Glycidyl methacrylate-co-N-vinyl-2-pyrrolidone coated polypropylene strips: Synthesis, characterization and standardization for dot-enzyme linked immunosorbent assay

Polyacrylonitrile Fiber as Matrix for Immunodiagnostics

“…Thus, covalent binding on modified PAN fibers leads to high sensitivity and specificity. The advantages of covalent binding are also reported by other authors [Palma, et al, 2004;Tedeschi, et al 2003;Tyagi, et al, 2009]. From these studies it can be concluded that modified PAN fibers as a solid support are more sensitive, specific and cost effective as compared to PS 96-well microtiter plates.…”

Balancing the decomposable behavior and wet tensile mechanical property of cellulose-based wet wipe substrates by the aqueous adhesive

“…Finally, in view of the synthetic NVP-GMA adhesive, there are already researchers reposted the hydrophilic porous magnetic poly (GMA-MBAA-NVP) composite microspheres containing oxirane groups as an efficient carrier for immobilizing penicillin G acylase [ 53 ]. Tyagi et al synthesized GMA-co-NVP copolymers with various GMA:NVP ratios and dip coated it onto polypropylene (PP) strips [ 49 ]. All the polymer coated PP strips were evaluated for their application in dot-ELISA (enzyme-linked immunosorbent assay) using a model system, showing that the best results were obtained with GMA-NVP copolymer of 78:22 mol% ratio of GMA and NVP respectively, coated onto the PP strips.…”

“…Most importantly, the NVP monomer can copolymerize with GMA easily [ 48 ]. More importantly, the previous researchers thoroughly investigated the FT-IR, 1 H NMR spectra and structural characterizations about the NVP-GMA copolymers [ [49] , [50] , [51] ], which laid the scientific foundation for our follow-up work.…”

“…In this work, we described a novel N-vinyl pyrrolidone-glycidyl methacrylate (NVP-GMA) adhesive which contributes the mechanical strength enhancement of the cellulose-based material in the wet state. Meanwhile, the chemical synthesis of NVP-GMA copolymers by solution polymerization method using AIBN (same as this work) as free radical initiator has been extensively studied before [ 49 , 52 , 53 ]. The NVP-GMA adhesive was introduced into the cellulose fibers to prepare the decomposable cellulose-based wet wipe substrate with suitable wet tensile index by a wet forming method.…”

Development and validation of dot-ELISA on modified cellulose filter paper: a simplified novel approach

“…[33][34][35][36] Dot-ELISAa highly versatile solid phase immunoassay for antibody or antigen detectionhas been widely employed in several serological tests because of its sensitivity and specicity in addition to being a practical and less expensive diagnostic test, not requiring either sophisticated equipment or trained technical personnel. 37 The present paper aims at the evaluation and standardization of GMA graed CFP (with GMA gra% from 10 to 100), developed and reported previously by the same group of investigators, in dot-ELISA. 38 The paper also describes the practical application of CFP-g-GMA surfaces for identication of human blood and the source of mosquito blood meal (species on which mosquito fed).…”