Glycerol 3-phosphate dehydrogenase regulates heat shock response in Saccharomyces cerevisiae

Pallapati, Anusha R.; Prasad, Shivcharan; Roy, Ipsita

doi:10.1016/j.bbamcr.2022.119238

Cited by 2 publications

(2 citation statements)

References 68 publications

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“…For further confirmation that the HOG MAPK pathway is constitutively active in flocculating cells, we checked the basal level expression of genes ( GPD1 , CTT1 and GRE2 ) for which expression depends on Hog1 phosphorylation by a quantitative real‐time PCR (qRT‐PCR). GPD1 encodes a NAD‐dependent glycerol‐3‐phosphate dehydrogenase which is a key enzyme for glycerol synthesis required for growth under osmotic stress and also regulates the heat shock response [63]. CTT1 encodes a cytosolic catalase T, involved in protection from oxidative damage.…”

Section: Resultsmentioning

confidence: 99%

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Kumawat,

Tomar

2024

The FEBS Journal

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Living organisms are frequently exposed to multiple biotic and abiotic stress forms during their lifetime. Organisms cope with stress conditions by regulating their gene expression programs. In response to different environmental stress conditions, yeast cells activate different tolerance mechanisms, many of which share common signaling pathways. Flocculation is one of the key mechanisms underlying yeast survival under unfavorable environmental conditions, and the Tup1‐Cyc8 corepressor complex is a major regulator of this process. Additionally, yeast cells can utilize different mitogen‐activated protein kinase (MAPK) pathways to modulate gene expression during stress conditions. Here, we show that the high osmolarity glycerol (HOG) MAPK pathway is involved in the regulation of yeast flocculation. We observed that the HOG MAPK pathway was constitutively activated in flocculating cells, and found that the interaction between phosphorylated Hog1 and the FLO genes promoter region increased significantly upon sodium chloride exposure. We found that treatment of cells with cantharidin decreased Hog1 phosphorylation, causing a sharp reduction in the expression of FLO genes and the flocculation phenotype. Similarly, deletion of HOG1 in yeast cells reduced flocculation. Altogether, our results suggest a role for HOG MAPK signaling in the regulation of FLO genes and yeast flocculation.

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Section: Resultsmentioning

confidence: 99%

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Kumawat,

Tomar

2024

The FEBS Journal

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“…A moderately increased temperature can shorten the brewing cycle and provide abundant flavor substances [ 16 , 17 , 18 ]. In the model strain Saccharomyces cerevisiae, studies have shown that it first increases the gene expression of heat–shock proteins (HSPs) via a heat–shock response (HSF) to high temperature stress [ 19 , 20 , 21 ]; the second response is to synthesize functional heat–shock defense substances such as trehalose, xylitol, and cysteine [ 22 , 23 ]; and the third response is to promote superoxide dismutases, catalases, and peroxidases to cope with the oxidative damage caused by heat–induced oxidative stress [ 24 , 25 , 26 ]. It has also been reported that the chromatin of budding yeast polymerizes with each other to form disulfide polymers during a 120 min heat–shock process [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

High Glucose Is a Stimulation Signal of the Salt–Tolerant Yeast Zygosaccharomyces rouxii on Thermoadaptive Growth

Yan,

Xiao,

Liu

et al. 2024

JoF

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The salt–tolerant yeast Zygosaccharomyces rouxii is a typical aroma–producing yeast used in food brewing, but its mechanism of high temperature tolerance is still unclear. In this study, the response mechanism of Z. rouxii to glucose under high temperature stress at 40 °C was explored, based on the total synthetic lowest–nutrient medium. The results of the growth curves and scanning electron microscopy showed that high glucose was necessary for Z. rouxii to restore growth under high temperature stress, with the biomass at 300 g/L of glucose (OD600, 120h = 2.44 ± 0.26) being 8.71 times higher than that at 20 g/L (OD600, 120h = 0.28 ± 0.08). The results of the transcriptome analysis, combined with RT–qPCR, showed that the KEGG analysis of differentially expressed genes was enriched in pathways related to glucose metabolism, and high glucose (300 g/L) could effectively stimulate the gene expression of glucose transporters, trehalose synthesis pathways, and xylitol synthesis pathways under a high temperature, especially the expression of the glucose receptor gene RGT2 (up–regulated 193.7 times at 12 h). The corresponding metabolic characteristics showed that the contents of intracellular metabolites, such as glucose (Cmax, 6h = 6.50 ± 0.12 mg/g DCW), trehalose (Cmax, 8h = 369.00 ± 17.82 μg/g DCW), xylitol (Cmax, 8h = 1.79 ± 0.27 mg/g DCW), and glycerol (Cmax, 8h = 268.10 ± 44.49 μg/g DCW), also increased with time. The accumulation of acetic acid, as the main product of overflow metabolism under high temperature stress (intracellular Cmax, 2h = 126.30 ± 10.96 μg/g DCW; extracellular Cmax, 12h = 499.63 ± 27.16 mg/L), indicated that the downstream glycolysis pathway was active. Compared with the normal physiological concentration of glucose, a high glucose concentration can effectively stimulate the gene expression and metabolism of salt–tolerant Z. rouxii under high–temperature conditions to restore growth. This study helps to deepen the current understanding of the thermoadaptive growth mechanism of salt–tolerant Z. rouxii.

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Glycerol 3-phosphate dehydrogenase regulates heat shock response in Saccharomyces cerevisiae

Cited by 2 publications

References 68 publications

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

High Glucose Is a Stimulation Signal of the Salt–Tolerant Yeast Zygosaccharomyces rouxii on Thermoadaptive Growth

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