Glycogen catabolism, but not its biosynthesis, affects virulence of Fusarium oxysporum on the plant host

Corral-Ramos, Cristina; Roncero, M. Isabel G.

doi:10.1016/j.fgb.2015.03.014

Cited by 10 publications

(3 citation statements)

References 44 publications

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“…Starch and Sucrose metabolism also include glycogen biosynthesis. Glycogen is an essential glucose (and energy) storage molecule in fungi [43]. Glycogen biosynthesis depends on the catalysis of GYG1 [44], while PYG can catalyze and regulate the decomposition of glycogen to release D-glucose 1-phosphate from its intracellular storage.…”

Section: Discussionmentioning

confidence: 99%

Study on the inhibitory activity and mechanism of Mentha haplocalyx essential oils nanoemulsion against Fusarium oxysporum growth

Liao,

Wen,

Nie

et al. 2024

Preprint

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As a green and environmentally friendly natural product, Mentha haplocalyx essential oil (MEO) inhibits F. oxysporum, but its field application is limited by its poor water solubility. The nanoemulsion can improve bioavailability and provide a green way to prevent and control P. notoginseng root rot. In this study, Tween-80 and anhydrous ethanol were selected as carriers, and the ultrasonic method was adopted to prepare a nanoemulsion of MEO (MNEO) with an average particle size of 26.07 nm. Compared with traditional emulsion of MEO (MTEO, MEO dissolved in an aqueous solution of 2% DMSO and 0.1% Tween 80), MNEO was superior in inhibiting F. oxysporum in terms of spore germination, hyphal growth, and minimum inhibitory concentration (MIC). Through transcriptomic and metabolomic analyses, it was found that both MNEO and MTEO can inhibit the Glycolysis/Gluconeogenesis and Starch and Sucrose metabolism pathways of F. oxysporum. Nevertheless, MNEO can inhibit the expression of more genes and metabolites. In addition, some key genes in meiosis, ribosome, and ribosome biogenesis were also significantly downregulated only under MNEO treatment. These genes and metabolites provide valuable information for the later prevention and control of F. oxysporum and provide a theoretical basis for better utilization of MEO resources to develop plant-derived bacteriostatic agents and agricultural modernization and ecological development.

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Section: Discussionmentioning

confidence: 99%

Study on the inhibitory activity and mechanism of Mentha haplocalyx essential oils nanoemulsion against Fusarium oxysporum growth

Liao,

Wen,

Nie

et al. 2024

Preprint

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“…Many fungal genes have been shown to be essential for full virulence of F. oxysporum including G-protein subunits ( Jain et al, 2002 , 2003 ) and Rho-type GTPase Rho1 ( Martínez-Rocha et al, 2008 ), several protein kinases such as MAP kinases ( Di Pietro et al, 2001 ; Ding et al, 2015 ; Turrà et al, 2015 ), two-component histidine kinase ( Rispail and Di Pietro, 2010 ) and cAMP-dependent protein kinase A ( Kim et al, 2011 ), transcription factors such as REN1, FOW2, SGE1, FTF1, PacC, Ctf1, xlnR, Snt2, fost12, HpaX and Con7-1 ( Caracuel et al, 2003 ; Ohara et al, 2004 ; Imazaki et al, 2007 ; Ramos et al, 2007 ; Calero-Nieto et al, 2007 ; Rocha et al, 2008 ; Michielse et al, 2009b ; Rispail and Di Pietro, 2009 ; Asunción García-Sánchez et al, 2010 ; Denisov et al, 2011 ; López-Berges et al, 2012 ; Ruiz-Roldán et al, 2015 ; Niño-Sánchez et al, 2016 ), F-box protein FRP1 and its interactor CRE ( Duyvesteijn et al, 2005 ; Jonkers and Rep, 2009 ; Jonkers et al, 2011 ), velvet complex ( López-Berges et al, 2013 ), membrane protein Msb2 and Sho1 ( Pérez-Nadales and Di Pietro, 2011 , 2015 ), mitochondrial protein FOW1 ( Inoue et al, 2002 ) and co-chaperone Dnj1 ( Lo Presti et al, 2016 ). In addition, genes encoding for chitin synthases ( Madrid et al, 2003 ; Martín-Urdíroz et al, 2008 ; Martín-Udíroz et al, 2004 ), alcohol dehydrogenase ( Corrales Escobosa et al, 2011 ), pisatin demethylase ( Coleman et al, 2011 ), tomatinase ( Pareja-Jaime et al, 2008 ), glucanosyltransferase ( Caracuel et al, 2005 ), N -acetylglucosamine transferase ( López-Fernández et al, 2013 ), mannosyltransferase ( Li et al, 2014 ), 3-carboxy- cis, cis -muconate lactonizing enzyme ( Michielse et al, 2012 ), and other enzymes involved in arginine biosynthesis ( Namiki et al, 2001 ), glycogen catabolism ( Corral-Ramos and Roncero, 2015 ) were also shown to play roles in virulence of F. oxysporum . Thus, it is clear that F. oxysporum pathogenicity is regulated by a complicated network, which includes signal perception, transduction, gene transcription, and metabolism.…”

Section: Introductionmentioning

confidence: 99%

CCR4-Not Complex Subunit Not2 Plays Critical Roles in Vegetative Growth, Conidiation and Virulence in Watermelon Fusarium Wilt Pathogen Fusarium oxysporum f. sp. niveum

et al. 2016

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CCR4-Not complex is a multifunctional regulator that plays important roles in multiple cellular processes in eukaryotes. In the present study, the biological function of FonNot2, a core subunit of the CCR4-Not complex, was explored in Fusarium oxysporum f. sp. niveum (Fon), the causal agent of watermelon wilt disease. FonNot2 was expressed at higher levels in conidia and germinating conidia and during infection in Fon-inoculated watermelon roots than in mycelia. Targeted disruption of FonNot2 resulted in retarded vegetative growth, reduced conidia production, abnormal conidial morphology, and reduced virulence on watermelon. Scanning electron microscopy observation of infection behaviors and qRT-PCR analysis of in planta fungal growth revealed that the ΔFonNot2 mutant was defective in the ability to penetrate watermelon roots and showed reduced fungal biomass in root and stem of the inoculated plants. Phenotypic and biochemical analyses indicated that the ΔFonNot2 mutant displayed hypersensitivity to cell wall perturbing agents (e.g., Congo Red and Calcofluor White) and oxidative stress (e.g., H2O2 and paraquat), decreased fusaric acid content, and reduced reactive oxygen species (ROS) production during spore germination. Our data demonstrate that FonNot2 plays critical roles in regulating vegetable growth, conidiogenesis and conidia morphology, and virulence on watermelon via modulating cell wall integrity, oxidative stress response, ROS production and FA biosynthesis through the regulation of transcription of genes involved in multiple pathways.

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“…However, 13 genes (50%) were not necessary for pathogenicity, but were previously found to be associated with Phi. Gnn1 is a types of glycogen, which is involved in the pathogenicity of Fusarium oxysporum to plant hosts[21]. The striatal homologue fsr1 forms an intimal-related complex that regulates the virulence of Fusarium oxysporum, a maize pathogen.…”

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confidence: 99%

Deciphering the transcriptomic response of Ilyonectria robusta in relation to Ginsenoside Rg1 treatment and the development of panax ginseng rusted root disease

Li¹,

Zhang²,

Peng³

et al. 2019

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Rust rot is a severe Panax ginseng disease caused by Ilyonectria robusta . The severity of the disease is related to the content of residual ginsenoside content in the soil. Herein, the effects of different concentrations of ginsenoside Rg1 on ginseng rust rot and its pathogens were studied, and the growth and sporulation of fungi cultured in vitro were determined. The results showed that Rg1 significantly promoted the mycelial growth, and sporulation compared to the control, and aggravated the disease symptoms of Panax ginseng . A total of 6,708 transcripts out of 213,131 annotated genes identified from global transcriptomic analysis were differentially expressed in I.robusta grown in the Rg1 treatment. These genes were found to be related to the carbon-nitrogen metabolism, transport, and assimilation. Many of these genes were also associated with pathogenicity based on the Phi-base database. Several transcription factors were related to specific biological processes, such as nitrogen utilization. The current results revelation that Rg1 plays a major role in the development of rust rot by promoting fungal cell growth and affecting the expression of genes required for pathogenesis. Rg1 could aggravate the invasion of I.robusta on ginseng root, which preliminarily revealed the reason for the aggravation of rust rot in ginseng c ontinuous cropping obstacle.

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Glycogen catabolism, but not its biosynthesis, affects virulence of Fusarium oxysporum on the plant host

Cited by 10 publications

References 44 publications

Study on the inhibitory activity and mechanism of Mentha haplocalyx essential oils nanoemulsion against Fusarium oxysporum growth

Study on the inhibitory activity and mechanism of Mentha haplocalyx essential oils nanoemulsion against Fusarium oxysporum growth

CCR4-Not Complex Subunit Not2 Plays Critical Roles in Vegetative Growth, Conidiation and Virulence in Watermelon Fusarium Wilt Pathogen Fusarium oxysporum f. sp. niveum

Deciphering the transcriptomic response of Ilyonectria robusta in relation to Ginsenoside Rg1 treatment and the development of panax ginseng rusted root disease

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