“…When the cells were 70–80% confluent, they were treated with vehicle control (DMSO), 5 μM Lap, 10 μM Gly, and 5 μM Lap plus 10 μM Gly for 24 h. The cells were scraped and homogenized in cold RIPA buffer supplemented with a protease inhibitor (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Protein separation, membrane blotting, and antibody probing were conducted as previously described [ 26 ] using the following primary antibodies against cyclin B1 (Rb IgG, D5C10) (1:1000), cdk2 (Rabbit mAb, 78B2) (1:1000), p21 (Rabbit mAb, 78B2) (1:1000), p27 (Rabbit mAb, 78B2) (1:1000), β actin (Rabbit mAb, 13E5) (1:1000), or GAPDH (Rb mAb (14C10) (1:1000) (Cell Signaling Technology, Danvers, MA, USA), or against cdk1 (Rabbit mAb, YE324) (1:2000) (Abcam Waltham, Boston, MA, USA). Afterwards, they were then washed and incubated with anti-rabbit secondary antibody.…”