Glycans and Carbohydrate-Binding/Transforming Proteins in Axon Physiology

Abad‐Rodríguez, José; Brocca, M. E.; Higuero, Alonso M.

doi:10.1007/978-3-031-12390-0_7

Cited by 3 publications

(3 citation statements)

References 206 publications

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“…Intriguingly, functional predictions arising from this investigation have unveiled a significant enrichment of axon guidance pathways in miRNAs closely intertwined with the gut microbiota. Of note, glycans play pivotal roles in transmitting axonal signals, and enzymes responsible for glycan modifications finely regulate the information conveyed to axons, which can subsequently be interpreted by glycan-binding proteins ( Sakamoto et al, 2021 ; Abad-Rodríguez et al, 2023 ). Enhanced glycan production may, in turn, augment the recruitment of certain bacteria ( Kudelka et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation

Yao,

Yan,

et al. 2024

Front. Microbiol.

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While dysbiosis within the intestinal ecosystem has been associated with functional constipation (FC), the mechanisms underlying the interactions between FC and the microbiome remain poorly elucidated. Recent investigations suggested that host microRNAs (miRNAs) can modulate bacterial growth and influence the composition of the gut microbiome. To explore the connection between gut microbiota and fecal miRNAs in FC patients, we initially employed 16S rRNA sequencing to assess the gut microbial landscape in 30 FC patients and 30 healthy controls (HCs). The α-diversity within the FC group exhibited some alterations, and the β-diversity significantly differed, signifying distinctive variations in gut microbiota composition between FC patients and HCs. Subsequently, we identified 44 differentially expressed (DE) miRNAs in feces from FC patients and HCs. Through correlation analysis between DE miRNAs and FC-associated microbiota, we detected an interaction involving nine DE miRNAs (miR-205-5p, miR-493-5p, miR-215-5p, miR-184, miR-378c, miR-335-5p, miR-514a-3p, miR-141-3p, and miR-34c-5p) with seven bacterial genera (Oscillibacter, Escherichia.Shigella, UCG.002, Lachnospiraceae_NK4A136_group, Lachnospiraceae_UCG.010, Eubacterium_ruminantium_group and Megamonas), as evidenced by a co-occurrence network. Further, a comprehensive panel of seven diagnostic biomarkers (Oscillibacter, Escherichia.Shigella, UCG.002, miR-205-5p, miR-493-5p, miR-215-5p, and Lachnospiraceae_NK4A136_group) demonstrated robust discriminatory capacity in predicting FC status when integrated into a random forest model (AUC = 0.832, 95% CI: 65.73–98.88). Microbiomes correlating with DE miRNAs exhibited enrichment in distinct predicted metabolic categories. Moreover, miRNAs correlated with FC-associated bacteria were found to be enriched in signaling pathways linked to colonic contractility, including Axon guidance, PI3K-Akt signaling pathway, MAPK signaling pathway, and Hippo signaling pathway. Our study offers a comprehensive insight into the global relationship between microbiota and fecal miRNAs in the context of FC, presenting potential targets for further experimental validation and therapeutic interventions.

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Section: Discussionmentioning

confidence: 99%

Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation

Yao,

Yan,

et al. 2024

Front. Microbiol.

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“…The cell ion channels are complex protein structures with molecular systems of opening, closing, selectivity, inactivation, and regulatory functions. Ion channels are of special importance in excitable cells of nervous system [20]. It is known that ion channels provide their main properties, e.g., of excitable cells: formation of the resting membrane potential (MPP), action potential (AP), and others [21].…”

Section: Electron Transport and Ion Channelsmentioning

confidence: 99%

Application of fluorescent potential-sensitive probes for the study of living blood and marrow cells

Parkhomenko¹,

Galibin²,

Tomson³

2023

CTT

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Usage of membrane potential-sensitive probes is of great importance for assessing both the viability and functional integrity of the cells and their structural components (mitochondria, nuclei, cytoplasmic membranes, ion channels). Potential advantage of this approach includes studies of native viable cells in order to assess functional state of donor hematopoietic cells before transplantation as well as upon their storage and cultivation. These staining tools allow to assess the state of cellular bioenergetics, i.e., the balance between production and consumption of energy in living cells. The production of energy in mitochondrial structures ensures the cell viability, whereas its impairment leads to the development of different disorders and aging. The purpose of this literature review is to demonstrate the opportunities of using potential-sensitive probes to assess the viability of blood system cells and to study the state of their energy potential.

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“…Ионные каналы клетки представляют собой сложные белковые структуры с молекулярными системами открытия, закрытия, селективности, инактивации и регуляции. Особое значение ионные каналы имеют в возбудимых клетках [20]. Ионные каналы обеспечивают основные свойства возбудимых клеток: формирование мембранного потенциала покоя, потенциала действия (ПД), межклеточную сигнализацию, регуляцию освобождения медиатора, генерацию постсинаптических потенциалов, активную и пассивную деполяризацию, генерацию рецепторных потенциалов в рецепторных клетках и др.…”

Section: транспорт электронов и ионные каналыunclassified

Noninvasive method of monitoring the functional state of living cells

Parkhomenko,

Galibin,

Tomson

2023

The Scientific Notes of the Pavlov university

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The article is devoted to the generalization of information on the use of potential-sensitive probes (PSP) to assess the viability and functional state of an entire cell. Usage of membrane PSP is of great importance for assessing both the viability and functional integrity of the cells and their structural components (mitochondria, nuclei, cytoplasmic membranes, ion channels). Potential advantage of this approach includes studies of native viable cells in order to assess functional state of donor hematopoietic cells before transplantation as well as upon their storage and cultivation. These staining tools allow to assess the state of cellular bioenergetics, i.e., the balance between production and consumption of energy in living cells. The production of energy in mitochondrial structures ensures the cell viability, whereas its impairment leads to the development of different disorders and aging. In clinical medicine, this method can be used to assess the condition of donor cells before their transplantation, primarily in oncohematology, the treatment of patients with severe ischemic myocardial lesions.The purpose of the work: to study the results of the research of the use of PSP to assess the energy potential and viability of cells.

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Glycans and Carbohydrate-Binding/Transforming Proteins in Axon Physiology

Cited by 3 publications

References 206 publications

Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation

Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation

Application of fluorescent potential-sensitive probes for the study of living blood and marrow cells

Noninvasive method of monitoring the functional state of living cells

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