Abstract:The success of xanthan biosynthesis depends on several factors, most importantly the genetic potential of the production microorganism and cultivation media composition. Cultivation media composition affects the yield and quality of the desired product as well as production costs. This is why many studies focus on finding cheap alternative raw materials, especially carbon sources, to replace commercially used glucose and sucrose. In addition to the Xanthomonas campestris ATCC 13951 which is the primary industr… Show more
“…The quality of xanthan can be estimated based on several parameters, such as the viscosity of its solutions, the composition of the macromolecules, the molecular weight, etc. [18]. In this study, the quality of the xanthan produced in applied experimental conditions was estimated based on its average molecular weight.…”
Section: Discussionmentioning
confidence: 99%
“…Besides the producing strain, cultivation time also has significant effect on xanthan quantity and quality [17]. Generally, fermentation time for xanthan production on glycerol containing media ranges from 48 h to 168 h [14,18,19]. The results from earlier studies show that after the cultivation of certain Xanthomonas strains on glycerol-based media for 96 h [14] and 120 h [18], glycerol conversion was about 50% or less.…”
Section: Introductionmentioning
confidence: 99%
“…Generally, fermentation time for xanthan production on glycerol containing media ranges from 48 h to 168 h [14,18,19]. The results from earlier studies show that after the cultivation of certain Xanthomonas strains on glycerol-based media for 96 h [14] and 120 h [18], glycerol conversion was about 50% or less. The applied producing strains did not metabolize all available amount of glycerol probably due to lack of time to adapt to glycerol and produce xanthan in a sufficient quantity.…”
In this study, the effect of cultivation time on xanthan biosynthesis by different Xanthomonas campestris and Xanthomonas euvesicatoria strains, isolated from crucifers and pepper leaves, respectively, was examined. Xanthan was produced by submerged cultivation on crude glycerol-based medium at a laboratory level under aerobic conditions at 30 °C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan concentration in media at the end of bioprocess and its average molecular weight. According to the obtained results, Xanthomonas strains have statistically significant effect on xanthan concentration in cultivation media when biosynthesis is performed by X. euvesicatoria strains, and cultivation time has significant effect on this parameter only when bioprocess is performed by X.campestris strains. The combination of Xanthomonas strains and cultivation time has a statistically significant effect on xanthan concentration in medium for both groups of isolates. The obtained results show that all applied Xanthomonas strains and cultivation time as well as their combination have statistically significant effect on average molecular weight of xanthan produced in applied experimental conditions. It is found that X. euvesicatoria strains produce higher amount of xanthan in a shorter period of time (168 h) when compared to the X.campestris strains. Xanthan of higher average molecular weight was produced when cultivation of both groups of isolates was performed for 240 h in applied experimental conditions. Results obtained in this research suggest that X. euvesicatoria strains have the greatest potential for application in biotechnological production of xanthan on crude glycerol-based medium.
“…The quality of xanthan can be estimated based on several parameters, such as the viscosity of its solutions, the composition of the macromolecules, the molecular weight, etc. [18]. In this study, the quality of the xanthan produced in applied experimental conditions was estimated based on its average molecular weight.…”
Section: Discussionmentioning
confidence: 99%
“…Besides the producing strain, cultivation time also has significant effect on xanthan quantity and quality [17]. Generally, fermentation time for xanthan production on glycerol containing media ranges from 48 h to 168 h [14,18,19]. The results from earlier studies show that after the cultivation of certain Xanthomonas strains on glycerol-based media for 96 h [14] and 120 h [18], glycerol conversion was about 50% or less.…”
Section: Introductionmentioning
confidence: 99%
“…Generally, fermentation time for xanthan production on glycerol containing media ranges from 48 h to 168 h [14,18,19]. The results from earlier studies show that after the cultivation of certain Xanthomonas strains on glycerol-based media for 96 h [14] and 120 h [18], glycerol conversion was about 50% or less. The applied producing strains did not metabolize all available amount of glycerol probably due to lack of time to adapt to glycerol and produce xanthan in a sufficient quantity.…”
In this study, the effect of cultivation time on xanthan biosynthesis by different Xanthomonas campestris and Xanthomonas euvesicatoria strains, isolated from crucifers and pepper leaves, respectively, was examined. Xanthan was produced by submerged cultivation on crude glycerol-based medium at a laboratory level under aerobic conditions at 30 °C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan concentration in media at the end of bioprocess and its average molecular weight. According to the obtained results, Xanthomonas strains have statistically significant effect on xanthan concentration in cultivation media when biosynthesis is performed by X. euvesicatoria strains, and cultivation time has significant effect on this parameter only when bioprocess is performed by X.campestris strains. The combination of Xanthomonas strains and cultivation time has a statistically significant effect on xanthan concentration in medium for both groups of isolates. The obtained results show that all applied Xanthomonas strains and cultivation time as well as their combination have statistically significant effect on average molecular weight of xanthan produced in applied experimental conditions. It is found that X. euvesicatoria strains produce higher amount of xanthan in a shorter period of time (168 h) when compared to the X.campestris strains. Xanthan of higher average molecular weight was produced when cultivation of both groups of isolates was performed for 240 h in applied experimental conditions. Results obtained in this research suggest that X. euvesicatoria strains have the greatest potential for application in biotechnological production of xanthan on crude glycerol-based medium.
“…So, there is also a need to determine the optimal cultivation time for new producing strains (18) in order to achieve successful efficacy of examined biotechnological process. The usual fermentation time for xanthan production is from 72 h to 120 h (15,19,20), and in one study, xanthan biosynthesis was performed on glycerol containing medium up to 168 h (7). The results from the available literature where xanthan biosynthesis was performed on glycerol containing media show that the applied producing strains did not metabolize all available amount of glycerol during the cultivation of 96 h (15) and 120 h (20), and glycerol conversion was about 50% or less.…”
In this study, the influence of cultivation time on xanthan biosynthesis by
different Xanthomonas strains, reference strain and crucifers? and pepper
leaves? isolates, was examined. Biopolymer was produced by submerged
cultivation of fourteen producing strains on medium with glycerol as a sole
carbon source. Each experiment was performed at a laboratory level under
aerobic conditions at 30?C and 150 rpm for 168 h and 240 h. Bioprocess
efficacy was estimated based on the xanthan quantity and its average
molecular weight that was selected as quality parameter. According to the
obtained results, it can be concluded that all applied strains have a
statistically significant effect on xanthan concentration in medium and on
its average molecular weight, while cultivation time significantly affect
the bioprocess efficacy only when biosynthesis is performed by Xanthomonas
strains isolated from crucifers. Further, when only Xanthomonas strains
isolated from crucifers are observed, statistically obtained data suggest
that the largest amount of the best quality xanthan in applied experimental
conditions can be accomplished by the cultivation of CB strain for 240 h. On
the other hand, when it comes to Xanthomonas strains isolated from pepper
leaves, the highest productivity is shown by PL 2, PL 4 and PL 5 strains
regardless of the cultivation time, while the PL 3 strain was responsible
for the synthesis of biopolymers with the highest average molecular weight.
The results obtained in this study represent valuable information for
development of biotechnological process for xanthan production on glycerol
containing media using new producing strain.
“…Comparing the results obtained in this study (Table 3) with the amount of biopolymer produced by the reference producing strain on the traditionally applied medium with glucose (Bajić et al., 2015), the success of xanthan biosynthesis on the selected wastewater from red wine technology can be estimated in the best way. As the amount of accumulated xanthan in the medium prepared with wastewater generated during fermentation tank washing was 20% higher, it is indisputable that the selected winery effluent represents a very good substitute for standard semisynthetic medium.…”
Production of wines from white and red grapes represents a significant segment of agriculture and food industry because it contributes to improving the economy of both sectors. According to the data reported by the International Organization of Vine and Wine (OIV), since 2000 the world wine production, excluding juices and grape must, is higher than 250 × 10 6 hl/year. With approximately 60% of the produced wine and growing production capacity, the European Union (EU) has a significant presence in the worldwide wine market.
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