Glycated Plasma Proteins as More Sensitive Markers for Glycemic Control in Type 1 Diabetes

Zhang, Lína; Zhang, Qibin

doi:10.1002/prca.201900104

Cited by 15 publications

(15 citation statements)

References 61 publications

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“…Diabetes, with the basic characteristic of hyperglycaemia, is a chronic metabolic disease and one of the most challenging heterogeneous diseases, which can be briefly divided into type 1 diabetes mellitus (T1DM) and T2DM [1,2]. Type 1 diabetes mellitus is a chronic autoimmune disease that is featured by the destruction of islet b-cells, ultimately leading to insulin deficiency [3,4]. The pathogenesis of T1DM is complex and may be caused by complex interactions between environmental and genetic factors, just like other autoimmune diseases [5].…”

Section: Introductionmentioning

confidence: 99%

miRNA-16-5p inhibits the apoptosis of high glucose-induced pancreatic β cells via targeting of CXCL10: potential biomarkers in type 1 diabetes mellitus

Gao

Zhao²

2020

Endokrynologia Polska

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Introduction:We aimed to elucidate the relationship between CXC chemokine ligand 10 (CXCL10) and miR-16-5p, and their functions on the biological behaviour of type 1 diabetes mellitus (T1DM). Material and methods: The GSE72492 dataset from the GEO database was used to analyse gene expression. We discovered that CXCL10 was highly expressed in T1DM patients. The up-stream miRNA was predicted by Targetscan website. Low glucose (2.8 mmol/L) and high glucose (HG, 16.7 mmol/L) were utilised to treat b-TC-tet (pancreatic b cell) cells to form the model. The direct interaction between miR-16-5p and CXCL10 was verified by a dual-luciferase reporter assay. Real-time quantitative PCR (qRT-PCR) and western blotting analyses were used to detect RNA and protein expression. CCK8 and flow cytometry were used to detect cell proliferation and apoptosis. Results: We discovered that CXCL10 was highly expressed in T1DM patients. MiR-16-5p, which was lowly expressed in T1DM patients, was verified the upstream regulatory miRNA of CXCL10. The facilitating influence of miR-16-5p up-regulation on the proliferation of HG-induced b-TC-tet cells was reversed by CXCL10 over-expression, while the knockdown results were opposite. More importantly, the restraining impact of miR-16-5p high expression on the apoptosis of HG-induced b-TC-tet cells was accelerated by CXCL10 over-expression. Correspondingly, the level of Bcl-2 was enhanced while the levels of Bax and Cleaved Caspase-3 were lowered by miR-16-5p mimic, which were reversed by CXCL10 over-expression in HG-treated b-TC-tet cells. Conclusions: Our data offered evidence that miR-16-5p implicated in T1DM cell proliferation and apoptosis through targeting CXCL10, which might provide novel therapeutic information for T1DM. (Endokrynol Pol 2020; 71 (5): 404-410

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Section: Introductionmentioning

confidence: 99%

miRNA-16-5p inhibits the apoptosis of high glucose-induced pancreatic β cells via targeting of CXCL10: potential biomarkers in type 1 diabetes mellitus

Gao

Zhao²

2020

Endokrynologia Polska

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“…This helps to optimize the diagnostic method and to predict related complications. The biomarkers research regarding diabetes nephropathy, a serious complication of diabetes, has made great progress [10,11], however, study of early diagnosis of T2DM is still limited [12]. To a certain extent, this is due to that the presence of severe metabolic disorders and signs of microvascular damage in the stage of diabetic complications help in the selection of markers; while slight changes in blood glucose and other metabolites in the early stages of diabetes are not likely to be discovered by epidemiological studies.…”

Section: Introductionmentioning

confidence: 99%

Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats

Wang

et al. 2020

Nutr Metab (Lond)

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Background: Early diagnosis of type 2 diabetes mellitus (T2DM) is still difficult. Screening of plasma biomarkers has great significance of optimizing diagnosis and predicting the complications of T2DM. Methods: We used a special diet, Purina #5008, to induce diabetes in Zucker leptin receptor gene-deficient rats (fa/ fa) to establish Zucker diabetic fatty (ZDF) rats, simulating the early stage of T2DM. The differentially expressed proteins (DEP) and lipids (DEL), as potential biomarkers, were screened to compare the plasma expression levels in ZDF rats and their basic diet-fed wild-type controls (fa/+) by Tandem Mass Tags (TMT) and liquid chromatographytandem mass spectrometry. Results: These two groups had different plasma proteins and lipids profiles consisting of 84 DEPs and, 179 DELs identified in the positive ion mode and 178 DELs in the negative ion mode, respectively. Enrichment analysis of these different indicators showed that oxidative stress, insulin resistance and metabolic disorders of glycan and lipid played an important role in generating the difference. Some markers can be used as candidate biomarkers in prediction and treatments of T2DM, such as ceruloplasmin, apolipoprotein C-I, apolipoprotein C-II and apolipoprotein C-IV. Conclusion: These plasma differences help to optimize the diagnosis and predict the complications of T2DM, although this remains to be verified in the crowd. Trace elements related-metalloproteins, such as ceruloplasmin, and lipid metabolism and transport-related apolipoprotein C are expected to be candidate biomarkers of T2DM and should be given more attention.

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“…Another study identi ed higher concentrations of glycated complement C4-A in patients with T1DM 37 .…”

Section: Discussionmentioning

confidence: 99%

Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes

Gutaj

Matysiak

Matuszewska

et al. 2021

Preprint

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Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 diabetic women and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation.

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Glycated Plasma Proteins as More Sensitive Markers for Glycemic Control in Type 1 Diabetes

Cited by 15 publications

References 61 publications

miRNA-16-5p inhibits the apoptosis of high glucose-induced pancreatic β cells via targeting of CXCL10: potential biomarkers in type 1 diabetes mellitus

miRNA-16-5p inhibits the apoptosis of high glucose-induced pancreatic β cells via targeting of CXCL10: potential biomarkers in type 1 diabetes mellitus

Metalloproteins and apolipoprotein C: candidate plasma biomarkers of T2DM screened by comparative proteomics and lipidomics in ZDF rats

Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes

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