Glycine feeding improves pristinamycin production during fermentation including resin for in situ separation

Abstract

Seven amino acids were tested as precursors to affect pristinamycin production by a mutant strain derived from Streptomyces pristinaespiralis ATCC25486. Of those, glycine was selected as the best precursor to facilitate both cell growth and pristinamycin production at the feeding time of 36-h incubation and the feeding rate of 0.75 g L -1 flask culture. The optimized time and concentration of glycine feeding were applied to enlarged 3-L bioreactor fermentation with a resin added at the time of 20-h fermentation for in situ separation. As a result, a combination of the glycine feeding and the added resin resulted in the maximal pristinamycin yield of 616 mg L -1 culture 12 h after glycine feeding. The yield from the combined treatment was 1.71-, 2.77-and 4.32-fold of those from the mere glycine and resin treatments and the control, respectively. Other parameters, including intracellular nucleic acid content, animo nitrogen content and pH level, during 72-h fermentation were also given in association with the pristinamycin yields in the different treatments. The results indicate that glycine feeding is an effective approach to enhance pristinamycin production in the culture of S. pristinaespiralis F213 with supplemented resin for in situ separation.

Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

“…A correct adsorbent selection can provide a very significant (sometimes 100-fold) yield increase (Singh et al 2010). In spite of a large number of publications describing the use of adsorbing resins in the microbial production of biologically active substances, we did not find any information on the use of such resins in the virginiamycin production, though the use of adsorbing resins was reported to increase the production of a similar antibiotic, pristinamycin, by 1.25–1.55 times (Jia et al 2006, Zhang et al 2012). …”

“…In spite of a wide use of this approach in the microbial production of biologically active substances including pristinamycin, an antibiotic, which is very similar to virginiamycin (Jia et al 2006; Zhang et al 2012), authors did not find any data for virginiamycin. The only known patent, mentioning the use of an unknown Dow macroporous resin in the virginiamycin biosynthesis, described the application of this resin as a tool to improve gas exchange in the fermentation medium (Yong 2015).…”

Diversity and prevalence of ANTAR RNAs across actinobacteria

“…Another ANTAR-target RNA is found in the mRNA for the enzyme agmatinase ( SPRI_RS23705 ), that converts arginine to putrescine. Putrescine is a precursor of succinate [ 63 , 64 ] that can feed into the TCA cycle and the synthesis of various amino acids, which are directly involved in the production of the antibiotic pristinamycin [ 65 , 66 ]. The discovery of these ANTAR-target RNAs in Streptomyces thus implicates gene SPRI_RS32325 and SPRI_RS23705 as possible candidates that might be investigated to understand the observed phenotype.…”

Diversity and prevalence of ANTAR RNAs across actinobacteria

“…Putrescine is a precursor of succinate (Krysenko et al 2017;Schneider and Reitzer 2012) that can feed into the TCA cycle and the synthesis of various amino acids, which are directly involved in the production of the antibiotic pristinamycin (Voelker and Altaba 2001;Zhang et al 2012). The discovery of these ANTAR-target RNAs in Streptomyces thus implicates gene SPRI_RS32325 and SPRI_RS23705 as possible candidates that might be investigated to understand the observed phenotype.…”