Glyco-gold nanoparticles: synthesis and applications

Compostella, Federica; Pitirollo, Olimpia; Silvestri, Alessandro; Polito, Laura

doi:10.3762/bjoc.13.100

Cited by 78 publications

(62 citation statements)

References 107 publications

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“…Spherical citrate-stabilized GNPs with 15 nm average diameter (which are often considered as standard GNPs for immunochemical studies due to ease of preparation and conjugation with ligands [48,56,57] and have successfully been used in our previous studies [56,57,77,78,95]) were chosen for the experiments. This is because GNPs of this size, unlike much smaller GNPs (e.g., around 2 nm in diameter), which are often derivatized in situ [32,39,46,48], can be easily prepared in advance and kept for further conjugation with a desired ligand. Another advantage of using these GNPs is their strong light absorption due to localized surface plasmon resonance (LSPR) around 520 nm while colloids of GNPs smaller than 3 nm do not exhibit an LSPR and are barely colored.…”

Section: Glyco-gnpsmentioning

confidence: 99%

“…It is known that the color of GNP colloids dramatically depends on size and shape of the GNPs as well as on the dielectric properties of the medium surrounding the GNPs. These features are useful in many (bio)analytical applications including monitoring their stability (as described below) [25,26,46]. Size and shape of the GNPs (spheres, nanorods, nanoshells and nanostars) were shown to influence the immunogenicity of conjugates with haptens.…”

Section: Glyco-gnpsmentioning

confidence: 99%

“…For the preparation of glyco-GNPs, glycans are usually transformed to glycosides with thiol-functionalized spacer aglycons that are conjugated [43,44] either with pre-formed citrate-stabilized GNPs [67] or during the in situ synthesis of glyco-GNPs [32,38,46]. It is also possible to conjugate suitably derivatized Figure 1: Structures of the hexasaccharide fragments of LAM/AG with amino groups at the terminal position of short (С 2 ) or elongated (С 2 (ЕG) 7 ) spacer aglycons (Ara 6 glycosides 1 and 2, respectively) and their conjugates with gold nanoparticles (GNPs) (Ara 6 -GNPs 3 and 4, respectively).…”

Section: Introductionmentioning

confidence: 99%

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Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2020

Beilstein J. Nanotechnol.

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A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while there was only a weak response to M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.

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Section: Glyco-gnpsmentioning

confidence: 99%

Section: Glyco-gnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2020

Beilstein J. Nanotechnol.

View full text Add to dashboard Cite

show abstract

“…Gold nanoparticles (AuNPs) [13,14] in conjugation with glycans [13][14][15][16][17][18] enable them with high aqueous solubility/dispersibility, biocompatibility and their high surface area/volume ratio of AuNPs allows enhanced sensitivity. Carbohydrate-lectin analyses have been enabled by the application of AuNPs.…”

Section: Glycans and Nanotechnologymentioning

confidence: 99%

Nanotechnology and sialic acid biology

Ghosh¹

2020

Sialic Acids and Sialoglycoconjugates in the Biology of Life, Health and Disease

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“…The combination of GNPs based sample preparation with MALDI-TOF-MS analysis has become quite popular recently as it showed significant improvements of the shotto-shot reproducibility and ionization efficiency; it furthermore improved the sample homogeneity, reduced the sample preparation time, and eliminated matrix ion interferences as observed with common MALDI matrices in the low m/z range [55,[160][161][162][179][180][181][182][183][184][185][186].…”

Section: Application Of Gnps For Biomarker Analysismentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

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Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

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Glyco-gold nanoparticles: synthesis and applications

Cited by 78 publications

References 107 publications

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Nanotechnology and sialic acid biology

Functionalized gold nanoparticles for sample preparation: A review

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