Glycogen depletion in intrafusal fibres in rats during short‐duration high‐intensity treadmill running

Yoshimura, Atsushi; Toyoda, Yuko; Murakami, Taro; Yoshizato, Hideo; Ando, Yumiko; Fujitsuka, Noriaki

doi:10.1111/j.1365-201x.2005.01455.x

Cited by 9 publications

(7 citation statements)

References 28 publications

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“…Midventricular sections were stained with Schiff reagent (Polyscientific, Bay Shore, NY) to identify glycogen in the hearts (periodic acidSchiff (PAS) staining) (28) or by Masson's Trichrome method to identify collagen for fibrosis (20).…”

Section: Methodsmentioning

confidence: 99%

Cardiomyocyte Specific Deficiency of Serine Palmitoyltransferase Subunit 2 Reduces Ceramide but Leads to Cardiac Dysfunction

Lee

Kim

et al. 2012

Journal of Biological Chemistry

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Background:The importance of de novo ceramide biosynthesis in maintaining cardiac function is unknown. Results: Deletion of serine palmitoyltransferase subunit Sptlc2 reduced cardiac ceramide and caused cardiac dysfunction associated with activation of ER stress. Conclusion: Reduced ceramide content by Sptlc2 deficiency does not protect against lipid toxicity associated with increased saturated acyl CoAs. Significance: Development of disease by lipotoxicity is caused by a number of changes in lipidome.

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Section: Methodsmentioning

confidence: 99%

Cardiomyocyte Specific Deficiency of Serine Palmitoyltransferase Subunit 2 Reduces Ceramide but Leads to Cardiac Dysfunction

Lee

Kim

et al. 2012

Journal of Biological Chemistry

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“…Midventricular sections were stained with Schiff Reagent (Polyscientific, Bay Shore, NY) to identify glycogen in hearts [periodic acid-Schiff (PAS) staining] (30). The specificity of glycogen staining was confirmed by treating sections with diastase to digest tissue glycogen, followed by regular PAS staining (30). Sectioned heart tissues were stained for DNA fragmentation by a terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining protocol according to the manufacturerʼs instructions (R and D Systems, Minneapolis, MN).…”

Section: Histologymentioning

confidence: 99%

Ceramide is a cardiotoxin in lipotoxic cardiomyopathy

Park

Noh

et al. 2008

Journal of Lipid Research

351

299

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Ceramide is among a number of potential lipotoxic molecules that are thought to modulate cellular energy metabolism. The heart is one of the tissues thought to become dysfunctional due to excess lipid accumulation. Dilated lipotoxic cardiomyopathy, thought to be the result of diabetes and severe obesity, has been modeled in several genetically altered mice, including animals with cardiac-specific overexpression of glycosylphosphatidylinositol (GPI)-anchored human lipoprotein lipase (LpL GPI ). To test whether excess ceramide was implicated in cardiac lipotoxicity, de novo ceramide biosynthesis was inhibited pharmacologically by myriocin and genetically by heterozygous deletion of LCB1, a subunit of serine palmitoyltransferase (SPT). Inhibition of SPT, a rate-limiting enzyme in ceramide biosynthesis, reduced fatty acid and increased glucose oxidation in isolated perfused LpL GPI hearts, improved systolic function, and prolonged survival rates. Our results suggest a critical role for ceramide accumulation in the pathogenesis of lipotoxic cardiomyopathy.-Park, T

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“…Serial sections in the mid-belly of the muscles were prepared for histochemical procedures, myosin ATPase [16], succi-nate dehydrogenase (SDH, [17]), alpha-amylase-periodic acid schiff (PAS, [14]) and IMCL (OR; oil red O staining, [18] [19]. The evaluation of the histochemical profile of each fiber was estimated by optical density based on a computer system [14]. In brief, two criteria, complete transmission without a slide glass (130 ± 0.5) and with one (120 ± 0.5), were used to adjust to constant light absorbance (0 = complete absorbance, 250 = complete transmission).…”

Section: Histochemical Analyses In the Musclementioning

confidence: 99%

“…IMCL of soleus muscle is reportedly higher than that of the other skeletal muscles [2] [13], and IMCL of type I is higher than type II fiber types [8], while soleus muscle is mainly composed of type I fibers [14]. The objective of this study was to examine the effect of exercise training on glycogen and IMCL content of soleus muscle in NASH model rats.…”

Section: Introductionmentioning

confidence: 99%

Effects of Aerobic Exercise on the Intramuscular Lipid and Glycogen Content of Fiber Types in Soleus Muscles of Non-Alcoholic Steatohepatitis Model Rats

Mochizuki¹,

Hayashi²,

Yoshimura³

et al. 2015

OJEMD

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We studied the effects of exercise on muscle mitochondria, and lipid and glycogen content in nonalcoholic steatohepatitis (NASH) model rats. Male Sprague-Dawley rats were randomly separated into 3 groups: the control group was fed standard chow; the NASH group was fed a methioninecholine-deficient high-fat diet (MCD); the NASH-exercise group was fed the MCD and exercised three times a week. Exercise training consisted of continuous running for thirty minutes at a 13 m/min, 6˚ slope on a motor-driven rodent treadmill for 6 weeks. Mitochondria content in NASH group decreased in the both fiber types compared with those of the control group. As compared between the NASH and NASH-exercise groups, however, exercise not only promoted significant improvements in liver fibrosis and cirrhosis and triglyceride (TG) content but also increased mitochondria content in type I muscle fiber in particular. These data suggest that exercise improved hepatic steatosis in NASH model rats and can prevent the progression of NASH.

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Glycogen depletion in intrafusal fibres in rats during short‐duration high‐intensity treadmill running

Abstract: The results suggest that during brief-intensity exercise, as the glycogen content of type IIA fibres is reduced earlier than that of type I fibres, bag2 fibres are most important early in this type of exercise.

Cited by 9 publications

References 28 publications

Cardiomyocyte Specific Deficiency of Serine Palmitoyltransferase Subunit 2 Reduces Ceramide but Leads to Cardiac Dysfunction

Cardiomyocyte Specific Deficiency of Serine Palmitoyltransferase Subunit 2 Reduces Ceramide but Leads to Cardiac Dysfunction

Ceramide is a cardiotoxin in lipotoxic cardiomyopathy

Effects of Aerobic Exercise on the Intramuscular Lipid and Glycogen Content of Fiber Types in Soleus Muscles of Non-Alcoholic Steatohepatitis Model Rats

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