Glyceraldehyde as an Efficient Chemical Crosslinker Agent for the Formation of Chitosan Hydrogels

Carmona, Pierre; Tasici, Anca M.; Sande, Sverre Arne; Knudsen, Kenneth D.; Nyström, Bo

doi:10.3390/gels7040186

Cited by 8 publications

(4 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect likely occurred because increasing the polymer concentration increased the polymer network packing density, which slowed coating degradation and impeded the release of incorporated pDNA molecules. 56,57 We also found that GTA crosslinking decreased pDNA release for the collagen and acidic gelatin-coated PCL scaffolds, while the GTA-crosslinked basic gelatin-coated PCL scaffolds suspended pDNA release (Figure 4E). We further confirmed the significant influence of GTA concentration on the release of pDNA, particularly in basic gelatin, as opposed to acidic gelatin.…”

Section: Natural Polymer Coating and Crosslinking Concentrations Infl...mentioning

confidence: 65%

MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration

Remy,

Upara,

Ding

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

The fabrication of clinically relevant synthetic bone grafts relies on combining multiple biodegradable biomaterials to create a structure that supports the regeneration of defects while delivering osteogenic biomolecules that enhance regeneration. functions as a potent osteoinductive biomolecule to enhance osteogenic differentiation and bone formation; however, synthetic tissue-engineered bone grafts that sustain the delivery of miR-200c for bone regeneration have not yet been evaluated. In this study, we created novel, multimaterial, synthetic bone grafts from gelatin-coated 3D-printed polycaprolactone (PCL) scaffolds. We attempted to optimize the release of pDNA encoding miR-200c by varying gelatin types, concentrations, and polymer crosslinking materials to improve its functions for bone regeneration. We revealed that by modulating gelatin type, coating material concentration, and polymer crosslinking, we effectively altered the release rates of pDNA encoding miR-200c, which promoted osteogenic differentiation in vitro and bone regeneration in a critical-sized calvarial bone defect animal model. We also demonstrated that crosslinking the gelatin coatings on the PCL scaffolds with low-concentration glutaraldehyde was biocompatible and increased cell attachment. These results strongly indicate the potential use of gelatin-based systems for pDNA encoding microRNA delivery in gene therapy and further demonstrate the effectiveness of miR-200c for enhancing bone regeneration from synthetic bone grafts.

show abstract

Section: Natural Polymer Coating and Crosslinking Concentrations Infl...mentioning

confidence: 65%

MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration

Remy,

Upara,

Ding

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…In the literature, there are some experimental papers ,, on salt-free polyelectrolyte solutions that are consistent with the theoretical prediction of α = 0.5 and β = 1.5. However, there are also other studies ,,, in the literature on salt-free polyelectrolyte solutions where β = 1.5 was never observed, but values of β in the range of 3–4 were reported.…”

Section: Resultsmentioning

confidence: 99%

Interactions in Aqueous Mixtures of Cationic Hydroxyethyl Cellulose and Different Anionic Bile Salts

Tan

Gjerde

Giudice

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

It is known that the reduction of blood cholesterol can be accomplished through foods containing a large number of dietary fibers; this process is partially related to the binding of bile salt to fibers. To gain new insights into the interactions between dietary fibers and bile salts, this study investigates the interactions between cationic hydroxyethyl cellulose (catHEC) and sodium deoxycholate (NaDC) or sodium cholate (NaC), which have a similar structure. Turbidity measurements reveal strong interactions between catHEC and NaDC, and under some conditions, macroscopic phase separation occurs. In contrast, the interactions with NaC are weak. At a catHEC concentration of 2 wt %, incipient phase separation is approached at concentrations of NaC and NaDC of 32.5 and 19.3 mM, respectively. The rheological results show strong interactions and a prominent viscosification effect for the catHEC/NaDC system but only moderate interactions for the catHEC/NaC system. Both cryogenic transmission electron microscopy and small-angle X-ray scattering results display fundamental structural differences between the two systems, which may explain the stronger interactions in the presence of NaDC. The surmise is that the extended structures formed in the presence of NaDC can easily form connections and entanglements in the network.

show abstract

“…Therefore, the gelation time needed to be identified to ensure the formation of a three-dimensional network. Several techniques, such as light scattering (LS) [12,33], beam diffraction [13], and rheology [34][35][36], have been widely used to elucidate the mechanism of material gelation and its molecular structure. In this work, the rheological technique was used to investigate the crosslinking behavior due to its direct correlation with the evolving physical and mechanical properties of the system during the crosslinking process.…”

Section: Uv-induced Sol-to-gel Transition Of Pnipam Solutionmentioning

confidence: 99%

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

Dueramae,

Tanaka,

Shinyashiki

et al. 2023

Gels

View full text Add to dashboard Cite

High-performance properties of interpenetration polymer network (IPN) hydrogels, based on physically crosslinked chitosan (CS) and chemically crosslinked poly(N-isopropylacrylamide) (PNiPAM), were successfully developed. The IPN of CS/PNiPAM is proposed to overcome the limited mechanical properties of the single CS network. In this study, the viscoelastic behaviors of prepared materials in both solution and gel states were extensively examined, considering the UV exposure time and crosslinker concentration as key factors. The effect of these factors on gel formation, hydrogel structures, thermal stabilities of networks, and HeLa cell adhesion were studied sequentially. The sol–gel transition was effectively demonstrated through the scaling law, which agrees well with Winter and Chambon’s theory. By subjecting the CS hydrogel to the process operation in an ethanol solution, its properties can be significantly enhanced with increased crosslinker concentration, including the shear modulus, crosslinking degree, gel strength, and thermal stability in its swollen state. The IPN samples exhibit a smooth and dense surface with irregular pores, allowing for much water absorption. The HeLa cells were adhered to and killed using the CS surface cationic charges and then released through hydrolysis by utilizing the hydrophilic/hydrophobic switchable property or thermo-reversible gelation of the PNiPAM network. The results demonstrated that IPN is a highly attractive candidate for anti-fouling materials.

show abstract

Glyceraldehyde as an Efficient Chemical Crosslinker Agent for the Formation of Chitosan Hydrogels

Cited by 8 publications

References 77 publications

MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration

MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration

Interactions in Aqueous Mixtures of Cationic Hydroxyethyl Cellulose and Different Anionic Bile Salts

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

Contact Info

Product

Resources

About