Glycine adversely affects enhanced biological phosphorus removal

Tian, Yucheng; Chen, Hang; Chen, Liping; Deng, Xuhan; Hu, Zekun; Wang, Cenchao; Wei, Chaohai; Qiu, Guofu; Wuertz, Stefan

doi:10.1016/j.watres.2021.117894

Cited by 23 publications

(11 citation statements)

References 60 publications

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“…49 Recently, it was shown that glycine can induce P release from PAOs. 15,50,51 Accumulibacter 15 and Tetrasphaera spp. 50 were able to consume glycine with and without PHA synthesis, respectively.…”

Section: Effect Of Carbon Source On P Re-dissolutionmentioning

confidence: 99%

Phosphorus recovery by re-dissolution from activated sludge – effects of carbon source and supplementation level revisited

Anders¹,

Weigand²,

Platen³

2023

Environ. Sci.: Water Res. Technol.

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Section: Effect Of Carbon Source On P Re-dissolutionmentioning

confidence: 99%

Phosphorus recovery by re-dissolution from activated sludge – effects of carbon source and supplementation level revisited

Anders¹,

Weigand²,

Platen³

2023

Environ. Sci.: Water Res. Technol.

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“…A maximum likelihood tree was built based on the concatenation of single-copy genes with model Q. insect+F+I+I+R4. Genomes in red are those recovered from our bioreactors (Arumugam et al, 2019; Qiu et al, 2020; Tian et al, 2022; Deng et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

“…The genomes in red were recovered from our lab-scale reactors. SSA1, SSB1 and SCUT-1 were recovered in our previous work (Arumugam et al, 2019;Tian et al, 2022). SCELSE-5, SCELSE-7, SCELSE-10, and SCUT-2 were recovered from three of our lab-scale EBPR reactors (Supplementary Materials).…”

Section: Phylogenetic Analysis Of Pangenomementioning

confidence: 96%

“…With the advance in EBPR research and sequencing techniques, increasing numbers of high-quality Ca . Accumulibacter genomes have been obtained (Arumugam et al, 2019; Rubio-Rincón et al; 2019; Qiu et al, 2020; McDaniel et al, 2021; Singleton et al, 2021; Srinivasan et al, 2021; Tian et al, 2022; Petriglieri et al, 2022; Zhang et al, 2023; Deng et al, 2023). Additionally, new PAOs and glycogen accumulating organisms (GAOs) were identified in genera phylogenetically closely related to Ca .…”

Section: Introductionmentioning

confidence: 99%

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Integrated genomics provides insights for the evolution of the polyphosphate accumulation trait ofCa.Accumulibacter

Xie,

Deng,

Chen

et al. 2023

Preprint

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Candidatus Accumulibacter plays a major role in enhanced biological phosphorus removal (EBPR), but the key genomic elements in metagenome assembled genomes enabling their phosphorus cycling ability remain unclear. Pangenome analyses were performed to systematically compare the genomic makeup of Ca. Accumulibacter and non-Ca. Accumulibacter members within the Rhodocyclaceae family. Metatranscriptomic analyses of an enrichment culture of Ca. Accumulibacter clade IIC strain SCUT-2 were performed to investigate gene transcription characteristics in a typical anaerobic-aerobic cycle. Two hundred ninety-eight core genes were shown to be obtained by Ca. Accumulibacter at their least common ancestor. One hundred twenty-four of them were acquired via horizontal gene transfer (HGT) based on best-match analysis against the NCBI database. Fourty-four laterally derived genes were actively transcribed in a typical EBPR cycle, including the polyphosphate kinase 2 (PPK2) gene. Genes in the phosphate regulon (Pho) were poorly transcribed. Via a systematical analysis of the occurrences of these genes in closely related Dechloromonas-polyphosphate accumulating organisms (PAOs) and Propionivibrio-non-PAOs, a Pho dysregulation hypothesis is proposed to explain the mechanism of EBPR. It states that the PhoU acquired by HGT fails in regulating the high-affinity phosphate transport (Pst) system. To avoid phosphate poisoning, the laterally acquired PPK2 is employed to condense excess phosphate into polyphosphate. Alternatively, genes encoding PhoU and PPK2 are obtained from different donor bacteria, leading to unmatched phosphate concentration thresholds for their activation/inactivation. PPK2 tends to reduce the intracellular phosphate to concentration levels perceived by PhoU as low-phosphate states. PhoU is not activated to turn off the Pst system, resulting in continuous phosphate uptake. In conclusion, based on integrated genomic analyses, the HGT of phoU and ppk2 and the resultant Pho dysregulation may have triggered the development and evolution of the P cycling trait in Ca. Accumulibacter.

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“…Fourty-four Ca . Accumulibacter genomes were recovered from three lab-scale SBRs (NTU35 and NTU30 (Qiu et al, 2022), and SCUT (Tian et al, 2022)). Ten high-quality (completeness >95%, contamination <6%) Ca .…”

Section: Resultsmentioning

confidence: 99%

CRISPR-Cas phage defense systems and prophages inCandidatusAccumulibacter

Deng

Yuan

Chen

et al. 2022

Preprint

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Candidatus Accumulibacter is a key genus of polyphosphate-accumulating organisms (PAOs) found in laboratory- and full-scale wastewater treatment systems, mediating enhanced biological phosphorus removal (EBPR). However, little is known about their ability to resist phage infection. We conducted a systematic analysis of the occurrence and characteristics of clustered regularly interspaced short palindromic repeats and associated proteins (CRISPR-Cas) systems and prophages in diverse Ca. Accumulibacter taxa (43 in total, including 10 newly recovered genomes). Fourty complete CRISPR loci were identified in 28 genomes, falling into 6 subtypes. The occurrence and distribution of CRISPR-Cas systems did not follow a vertical evolutionary relationship. Phylogenetic analyses of the cas genes and direct repeats (DRs) suggested that the CRISPR-Cas systems were likely acquired via horizontal gene transfer, with acquisition rates higher than those of speciation, rendering different Ca. Accumulibacter distinct adaptivity to phage predations. 2448 spacers were identified, 67 of them matched to known phages. Major differences were observed among the numbers of spacers for different Ca. Accumulibacter, showing unique phages that could be resisted by different members. A comparison of the spacers in genomes having the same cas gene but from distinct geographical locations indicated that habitat isolation may have resulted in the acquisition of different spacers by different Ca. Accumulibacter. Metagenomic analysis allowed the identification of 26 viral contigs (18 are Caudovirales members) in 6 metagenomic datasets from three lab-scale enrichment reactors, matching to 73 spacers in 10 Ca. Accumulibacter genomes in these reactors, showing the specific immunity of these Ca. Accumulibacter. Metatranscriptomic analyses showed the activity of the CRISPR-Cas system under both anaerobic and aerobic conditions. Extra efforts were made to identify prophages in Ca. Accumulibacter genomes. In total, 133 prophage regions were identified. Twenty-seven of them were classified as potentially active prophages. Three prophages (all are Caudovirales members, in DS2011, SCELSE-7IIH and SCELSE-5IIH, respectively) are readily activable. Differences in the occurrence of CRISPR-Cas systems and prophages in Ca. Accumulibacter will lead to their distinct responses under phage predation. This study represents the first systematic analysis of CRISPR-Cas systems and prophages with combined experimental and bioinformatic methods in the Ca. Accumulibacter lineage, providing new perspectives on the potential impacts of phages on Ca. Accumulibacter and EBPR systems.

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Glycine adversely affects enhanced biological phosphorus removal

Cited by 23 publications

References 60 publications

Phosphorus recovery by re-dissolution from activated sludge – effects of carbon source and supplementation level revisited

Phosphorus recovery by re-dissolution from activated sludge – effects of carbon source and supplementation level revisited

Integrated genomics provides insights for the evolution of the polyphosphate accumulation trait ofCa.Accumulibacter

CRISPR-Cas phage defense systems and prophages inCandidatusAccumulibacter

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