Glycoconjugate synthesis during early pregnancy: Hyaluronate synthesis and function

Carson, Daniel D.; Dutt, Anuradha; Tang, Jy-Ping

doi:10.1016/0012-1606(87)90120-5

Cited by 100 publications

(47 citation statements)

References 15 publications

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“…HA may also have a role in the preparation of the endometrium for embryo implantation. Its level increases significantly on the day of implantation in mouse uterus, particularly in regions that contain stromal cells proliferating in preparation for embryo implantation [2]. Based on these data, HETM became a candidate for improving implantation rates.…”

Section: Discussionmentioning

confidence: 99%

“…HA is a major glycosaminoglycan in uterine fluid; it has been shown to increase cell-cell and cell-matrix adhesion, and may improve embryo apposition and attachment. It has been shown that HA biosynthesis increases dramatically in mice at the time of embryo implantation, and decreases to near-basal levels by the next day [2]. These findings suggest that HA may play a physiological role in implantation.…”

Section: Introductionmentioning

confidence: 95%

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Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

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Purpose To ascertain whether the use of hyaluronanenriched transfer medium (HETM) improves pregnancy and implantation rates among embryo transfer patients with a history of multiple implantation failures. Methods Patients (n0314) under the age of 40 and with a history of multiple unsuccessful embryo transfers were enrolled. There were three groups of patients: those undergoing fresh embryo transfer (fresh ET [n 0111]), those undergoing vitrified-warmed ET in the natural cycle (WET-N [n0101]) and those undergoing WET in a hormone replacement cycle ). On the day of ET, patients were randomized to HETM (0.5 mg/ml hyaluronan) or control medium containing no hyaluronan. Only patients with good quality embryos on day 3 were included. Results For all three patients groups (fresh ET, WET-N and WET-H) pregnancy rates (37.5 %, 31.4 % and 41.2 %, respectively) were significantly higher when using HETM compared with control medium (10.9 %, 10.0 % and 15.7 %, respectively; p<0.05), and implantation rates when using HETM were also significantly higher compared with control medium (p<0.05). Miscarriage rates were similar in both groups. Conclusion HETM significantly increased pregnancy and implantation rates among embryo transfer patients with a history of multiple unsuccessful implantations-regardless of method used to prepare the endometrium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

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“…Several observation could imply that steroids are possibly stimulators of the stromal myxoid changes: increased hyaluronan biosynthesis accompanies decidual responses in the endometrium (26), maternal serum hyaluronic acid concentrations increase as pregnancy progresses and serum levels increase significantly at term (27), hyaluronic acid is associated with cervical ripening during parturition (28), and marked stromal myxoid changes have been reported to be under a strong progestational effect in rectal endometriosis (29). All these reports suggest that the ovarian hormones play an important role in modulating hyaluronic acid synthesis and could provide an explanation of the association of stromal myxoid changes with young age.…”

Section: Discussionmentioning

confidence: 99%

Breast Cancer Stromal Myxoid Changes Are Associated with Tumor Invasion and Metastasis: A Central Role for Hyaluronan

et al. 2003

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The interplay between a tumor and its environment is exemplified by the morphological changes observed in the stroma of human breast cancer. These changes are evident as stromal myxoid changes. Hyaluronan, an extracellular polysaccharide that has been implicated in invasion, is one of the major constituents of the stromal myxoid changes. This study evaluated the association of these stromal changes with axillary node status, tumor grade, and mortality. The prognostic value of the stromal myxoid changes was evaluated in patients with negative axillary nodes with 10 years of follow-up. Our results showed a high level of reproducibility of our stromal myxoid changes grading system (overall kappa ‫؍‬ 0.68). Image analysis semiquantification showed marked correlation of a strong stromal hyaluronan signal with high-grade stromal myxoid changes. In a multiple logistic regression analysis, positive nodes were associated with stromal myxoid changes, tumor size, desmoplasia, lymphocytic infiltration, high tumor grade, tumor emboli, and multifocality. Stromal myxoid changes were also associated with young age and lymphatic embolizations (P < .001). Overall, there is a weak correlation between mortality and stromal myxoid changes (P < .01). Mortality was more evident with high stromal myxoid changes grades and tumor size >2 cm (P < .008). However Cox multivariate analysis fail to show stromal myxoid changes as an independent prognostic factor. In conclusion, stromal myxoid changes with high hyaluronan concentration are strongly associated with positive nodes, tumor grade, and lymphatic emboli, thereby identifying high-risk group and reinforcing the role of hyaluronan in invasion and metastasis.KEY WORDS: Breast cancer, Hyaluronan, Metastasis, Stroma. Mod Pathol 2003;16(2):99 -107Lymph node and hematogenous distant metastases are the result of a cascade of enzymatic interactions. Part of this complex cascade takes place in the tumor environment, where malignant tumor cells detach from the primary tumor, adhere to components of the extracellular matrix, degrade them, and move through the stroma. Morphological and biochemical modifications of the different stromal components (cellular and noncellular) can either favor or inhibit this process. Therefore, the histopathological modifications of the stroma in response to a tumor could shed light on the biological mechanisms involved in invasion and metastasis. Stromal myxoid changes consists of a stromal reaction composed of an amphophilic or slightly basophilic vacuolated material that stains positively with Alcian Blue and is found among the collagen fibers. Hyaluronan is one of its major components. Tissues rich in hyaluronan entrap water and swell, leading to myxoid changes. It is known that hyaluronan synthesis is stimulated by the interaction between tumor and stromal cells (1-3). Hyaluronan has several physiological functions, such as water homeostasis and regulation of capillary growth; it also plays a role in cell recognition and migration (4). A strong relationship betwe...

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“…This may be because (i) of catabolism of collagen by either extracellular proteinases or intracellular digestion; (ii) reduced collagen concentration may reflect an increase in tissue growth through cell division, infiltration of cells, or an increase in the size of cells already present, resulting in a dilution effect on the collagen concentration or (iii) dilution of the extracellular collagen may have been brought about by a non-collagenous matrix component such as hyaluronic acid. A 5-6-fold increase in the concentration of hyaluronic acid has been shown during early pregnancy, although the total amount present is still small relative to collagen (Carson et al, 1987). An important feature of future work will be to assess which, or what, combination of these effects is important.…”

Section: Introductionmentioning

confidence: 97%

Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

Clark¹,

Hurst²,

Myers³

et al. 1992

Reproduction

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Summary. Implantation and non-implantation sites were dissected into myometrial and stromal components; a decidual/embryonic region was obtained on Days 7 and 8 of pregnancy. The concentration of collagen (as a percentage of the dry weight of tissue), measured by hydroxyproline analysis, was significantly lower in the implantation regions than in the non-implant regions in all areas studied. The concentrations in the antimesometrial myometrium and stroma of the implantation region remained the same over the days studied. In contrast, the mesometrial collagen concentration in the implantation region declined from Day 6 to Day 8 of pregnancy. Collagen concentration was low within the decidual/embryonic tissue on Days 7 and 8 of pregnancy. Remodelling of collagen within the embryonic area appears to be an important feature of the uterine response to implantation in rats.

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Glycoconjugate synthesis during early pregnancy: Hyaluronate synthesis and function

Cited by 100 publications

References 15 publications

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Breast Cancer Stromal Myxoid Changes Are Associated with Tumor Invasion and Metastasis: A Central Role for Hyaluronan

Collagen concentrations in dissected tissue compartments of rat uterus on Days 6, 7 and 8 of pregnancy

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