Glycocalyceal bodies in a human rectal carcinoma cell line and their interstitial collagenolytic activities

Murayama, Toshihiko; Kataoka, Hiroaki; Koita, Hiroyuki; Nabeshima, Kazuki; Koono, Masashi

doi:10.1007/bf02899556

Cited by 13 publications

(7 citation statements)

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“…Additionally, immunoelectron microscopy demonstrated shed membrane vesicles which reacted to E l l F 4 in the vicinity of carcinoma cells. Shed membrane vesicles have been reported to be involved in tumor invasion (Nabeshima et al, 1988) and to be associated with collagenolytic activity (Murayama et al, 1991). In vitroconditioned medium from LX-1 cells contains TCSF (data not shown), suggesting the possible presence of a shed-membranelinked form of TCSF.…”

Section: Immunoblot Analysismentioning

confidence: 79%

Enhanced expression of a tumor‐cell‐derived collagenase‐stimulatory factor in urothelial carcinoma: Its usefulness as a tumor marker for bladder cancers

Muraoka

Nabeshima

Murayama

et al. 1993

Intl Journal of Cancer

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A mouse monoclonal antibody (MAb) E11F4, previously raised against the tumor-cell-derived collagenase-stimulatory factor (TCSF) from LX-1 human lung-carcinoma cells, has been used to define the expression and distribution of TCSF in human non-neoplastic urothelium and tumors of the urinary bladder. Immunohistochemically, TCSF was detected in 27/28 transitional-cell carcinomas (TCC) of the bladder, of which 23 were judged to be positive for TCSF according to objective criteria. Twenty-four of 28 non-neoplastic urothelium from 22 individuals were judged to be negative for TCSF by this criteria. However, TCSF immunostaining that was confined to the superficial umbrella cells was frequently observed in non-neoplastic urothelium. In bladder carcinomas, TCSF was in most cases demonstrated in the majority of cells, including at the invasion front. Its localization to the cell membrane was demonstrated by immunoelectron microscopy. The high level of expression of TCSF in bladder tumors, but not in non-neoplastic urothelium, was also demonstrated by immunoblotting of tissue extracts. Furthermore, E11F4 immunostaining identified tumor cells obtained from bladder washings or voided urine and detected more TCC cases than conventional cytology. Since TCSF immunostaining was positive even in low-grade TCC (immunohistochemically and immunocytochemically in 4/5 TCC grade I), the application of TCSF immunostaining to urine cytology appears promising as a valuable adjunct to conventional methods in the clinical evaluation of patients with TCC.

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Section: Immunoblot Analysismentioning

confidence: 79%

Enhanced expression of a tumor‐cell‐derived collagenase‐stimulatory factor in urothelial carcinoma: Its usefulness as a tumor marker for bladder cancers

Muraoka

Nabeshima

Murayama

et al. 1993

Intl Journal of Cancer

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“…For example, highly metastatic variants of the B16 murine melanoma were found to shed more membrane vesicles than did poorly metastatic variants [2], and weakly metastatic cells became more metastatic and colonized similar organs, after fusion with membrane vesicles from the highly metastatic variant [3]. The presence of collagenolytic activity in vesicles shed by human rectal carcinoma cells has also been reported [5]. Vesicles released by murine melanoma cells were reported to contain a metalloproteinase activity (Mr 59 000) [4], and to carry the plasminogen activator (uPA) [1].…”

Section: Introductionmentioning

confidence: 85%

Membrane vesicles shed into the extracellular medium by human breast carcinoma cells carry tumor-associated surface antigens

Dolo¹,

Adobati

Canevari

et al. 1995

Clin Exp Metast

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We have compared the pattern of surface antigen expression, as detected by monoclonal antibodies (mAbs), in plasma membranes vs shed membrane vesicles of two human breast carcinoma cell lines, MCF-7 and 8701-BC. Antigen expression was detected on cells by immunofluorescence (IF) analysis, whilst, due to their small dimensions, the same technique was not applicable to vesicles. For these structures dot-blot analysis and immunoelectron microscopy (IEM) were employed. When applicable, both cell membranes and membrane vesicles were immunoprecipitated and the precipitate (IP) was analyzed by SDS-PAGE. Cells of both lines expressed HLA class I antigens, epithelial cytokeratins, beta 1 integrins, CEA and the glycoprotein detected by mAb 19.9, but only MCF-7 cells expressed Lewis Y, episialin and globo-H antigens and only 8701-BC cells expressed folate receptor. Membrane vesicles of both cell lines appeared to be rich in beta 1, alpha 3 and alpha 5 integrin chains, expressed HLA class I antigens and carried most of the plasma membrane antigens found in the cell membranes. Overall we have analyzed 17 antigens on the two cell lines and on their vesicles. The results obtained for cells (IF and IP) and those for vesicles (dot-blot and IP) were generally concordantly positive or concordantly negative. We obtained a total of 26 clearly concordant combinations on 34 analyses. In three cases we found discordant results, whereas in the remaining combinations we observed slight reactivity and we found difficulties in determining concordance. Discordant results concerned the expression of the following antigens: folate receptors, which were clearly expressed in 8701-BC cells but not detected by dot-blot analysis or IEM on their shed membrane vesicles; neu (c-erb-B2) receptor found in MCF-7 cell membranes but not in their vesicles; and the globo-H antigen recognized by mAb MBr1, detected at low levels on 8701-BC plasma membranes but undetectable on their membrane vesicles. Like vesicles shed in vitro by cultured cells, the vesicles shed in vivo by human breast carcinoma cells could be tagged with several antibodies against tumor-associated antigens. The vesicles shed in vivo were found in association with a fiber network. Some of the fibers had the characteristic fibrin periodicity. These data suggest that tumor markers detected in the circulation of carcinoma patients, at least in part, are carried by shed membrane vesicles. Moreover the observation that membrane vesicles carry both tumor-associated antigens and HLA class I molecules indicate that these structures could in principle present antigens to the immune system.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Although they resemble the viruses which have been reported in the sublingual gland (Sato and Miyoshi, 1983), the granules are smaller than the viruses and lack capsomere. In appearance, these granules correspond to the glycocalyceal bodies found on the apical surface of normal and neoplastic intestinal-type epithelial cells (Shnitka and Alta, 1964;Marcus et al, 1979;Ghadially, 1985;Murayama et al, 1991;Ohiwa et al, 1994), respiratory tract (Afzelius, 1984;Ellinger et al, 1987) and esophagus (Ozzello et al, 1977). Their function and nature still are unknown.…”

Section: Discussionmentioning

confidence: 99%

Fine structure of tuft cells of the main excretory duct epithelium in the rat submandibular gland

Sato¹,

Miyoshi²

1997

Anat. Rec.

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Background Tuft cells, which are characterized by long microvilli with prominent rootlets and by vesicular and tubular profiles, are present in the mucosal epithelium of a number of hollow organs, including the main excretory duct of the rat submandibular gland. Despite their widespread occurrence, little is known of their function. Methods Main excretory ducts of the submandibular gland were obtained from 20 male Wistar rats and prepared for electron microscopic examination. Specimens also were subjected to various histochemical procedures, including HRP (horseradish peroxidase) uptake, the demonstration of catalase in peroxisomes, glycoconjugate cytochemistry, and ruthenium red staining. Results The features of the tuft cells in the MED (main excretory duct) basically were similar to those described in other organs. However, for the first time it was observed that in the submicrovillus zone and intermicrovillus space, there were many membrane‐bound electron‐dense granules, which resemble the so‐called glycocalyceal bodies found on the apical surface of normal and neoplastic intestinal‐type epithelial cells. Hypolemmal nerve terminals were seen to be in contact with the basal portion of tuft cells. After injection of HRP, reaction products were observed on the luminal surface of the microvilli, but not within the vesicles. Vesicles and tubules in the supranuclear cytoplasm exhibited a positive cytochemical reaction for glycoconjugates. The surface coat of tuft cell microvilli was stained with ruthenium red, but the vesicles and tubules in the supranuclear cytoplasm remained unstained. Conclusions Our observations suggest that the tuft cells may engage in secretory activity in addition to reception. Anat. Rec. 248:325‐331, 1997. © 1997 Wiley‐Liss, Inc.

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Glycocalyceal bodies in a human rectal carcinoma cell line and their interstitial collagenolytic activities

Cited by 13 publications

References 13 publications

Enhanced expression of a tumor‐cell‐derived collagenase‐stimulatory factor in urothelial carcinoma: Its usefulness as a tumor marker for bladder cancers

Enhanced expression of a tumor‐cell‐derived collagenase‐stimulatory factor in urothelial carcinoma: Its usefulness as a tumor marker for bladder cancers

Membrane vesicles shed into the extracellular medium by human breast carcinoma cells carry tumor-associated surface antigens

Fine structure of tuft cells of the main excretory duct epithelium in the rat submandibular gland

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