Glycerylphosphorylcholine in the Male Reproductive Organs of Rats and Guinea‐pigs

Dawson, R. M. C.; Rowlands, I. W.

doi:10.1113/expphysiol.1959.sp001373

Cited by 83 publications

(39 citation statements)

References 3 publications

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“…Dawson and Rowlands (1959) found that when [32P]orthophosphate was injected into rats it was incorporated into the GPC of the epididymis within several hours and we have confirmed and extended this observation using the rabbit (Scott, Wales, et al 1963). The synthesis occurs when the epididymis is separated from the testes and will take place even when slices of rabbit epididymis are incubated with 32p in vitro.…”

Section: Introduotionsupporting

confidence: 72%

“…Phospholipid has been demonstrated in vacuoles of the epithelial cells of the mouse epididymis (Christie 1955) and this has been suggested as a possible precursor of GPC (Dawson, Mann, and White 1957;Dawson and Rowlands 1959). Further experiments to elucidate the mode of synthesis of GPC in vitro by the caput epididymis of the rabbit are described in this paper.…”

Section: Introduotionmentioning

confidence: 87%

“…The synthesis occurs when the epididymis is separated from the testes and will take place even when slices of rabbit epididymis are incubated with 32p in vitro. Both the head and the tail regions of the epididymis can synthesize GPC, but the head is more active (Dawson and Rowlands 1959;Scott, Wales, et al 1963;Scott, Dawson, and Rowlands 1963).…”

Section: Introduotionmentioning

confidence: 99%

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The Respiration of the Rabbit Epididymis and Its Synthesis of Glycerylphosphorylcholine

Jc¹,

Wales²,

White³

1966

Aust. Jnl. Of Bio. Sci.

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SummaryThe synthesis of glycerylphosphorylcholine (GPO) has been studied in the caput epididymis of the rabbit in vitro using [32P]orthophosphate and choline. The incorporation of 32p or 14C into GPO was much greater than into lecithin and the ratio of 32p to 140 was much less in the GPC than in lecithin. Coenzyme A and stearate caused a ~ixty-fold stimulation of the incorporation of 32p into lecithin but only a sevenfold stimulation of incorporation into GPO. The results of these experiments are not consistent with lecithin being the major immediate precursor of GPC, in vitro.The Qo, values for caput epididymis tissue varied from a maximum of 2·2 for tissue slices to 0·5 for some homogenates. Oxygen uptake of tissue slices in Ringerglucose was similar to that in a complex medium containing most cofactors. The addition of 10 fLg/ml testosterone to the incubation mixture did not affect the oxygen uptake of either tissue slices or homogenates. However, tissue slices of mature epididymides' respired at a greater rate than those obtained from immature animals.

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Section: Introduotionsupporting

confidence: 72%

Section: Introduotionmentioning

confidence: 87%

Section: Introduotionmentioning

confidence: 99%

See 1 more Smart Citation

The Respiration of the Rabbit Epididymis and Its Synthesis of Glycerylphosphorylcholine

Jc¹,

Wales²,

White³

1966

Aust. Jnl. Of Bio. Sci.

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“…Epididymal secretion Not much new information on epididymal secretion has been published since the pioneer studies by Dawson & Rowlands (1959) on the secretion of glycerylphosphorylcholine and by Crabo (1965) on the ionic and organic aspects of epididymal absorption and secretion. One notable exception to this generaliz¬ ation is the micropuncture study by Levine & Marsh (1971) in anaesthetized rats.…”

Section: Mechanism Of Secretion and Its Controlmentioning

confidence: 99%

Secretions of the Testis and Epididymis

Bp¹

1974

Reproduction

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“…It is generally believed that epididymal tissue is responsible for the production of most of the GPC found in semen (Dawson, Mann, and White 1957;Dawson and Rowlands 1959;Wallace, Wales, and White 1966). The loss of fatty acids and aldehydes from spermatozoal phospholipids during their passage through the epididymis could, however, account for about 20% of the GPC in the cauda epididymis of the ram.…”

Section: (D) Permeability Of Spermaiozoa After Cold Shockmentioning

confidence: 99%

Phospholipid and Cholesterol Content of Epididymal and Jaculated Ram Spermatozoa and Seminal Plasma in Elation to Cold Shock

Quinn¹,

White²

1967

Aust. Jnl. Of Bio. Sci.

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SummaryThe concentration of total phospholipid, phosphatidylcholine, phosphatidylethanolamine, and choline plasmalogen in spermatozoa from the caput epididymis of the ram was higher than in spermatozoa from the cauda. The phospholipid composition of spermatozoa from the latter region of the epididymis more closely resembled ejaculated spermatozoa.In general, the plasma of caput and cauda semen was similar in total phospholipid content and composition.The total phospholipid concentration of ejaculated seminal plasma was only about one-fifth that of the caput and cauda fluid and all phospholipid fractions were greatly reduced.The glycerylphosphorylcholine content of caput spermatozoa and seminal plasma was less than that of the corresponding semen fractions obtained from the cauda epididymis, and after ejaculation.The concentration of non-esterified cholesterol in both the spermatozoa and plasma decreased on passing from the caput to the cauda epididymis and was even less in the corresponding fractions of ejaculated semen.Spermatozoa taken from the testis and caput epididymis were little affected by cold shock whereas spermatozoa from the cauda epididymis and ampulla were much more susceptible, as judged by increased permeability to stain. It is suggested that a decrease in the phospholipid and cholesterol content of spermatozoa may be associated with an increase in the susceptibility of ram spermatozoa to cold shock as they pass through the male reproductive tract.

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Glycerylphosphorylcholine in the Male Reproductive Organs of Rats and Guinea‐pigs

Cited by 83 publications

References 3 publications

The Respiration of the Rabbit Epididymis and Its Synthesis of Glycerylphosphorylcholine

The Respiration of the Rabbit Epididymis and Its Synthesis of Glycerylphosphorylcholine

Secretions of the Testis and Epididymis

Phospholipid and Cholesterol Content of Epididymal and Jaculated Ram Spermatozoa and Seminal Plasma in Elation to Cold Shock

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