Glycerol‐3‐phosphate dehydrogenase is induced by glucocorticoids in hepatocytes and hepatoma cells in vitro

Meyer, Robert D.; Preston, Sheryl L.; McMorris, F. Arthur

doi:10.1002/jcp.1041140209

Cited by 13 publications

(4 citation statements)

References 47 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aliquots of the unfractionated brain homogenates from the first step in the myelin isolation procedure were stored at -70°C for subsequent determinations. A portion of the aliquot was centrifuged at 30,900 g for 1 h, and the supernatant was assayed for GPDH activity, as described by Meyer et al (1983). CNP activity was assayed in unfractionated homogenates, following the procedure of Prohaska et al (1973).…”

Section: Enzyme and Dna Assaysmentioning

confidence: 99%

Adrenalectomy of Rats Results in Hypomyelination of the Central Nervous System

Preston

McMorris

1984

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

The effect of adrenalectomy on CNS myelin accumulation was investigated to determine whether glucocorticoids play a role in regulating myelination. When 14-day-old rats were adrenalectomized and sacrificed 7-8 days later, the amount of bulk-isolated myelin in whole brain, as expressed per gram wet weight of brain or per milligram DNA-phosphate, was reduced to about 75% that of sham-operated controls. Both brain weight and DNA content were unchanged by adrenalectomy. Examination of individual brain regions also revealed decreased amounts of myelin in adrenalectomized animals. Brain glycerol 3-phosphate dehydrogenase specific activity was reduced in adrenalectomized animals to 40-60% that of controls, and serum corticosterone levels were less than 0.6% of control levels. The amount of cerebral myelin in animals adrenalectomized on day 21 and sacrificed 9 days later was not significantly reduced. This suggests a possible role of glucocorticoids during the early period of rapid myelination.

show abstract

Section: Enzyme and Dna Assaysmentioning

confidence: 99%

Adrenalectomy of Rats Results in Hypomyelination of the Central Nervous System

Preston

McMorris

1984

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cytosolic GPDH activity and gene expression are increased during the active phase of glycerol accumulation in rainbow smelt following the natural thermal cycle (12,13), GPDH activity increases in liver of fish subjected to acute decreases in water temperature (6), and GPDH mRNA decreases in fish subjected to acute temperature increase (8). GPDH activity is stimulated in rat hepatoma cell lines and transformed rat hepatocytes after 3 days of in vitro culture with glucocorticoids (14), and GPDH activity and mRNA level are stimulated by glucocorticoids in the rat brain (2). Also, in mouse liver, the GPDH gene has a functional peroxisome proliferator-activated receptor (PPAR) response element in its promoter that is a direct target of PPAR␣ (21).…”

mentioning

confidence: 98%

Low temperature directly activates the initial glycerol antifreeze response in isolated rainbow smelt (Osmerus mordax) liver cells

Clow¹,

Ewart²,

Driedzic³

2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Rainbow smelt (Osmerus mordax) accumulate high levels of glycerol in winter that serve as an antifreeze. Liver glycogen is a source of glycerol during the early stages of glycerol accumulation, whereas dietary glucose and amino acids are essential to maintain rates of glycerol synthesis. We presently report rates of glycerol and glucose production by isolated hepatocytes. Cells from fish held at 0.4 to -1.5 degrees C and incubated at 0.4 degrees C were metabolically quiescent with negligible rates of glycerol or glucose production. Hepatocytes isolated from fish maintained at 8 degrees C and incubated at 8 degrees C produced glucose but not glycerol. Glycerol production was activated in cells isolated from 8 degrees C fish and incubated at 0.4 degrees C without substrate or when glucose, aspartate, or pyruvate was available in the medium. Incubation at 0.4 degrees C without substrate resulted in similar molar rates of glucose and glycerol production in concert with glycogen mobilization. Glycogenolysis and glycerol production were associated with increases in total in vitro activities of glycogen phosphorylase and glycerol-3-phosphate dehydrogenase. Maximal in vitro activities of hexokinase and glucokinase were not influenced by temperature, but high activities of a low-K(m) hexokinase may serve to redirect glycogen-derived glucose to glycolysis as opposed to releasing it from the cells. Rates of glycerol production were not enhanced in cells from fish held at 8 degrees C and incubated at 0.4 degrees C with adrenergic or glucocorticoid stimulation. As such, low temperature alone is sufficient to activate the glycerol production mechanism and results in a shift from glucose to a mix of glucose and glycerol production.

show abstract

“…Interestingly, it has been shown that this enzyme can be induced by glucocorticoids in several mammalian tissues (Meyer et al, 1983). The spot intensity in corticosterone-treated samples was also increased (1.7-fold) compared to controls.…”

Section: Discussionmentioning

confidence: 92%

Analysis of aldosterone-induced differential receptor-independent protein patterns using 2D-electrophoresis and mass spectrometry

Böhmer¹,

Carapito²,

Wilzewski³

et al. 2006

Biological Chemistry

View full text Add to dashboard Cite

In the human body the mineralocorticoid aldosterone is responsible for maintaining water and electrolyte homeostasis and therefore controlling blood pressure. In addition, aldosterone has recently been associated with severe heart failure. Besides receptor-dependent action, the damaging effects of aldosterone may also be partly mediated through non-genomic mechanisms. The present study focuses on the mineralocorticoid receptor-independent action of aldosterone at the protein level. We chose the fission yeast Schizosaccharomyces pombe as a model organism, since this yeast does not contain nuclear steroid receptors, but many genes and regulatory mechanisms that are close to those of mammals. Using 2D-electrophoresis we identified for the first time protein spots affected by aldosterone in a nuclear receptor-free system. Mass spectrometry analysis using MALDI-TOF MS and nanoLC-MS/MS approaches allowed the unambiguous identification of 11 proteins that showed increased or decreased levels, which may represent newly identified players and pathways of aldosterone-induced action. Two proteins with a connection to osmotic regulation (NAD-dependent malic enzyme and glycerol-3-phosphate-dehydrogenase), as well as two proteins involved in the overall organization of the cytoskeleton, vip1 and glyceraldehyde-3-phosphate dehydrogenase, which was also found to be specifically affected by aldosterone in human HCT116 cells, are discussed.

show abstract

Glycerol‐3‐phosphate dehydrogenase is induced by glucocorticoids in hepatocytes and hepatoma cells in vitro

Cited by 13 publications

References 47 publications

Adrenalectomy of Rats Results in Hypomyelination of the Central Nervous System

Adrenalectomy of Rats Results in Hypomyelination of the Central Nervous System

Low temperature directly activates the initial glycerol antifreeze response in isolated rainbow smelt (Osmerus mordax) liver cells

Analysis of aldosterone-induced differential receptor-independent protein patterns using 2D-electrophoresis and mass spectrometry

Contact Info

Product

Resources

About