Glycan microarray: Toward drug discovery and development

Muthana, Saddam

doi:10.1016/b978-0-12-816675-8.00006-3

Cited by 6 publications

(3 citation statements)

References 102 publications

(75 reference statements)

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“…Through these representative examples, it is obvious to draw the conclusion that highly efficient CuAAC conjugation chemistry is a powerful high-throughput polar immobilization tool for the successful fabrication of surface-based glycan microarrays and monolayer-assisted lectin–sugar interaction assays for the recognition of particular proteins of great pathological significance. In addition to their significant role in carbohydrate–protein interactions, glycan microarrays have also emerged as a powerful tool for study of the interaction of carbohydrates with a variety of other glycan binding molecules, including antibodies, cells, bacteria, and viruses. ,− All together the merits associated with glycan microarrays make them an ideal tool to explore in the growing area of biomedicines, particularly to assist the discovery of novel diagnostic tools for disease detection, mainly cancer diagnosis, prognosis, and therapeutics, such as enzyme inhibitors, autoimmune diseases, infectious diseases, and vaccine development. Although research on glycan microarrays is rather difficult and challenging and only a few groups are working worldwide on this theme, beyond doubt, there are tremendous opportunities available.…”

Section: Application Of Cuaac Bioorthogonal Conjugation In Chemical B...mentioning

confidence: 99%

Cu(I)-Catalyzed Click Chemistry in Glycoscience and Their Diverse Applications

et al. 2021

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Copper(I)-catalyzed 1,3-dipolar cycloaddition between organic azides and terminal alkynes, commonly known as CuAAC or click chemistry, has been identified as one of the most successful, versatile, reliable, and modular strategies for the rapid and regioselective construction of 1,4-disubstituted 1,2,3-triazoles as diversely functionalized molecules. Carbohydrates, an integral part of living cells, have several fascinating features, including their structural diversity, biocompatibility, bioavailability, hydrophilicity, and superior ADME properties with minimal toxicity, which support increased demand to explore them as versatile scaffolds for easy access to diverse glycohybrids and well-defined glycoconjugates for complete chemical, biochemical, and pharmacological investigations. This review highlights the successful development of CuAAC or click chemistry in emerging areas of glycoscience, including the synthesis of triazole appended carbohydrate-containing molecular architectures (mainly glycohybrids, glycoconjugates, glycopolymers, glycopeptides, glycoproteins, glycolipids, glycoclusters, and glycodendrimers through regioselective triazole forming modular and bio-orthogonal coupling protocols). It discusses the widespread applications of these glycoproducts as enzyme inhibitors in drug discovery and development, sensing, gelation, chelation, glycosylation, and catalysis. This review also covers the impact of click chemistry and provides future perspectives on its role in various emerging disciplines of science and technology.

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Section: Application Of Cuaac Bioorthogonal Conjugation In Chemical B...mentioning

confidence: 99%

Cu(I)-Catalyzed Click Chemistry in Glycoscience and Their Diverse Applications

et al. 2021

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“…The first reported glycan microarray in the year 2002 [ 175 , 176 ] studied GBP events using a small amount of a glycan sample [ 28 , 29 , 177 ]. Later years witnessed the use of glycan microarrays in assessing glycan-processing enzyme characterization, the discovery of functional glycans, drug discovery, studying weak carbohydrate interactions, and pathogen diagnosis [ 76 , 178 , 179 , 180 ]. The immobilization of glycans on a solid surface is generally performed after the target glycan is modified with a reactive functional group (NHS, amines, alkenes, carbonyls, and thiols).…”

Section: Application Of Microarraysmentioning

confidence: 99%

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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“…Glycan microarrays are valuable tools for identifying serum antibodies directed against glycan biomarkers as part of the diagnosis of infectious diseases, cancer and autoimmune diseases, as well as for monitoring immune responses to carbohydrate-based synthetic vaccines. 279 Specifically, altered glycosylation on cell surfaces is one of the hallmarks of cancer, and patients suffering from this disease produce anti-glycan antibodies against tumor-associated carbohydrate antigens. 280,281 In addition, patients suffering from infectious and autoimmune diseases also generate anti-glycan antibodies against unique disease-related glycans.…”

Section: Profiling Of Anti-glycan Antibodies In Seramentioning

confidence: 99%