Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34melT

Paván, María Elisa; Venero, Esmeralda C. Solar; Egoburo, Diego E.; Pavan, E.; López, Nancy I.; Pettinari, M. Julia

doi:10.1007/s00253-018-9545-9

Cited by 10 publications

(9 citation statements)

References 52 publications

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“…In A. salmonicida subsp. pectinolytica, the presence of glycerol was observed to abolish melanin synthesis through a combination of metabolic and regulatory effects, including the generation of a more reduced environment, unfavorable for homogentisate oxidation, and a significant inhibition of hpd transcription (Pavan et al 2019).…”

Section: Control Of Melanin Synthesis In Bacteriamentioning

confidence: 99%

Melanin biosynthesis in bacteria, regulation and production perspectives

Paván

López

Pettinari

2019

Appl Microbiol Biotechnol

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The production of black pigments in bacteria was discovered more than a century ago and related to tyrosine metabolism. However, their diverse biological roles and the control of melanin synthesis in different bacteria have only recently been investigated. The broad distribution of these pigments suggests that they have an important role in a variety of organisms. Melanins protect microorganisms from many environmental stress conditions, ranging from ultraviolet radiation and toxic heavy metals to oxidative stress. Melanins can also affect bacterial interactions with other organisms and are important in pathogenesis and survival in many environments. Bacteria produce several types of melanin through dedicated pathways or as a result of enzymatic imbalances in altered metabolic routes. The control of the melanin synthesis in bacteria involves metabolic and transcriptional regulation, but many aspects remain still largely unknown. The diverse properties of melanins have spurred a large number of applications, and recent efforts have been done to produce the pigment at biotechnologically relevant scales.

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Section: Control Of Melanin Synthesis In Bacteriamentioning

confidence: 99%

Melanin biosynthesis in bacteria, regulation and production perspectives

Paván

López

Pettinari

2019

Appl Microbiol Biotechnol

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“…Guanine crystals are associated with melanin in many organisms 2,32,33 . In bacteria that produce homogentisate melanin such as 34mel, melanin synthesis can be inhibited by the herbicide bicyclopyrone 34 . When 34mel was grown in the presence of this inhibitor crystals with similar morphology were observed, although delayed by several days ( SI Appendix , Fig.…”

Section: Resultsmentioning

confidence: 99%

“…After 5 days incubation cultures were kept at room temperature or 4ºC and crystals formation was followed using an Olympus Tokyo CK inverted microscope or a stereoscopic microscope Nikon SMZ-745T. For melanin synthesis inhibition 1 mM bicyclopyrone was added to the growth medium 34 .…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Guanine crystals discovered in bacteria

Paván

Movilla

Pavan

et al. 2022

Preprint

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Guanine crystals are the most common organic biogenic crystals, found in many organisms1. Due to their exceptionally high refractive index, they contribute to structural color and are responsible for the reflective effect in the skin and visual organs in many animals, such as fish, reptiles and spiders2. Occurrence of these crystals in animals has been known for many years, and they have also been observed in eukaryotic microorganisms3, but not in prokaryotes. In this work we report the discovery of extracellular crystals in a melanogenic Aeromonas, and reveal that they are composed of guanine, and particularly the unusual monohydrate form. We also reveal the occurrence of these crystals in other bacteria, characterize them and investigate the metabolic traits related to their synthesis. In all cases studied the presence of the guanine crystals in bacteria correlated with the absence of guanine deaminase, which could lead to guanine accumulation providing the substrate for crystal formation. Our finding of the hitherto unknown guanine crystal occurrence in prokaryotes extends the range of guanine crystal producing organisms to a new Domain of life. Bacteria constitute a new and more accessible model to study the process of guanine crystal formation and assembly. This discovery opens countless chemical and biological questions, including those about the functional and adaptive significance of their production in these microorganisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications.

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“…HPPD protein sequences of the three bacteria previously studied were aligned (Supplementary Information 3), together with the HPPD of R. etli CFN42, which was available online and was part of our first experiment, and five others HPPD sequences from different bacteria strains, whose pyomelanin production has been described [13,19,63,64]. Even though these nine sequences have relatively low similarity, ranging from 35.26 to 87.39%, they retain some highly conserved regions.…”

Section: In Silico Hppd Sequences Analysismentioning

confidence: 99%

Assessing the effects of β-triketone herbicides on HPPD from environmental bacteria using a combination of in silico and microbiological approaches

Thiour-Mauprivez

Dayan

Terol

et al. 2022

Environ Sci Pollut Res

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4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of β-triketone herbicides in plants. This enzyme, involved in the tyrosine pathway, is also present in a wide range of living organisms, including microorganisms. Previous studies, focusing on a few strains and using high herbicide concentrations, showed that β-triketones are able to inhibit microbial HPPD. Here, we measured the effect of agronomical doses of β-triketone herbicides on soil bacterial strains. The HPPD activity of six bacterial strains was tested with 1 or 10 the recommended field dose of the herbicide sulcotrione. The selected strains were tested with 0.01 to 15 the recommended field dose of sulcotrione, mesotrione and tembotrione. Molecular docking was also used to measure and model the binding mode of the three herbicides with the different bacterial HPPD. Our results show that responses to herbicides are strain-dependent with Pseudomonas fluorescens F113 HPPD activity not inhibited by any of the herbicide tested; when all three β-triketone herbicides inhibited HPPD in Bacillus cereus ATCC14579 and Shewanella oneidensis MR-1. These responses are also molecule-dependent with tembotrione harboring the strongest inhibitory effect. Molecular docking also reveals different binding potentials. This is the first time that the inhibitory effect of β-triketone herbicides is tested on environmental strains at agronomical doses, showing a potential effect of these molecules on the HPPD enzymatic activity of non-target microorganisms.

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Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34melT

Cited by 10 publications

References 52 publications

Melanin biosynthesis in bacteria, regulation and production perspectives

Melanin biosynthesis in bacteria, regulation and production perspectives

Guanine crystals discovered in bacteria

Assessing the effects of β-triketone herbicides on HPPD from environmental bacteria using a combination of in silico and microbiological approaches

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