Glycerol and Antimicrobial Peptide-Modified Natural Latex for Bacteriostasis of Skin Wounds

Abstract

This work aimed to develop a glycerol antimicrobial peptide natural latex film (NRL-GI-AMP film) for the treatment of skin wound infections. The contents of this work mainly include investigating the effect of adding glycerol (GI) and an antimicrobial peptide (AMP) on the physical and chemical properties of natural latex (NRL) and analyzing the cytocompatibility, bacteriostatic activity, and infected wound healing promotion of the NRL-GI-AMP film. The results showed that the addition of GI resulted in more pores in the internal structure of the NRL film, while the addition of G(LLKK)3L AMP did not change the structure and properties of the NRL film. Compared with that of the NRL film, the infrared spectrum of the NRL-GI-AMP film did not produce new characteristic peaks, indicating that GI and AMP were non-covalently cross-linked with NRL. Addition of 10% GI reduces the toughness of the NRL-GI-AMP film by 62.0%, increases the water vapor transmission rate by 8.95 mg/(cm2·h), and reduces the water absorption and water retention distributions by 33.0 and 24.7%, respectively. AMP in the NRL-GI-AMP film could be released continuously for 40 h, and the release rate was about 45%. The NRL-GI-AMP film showed good biocompatibility and antibacterial activity and promoted the healing of infected wounds. Therefore, the NRL-GI-AP film has potential application in the development of dressings to inhibit skin wound infection and promote wound healing.

Electrochemical Study on the Macro-Cell Corrosion of Pipeline Steel Partially Covered by Different Kinds of Mineral Deposits

“…However, the CaCO 3 powder would aggregate on the surface of carbon steel and become viscous during the experiment. 54−56 The aggregation CaCO 3 would adsorb on the carbon steel surface, 54,57 and its blocking effect for the ions would also be enhanced. Compared to the effect of pH variation, the hindrance of CaCO 3 might play a major role in the corrosion process, resulting in a slightly positive increase in the corrosion potential.…”

Enhancing Healing of Infected Wounds with Glycerin‐Modified Sodium Alginate/Silk Sericin Composite Film Functionalized with Polygonatum sibiricum Polysaccharide‐Capped Silver Nanoparticles

“…A sample (5 μL, 0.5 g of composite film was soaked in 5 mL of PBS solution for 24 h) of the supernatant in which the composite film had been soaked for 24 h was placed in the center of the filter paper. After 14 h of incubation at 37 °C, the diameter of the zone of inhibition was measured [2] …”

“…S. aureus (200 μL of a 10 5 CFU/mL suspension) was inoculated into the wound. After inoculation, the wound was treated with SA/SS, SA/SS/GI, or SA/SS/GI/PSP@AgNP (PSP@AgNP content: 45.7 μg/g) composite films or with PBS [2] . Images of the wound were taken every 2 d.…”

“…After 14 h of incubation at 37 °C, the diameter of the zone of inhibition was measured. [2] Live/Dead Bacterial Staining E. coli and S. aureus were cultured to the logarithmic growth stage. The bacteria were recovered by centrifugation at 4000 rpm for 10 min, and the supernatant was discarded.…”

“…Skin damage can allow pathogenic bacteria, including Escherichia coli and Staphylococcus aureus, to enter the system directly, potentially resulting in serious infections in the wound and posing a threat to human health. [1,2] Although the skin has a limited self-repair ability, the use of wound dressings to promote healing is a commonly used approach. [3,4] Repairpromoting wound dressings are typically three-dimensional and cross-linked hydrophilic polymers that can absorb exudate and provide an ideal environment for wound healing.…”