Neither in DM1 patients, nor in controls, was an elevated triglyceride concentration (above 1.7 mmol/L) found. Yet, in 22% of DM1 patients, cholesterol concentration was above 5.2 mmol/L, but not in any of the controls. CM clearance rate in DM1 patients was similar to that in controls and did not signif- Diabetes mellitus type 1 (DM1) is associated with a 2-4 fold increased risk for cardiovascular disease (1). Specific diabetesrelated phenomena have been suggested to play a role in the observed association, such as glycosylation of proteins (2), presence of renal disease (3), elevated concentrations of plasma cholesterol, triglycerides, and low density lipoprotein (LDL) cholesterol (4), and altered composition of very low density lipoprotein (VLDL) and LDL (5, 6). In children and late teenagers, studies on risk factors associated with DM1 and atherosclerosis have mainly focused on cholesterol and triglyceride concentrations (7-9). Elevated concentrations of both cholesterol and triglycerides have been reported in children with DM1 in poor metabolic control, which tended to decrease to nondiabetic values upon improvement of metabolic control (7-9).A delayed chylomicron (CM) clearance has been identified as a risk factor for atherosclerosis in patients with coronary heart disease, in patients with type 2 diabetes, and in adult patients with DM1 (10 -12). Insulin enhances the metabolism of CM by stimulating the hydrolysis of CM triglycerides by lipoprotein lipase and the hepatic uptake of CM remnants (11). A relative deficiency of insulin, such as present in DM1, could therefore lead to a delay in CM clearance. A delay in CM clearance would imply a prolonged postprandial hyperlipidemia, which supposedly leads to increased deposition of CM contents into the arterial wall and to an unfavorable translocation of cholesteryl-esters from HDL to CM (13,14). Delays in CM clearance are amenable to dietary intervention, by changing the intake of fish oil (long chain PUFA) (15), monounsaturated-fat (16)

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“…Three milliliter of fasting (8 h) venous blood samples were taken from each child participating in the study and divided into two parts: the first part was added to tube containing EDTA for glycosylated hemoglobin determination by cation-exchange resin [16] and the second part was put in a serum separator tube. The separated serum was stored at -201C for determination of E-selectin, total cholesterol, and triglyceride (TG).…”

Section: Participants and Methodsmentioning

confidence: 99%

E-selectin and vascular complications in children with type 1 diabetes mellitus

Wakeel¹,

El-Kassas²,

Amer³

et al. 2014

Medical Research Journal

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Objective The aim of the study was to investigate the relationship between serum E-selectin (as a marker of endothelial activation) and vascular complications in children and adolescents with type 1 diabetes mellitus (T1DM). Participants and methodsThis was a case-control study including 30 children with T1DM (with mean age 10.8 years, disease duration 7.4 years) and 30 matched healthy controls. Serum E-selectin, glycosylated Hb (HbA1c), total cholesterol, and triglycerides (TG) were determined. Blood pressure and anthropometric parameters were also measured.Results Levels of serum E-selectin, cholesterol, and TG in patients were significantly higher than in controls; these levels were also higher in metabolically poorly controlled and albuminuric T1DM patients compared with metabolically well-controlled and nonalbuminuric T1DM patients. There was a positive correlation between serum E-selectin and HbA1c (r = 0.9, P < 0.001), diastolic blood pressure (DBP) Z-score (r = 0.5, P < 0.01), cholesterol (r = 0.7, P < 0.001), TG (r = 0.6, P < 0.001), and duration of diabetes (r = 0.4, P < 0.03). After adjustment for age, HbA 1 C, serum cholesterol, serum TG, BMI Z-score, systolic blood pressure Z-score, DBP Z-score, and duration of diabetes, the strongest significant associations were shown between serum E-selectin and glycosylated Hb (HbA1c).Conclusion We conclude that elevated biological markers of endothelial cell activation, such as serum E-selectin, may be related to vascular complications of DM, such as diastolic hypertension and microalbuminuria, in children with type 1 diabetes.

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Glycohaemoglobin: Comparison of 12 Analytical Methods, Applied to Lyophilized Haemolysates by 101 Laboratories in an External Quality Assurance Programme

Cited by 22 publications

References 8 publications

Interlaboratory Variation of GHb Assays in Victoria, Australia

Interlaboratory Variation of GHb Assays in Victoria, Australia

Postprandial Chylomicron Clearance Rate in Late Teenagers with Diabetes Mellitus Type 1

E-selectin and vascular complications in children with type 1 diabetes mellitus

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