Glyburide Regulates UCP1 Expression in Adipocytes Independent of KATP Channel Blockade

Qiu, Yan; Yang, Yuanyuan; Wei, Yuda; Liu, Xiaojian; Feng, Zhuanghui; Zeng, Xuwen; Chen, Yanhao; Liu, Yan; Zhao, Yongxu; Chen, Lanlan; Luo, Lijun; Ding, Qiurong

doi:10.1016/j.isci.2020.101446

Cited by 7 publications

(8 citation statements)

References 37 publications

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“…Eight drugs were chosen, including two antidiabetic drugs (Met and Gli), three antioxidants (NAC, Xan, and Ant), two anti-inflammatory drugs (Chl and Ofl), and Pol, an inhibitor of advanced glycosylation end products (AGEs) . The exact antidiabetic mechanisms of Met and Gli have not been fully disclosed, but these two drugs have been considered to undergo different pathways. , Antioxidants and anti-inflammatory drugs were employed because of the elevated oxidative stress during DC pathogenesis, as well as the tight inflammation association . Meanwhile, AGEs are generated from nonenzymatic glycation between proteins and excess glucose or pentose and are regarded as taking charge of diabetic vascular complications .…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues

Guo,

Li,

Lian

et al. 2023

ACS Sens.

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Diabetic cataract (DC) surgery carries risks such as slow wound healing, macular edema, and progression of retinopathy and is faced with a deficiency of effective drugs. In this context, we proposed a protocol to evaluate the drug's efficacy using lipid droplets (LDs) as the marker. For this purpose, a fluorescent probe PTZ-LD for LDs detection is developed based on the phenothiazine unit. The probe displays polarity-dependent emission variations, i.e., lower polarity leading to stronger intensity. Especially, the probe exhibits photostability superior to that of Nile Red, a commercial LDs staining dye. Using the probe, the formation of LDs in DC-modeled human lens epithelial (HLE) cells is validated, and the interplay of LDs−LDs and LDs-others are investigated. Unexpectedly, lipid transfer between LDs is visualized. Moreover, the therapeutic efficacy of various drugs in DC-modeled HLE cells is assessed. Ultimately, more LDs were found in lens epithelial tissues from DC patients than in cataract tissues for the first time. We anticipate that this work can attract more attention to the important roles of LDs during DC progression.

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Section: Resultsmentioning

confidence: 99%

Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues

Guo,

Li,

Lian

et al. 2023

ACS Sens.

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“…Moreover, thermogenesis could also explain the effects derived from M. alba L. administration. Specifically, UCP1 protein is considered a major regulator of adipose energy expenditure and metabolic homeostasis through thermogenesis activation [ 53 , 54 ]. UCP1 uncouples the proton motive force generated in the mitochondria from ATP production to produce heat, thus increasing the energy expenditure.…”

Section: Discussionmentioning

confidence: 99%

The Prebiotic Effects of an Extract with Antioxidant Properties from Morus alba L. Contribute to Ameliorate High-Fat Diet-Induced Obesity in Mice

et al. 2023

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Obesity is a global health issue, in which modifications in gut microbiota composition have a key role. Different therapeutic strategies are being developed in combination with diet and exercise, including the use of plant extracts, such as those obtained from Morus alba L. leaves. Recent studies have revealed their anti-inflammatory and antioxidant properties. The aim of the present work was to evaluate whether the beneficial effects of M. alba L. leaf extract in high-fat diet-induced obesity in mice is correlated with its impact on gut microbiota. The extract reduced body weight gain and attenuated lipid accumulation, as well as increased glucose sensitivity. These effects were associated with an amelioration of the obesity-associated inflammatory status, most probably due to the described antioxidant properties of the extract. Moreover, M. alba L. leaf extract mitigated gut dysbiosis, which was evidenced by the restoration of the Firmicutes/Bacteroidota ratio and the decrease in plasma lipopolysaccharide (LPS) levels. Specifically, the extract administration reduced Alistipes and increased Faecalibaculum abundance, these effects being correlated with the beneficial effects exerted by the extract on the obesity-associated inflammation. In conclusion, anti-obesogenic effects of M. alba L. leaf extract may be mediated through the amelioration of gut dysbiosis.

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“…The white preadipocytes provided by Dr. Qiurong Ding, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences [ 23 ], Cultured in a primary medium composed of high glucose DMEM, 20 % FBS and 1 % penicillin / Streptomycin at 37 °C in 5 % CO2. The differentiation induction medium was prepared as follows: 10 % FBS + DMEM, 5 µg/mL insulin, 1000nM dexamethasone, 0.5mM isobutyl-1-methylxanthine (IBMX) and 1µM rosiglitazone.…”

Section: Methodsmentioning

confidence: 99%

miR-410-3P inhibits adipocyte differentiation by targeting IRS-1 in cancer-associated cachexia patients

et al. 2021

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Backgrounds Cancer-associated cachexia (CAC) is a metabolic syndrome characterized by progressive depletion of adipose and muscle tissue that cannot be corrected by conventional nutritional therapy. Adipose tissue, an important form of energy storage, exhibits marked loss in the early stages of CAC, which affects quality of life and efficacy of chemotherapy. MicroRNAs (miRNAs) are a class of noncoding RNAs that widely exist in all kinds of eukaryotic cells and play regulatory roles in various biological processes. However, the role of miRNAs in adipose metabolism in CAC has rarely been reported. This study attempted to identify important miRNAs in adipose metabolism in CAC and explore their mechanism to identify a new predictive marker or therapeutic target for CAC-related adipose tissue loss (CAL). Methods In this study, miRNA sequencing was firstly used to identify differentially expressed miRNAs related to CAL and the reliability of the conclusions was verified in large population samples. Furthermore, functional experiments were performed by up and down regulating miR-410-3p in adipocytes. The binding of miR-410-3p to Insulin Receptor Substrate 1 (IRS-1) was verified by Luciferase reporter assay and functional experiments of IRS-1 were performed in adipocytes. Finally, the expression of miR-410-3p in serum exosomes was detected. Results miR-410-3p was selected as differentially expressed miRNA through screening and validation. Adipogenesis was suppressed in miR-410-3p upregulation experiment and increased in downregulation experiment. Luciferase reporter assay showed that miR-410-3p binds to 3′ non-coding region of IRS-1 and represses its expression and ultimately inhibits adipogenesis. miR-410-3p was highly expressed in serum exosomes of CAC patients, which was consistent with results in adipose tissue. Conclusions The expression of miR-410-3p was higher in subcutaneous adipose tissues and serum exosomes of CAC patients, which significantly inhibits adipogenesis and lipid accumulation. The study shows that miR-410-3p could downregulate IRS-1 and downstream adipose differentiation factors including C/EBP-a and PPAR-γ by targeting 3′ noncoding region.

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Glyburide Regulates UCP1 Expression in Adipocytes Independent of KATP Channel Blockade

Cited by 7 publications

References 37 publications

Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues

Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues

The Prebiotic Effects of an Extract with Antioxidant Properties from Morus alba L. Contribute to Ameliorate High-Fat Diet-Induced Obesity in Mice

miR-410-3P inhibits adipocyte differentiation by targeting IRS-1 in cancer-associated cachexia patients

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