Glutenase and collagenase activities of wheat cysteine protease Triticain-α: Feasibility for enzymatic therapy assays

Savvateeva, Lyudmila V.; Gorokhovets, Neonila V.; Макаров, Владимир Александрович; Serebryakova, Marina V.; Solovyev, Andrey G.; Morozov, Sergey Y.; Reddy, V. Prakash; Zernii, Evgeni Yu; Zamyatnin, Andrey A.; Aliev, Gjumrakch

doi:10.1016/j.biocel.2015.03.001

Cited by 39 publications

(26 citation statements)

References 44 publications

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“…Thus, it has been proposed that exogenous enzymes can be employed for additional enzyme supplement therapy to promote the complete digestion of cereal proteins, and thus destroy T-cell gluten epitopes, in particular [129,130]. A number of peptidases possessing glutenase activities were isolated from germinating cereals ( Hordeum vulgare L., Triticum aestivum L.), bacteria ( Flavobacterium meningosepticum , Sphingomonas capsulate , Myxococcus xanthus ), fungi ( Aspergillus niger , Aspergillus oryzae ), and stored-product pest yellow mealworm ( Tenebrio molitor ) [131,132,133,134,135]. One of them is ALV003 enzyme—modified recombinant EP-B2 enzyme from barley, and prolyl endopeptidase from bacteria Sphingomonas capsulate —was shown to be effective in vitro and in vivo, non-toxic and without allergic reactions [136,137].…”

Section: Gluten Detoxification Strategiesmentioning

confidence: 99%

Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities

2016

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Theterm gluten intolerance may refer to three types of human disorders: autoimmune celiac disease (CD), allergy to wheat and non-celiac gluten sensitivity (NCGS). Gluten is a mixture of prolamin proteins present mostly in wheat, but also in barley, rye and oat. Gluten can be subdivided into three major groups: S-rich, S-poor and high molecular weight proteins. Prolamins within the groups possess similar structures and properties. All gluten proteins are evolutionarily connected and share the same ancestral origin. Gluten proteins are highly resistant to hydrolysis mediated by proteases of the human gastrointestinal tract. It results in emergence of pathogenic peptides, which cause CD and allergy in genetically predisposed people. There is a hierarchy of peptide toxicity and peptide recognition by T cells. Nowadays, there are several ways to detoxify gluten peptides: the most common is gluten-free diet (GFD), which has proved its effectiveness; prevention programs, enzymatic therapy, correction of gluten pathogenicity pathways and genetically modified grains with reduced immunotoxicity. A deep understanding of gluten intolerance underlying mechanisms and detailed knowledge of gluten properties may lead to the emergence of novel effective approaches for treatment of gluten-related disorders.

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Section: Gluten Detoxification Strategiesmentioning

confidence: 99%

Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities

2016

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“…According to the SDS-PAGE, the molecular weight protein of protein/peptides ranged from 3 kDa up to 100 kDa; with most within the range from 20 to 65 kDa. SDS profiles of purified bovine collagen show three main bands which correspond to collagen subunits of 114 and 130 kDa molecular weight (Savvateeva, et al, 2015). The absence of such larger proteins supports the fact that proteins were hydrolysed in the rendering process.…”

Section: Resultsmentioning

confidence: 70%

Novel “gel demineralizing” method for protein recovery from fat rendering waste stream based on its gelling properties

2018

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“…2). PE-Peps were found in archeal, bacterial and eukaryotic species and exhibited similar activities against the reference substrates (Savvateeva et al 2015; Venalainen et al 2004). PE-Pep from F. meningosepticum has been well studied for its activity, specificity and stability under gastrointestinal conditions (Shan et al 2004).…”

Section: Resultsmentioning

confidence: 80%

Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac disease

Osorio

Wen

Mejías

et al. 2018

Funct Integr Genomics

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Ubiquitous nature of prolamin proteins dubbed gluten from wheat and allied cereals imposes a major challenge in the treatment of celiac disease, an autoimmune disorder with no known treatment other than abstinence diet. Administration of hydrolytic glutenases as food supplement is an alternative to deliver the therapeutic agents directly to the small intestine, where sensitization of immune system and downstream reactions take place. The aim of the present research was to evaluate the capacity of wheat grain to express and store hydrolytic enzymes capable of gluten detoxification. For this purpose, wheat scutellar calli were biolistically transformed to generate plants expressing a combination of glutenase genes for prolamin detoxification. Digestion of prolamins with barley endoprotease B2 (EP-HvB2) combined with Flavobacterium meningosepticum prolyl endopeptidase (PE-FmPep) or Pyrococcus furiosus prolyl endopeptidase (PE-PfuPep) significantly reduced (up to 67%) the amount of the indigestible gluten peptides of all prolamin families tested. Seven of the 168 generated lines showed inheritance of transgene to the T generation. Reversed phase high-performance liquid chromatography of gluten extracts under simulated gastrointestinal conditions allowed the identification of five T lines that contained significantly reduced amounts of immunogenic, celiac disease-provoking gliadin peptides. These findings were complemented by the R5 ELISA test results where up to 72% reduction was observed in the content of immunogenic peptides. The developed wheat genotypes open new horizons for treating celiac disease by an intraluminal enzyme therapy without compromising their agronomical performance.

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Glutenase and collagenase activities of wheat cysteine protease Triticain-α: Feasibility for enzymatic therapy assays

Cited by 39 publications

References 44 publications

Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities

Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities

Novel “gel demineralizing” method for protein recovery from fat rendering waste stream based on its gelling properties

Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac disease

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