Glycan-based DC-SIGN targeting vaccines to enhance antigen cross-presentation

Lipopolysaccharide Engineering in Neisseria meningitidis

“…Targeting to DC-SIGN could be used to further improve LPS adjuvant properties, as it has been demonstrated to lead to a Th1 bias in T cells activated by N. meningitidis-stimulated DC (26). Simultaneous triggering of DC-SIGN and TLR4 could result in cross-presentation and enhanced CD8 ϩ in addition to CD4 ϩ T cell responses (32). It is therefore important that MS analysis of the lgtB Ϫ pagL ϩ LPS showed only a single oligosac- molecular ion regions of the mass spectra of untreated LPS (A), LPS that had been incubated in neutral 5% TEA at RT for 3 h (B), and LPS that had been incubated in 5% alkaline triethylamine at 45°C for 3 h (C) are presented.…”

In vivo targeting of porcine reproductive and respiratory syndrome virus antigen through porcine DC-SIGN to dendritic cells elicits antigen-specific CD4T cell immunity in pigs

“…The pig dermal layer is rich in dendritic cells that can be phenotypically and functionally divided into multiple subsets, some of which express pDC-SIGN [22]. We delivered the antigen to the dermis region through the efficient transdermal route [34] along with MPLA adjuvant to induce maturation of pDC-SIGN expressing DCs [35,36].…”

Glycoproteomic Analysis of Seven Major Allergenic Proteins Reveals Novel Post-translational Modifications

“…APCs are equipped with an array of glycan receptors and targeting such receptors has been shown to induce tolerance (15,(55)(56)(57)(58)(59)(60)(61)(62). The idea that carbohydrates are able to boost and shape the immune response has been tested previously in numerous studies, systematically reviewed in (63).…”