Gluten hydrolase gene silencing using RNAi and its effect on the Sunn pest growth and development

Amiri, Azam; Bandani, Alireza

doi:10.1007/s12600-020-00821-8

Cited by 5 publications

(1 citation statement)

References 61 publications

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“…Other studies have reported similar trends for the different insect developmental stages. For example, in the nymphal stage of the Sunn pest Eurygaster integriceps Puton (Hemiptera: Scutelleridae), gene silencing levels were greater in the nymph than for adults ( Amiri and Bandani, 2020 ). The spotted wing fruit fly Drosophila suzukii (Matsumuru, 1937) larval stage also exhibited higher levels of knockdown across multiple genes, where gene silencing was documented at 32%–42% and 19%–24%, respectively, in larval and adult stages, and mortality was 10%–23% in adults, compared to 22%–42% in larvae ( Taning et al, 2016b ).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of environmental RNAi in potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Psylloidea: Triozidae)

Mondal

Carver²,

Brown³

2022

Front. Physiol.

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RNA interference (RNAi) has potential to become a major tool for integrated management of insect pests of agricultural crops based on sequence-specificity and low doses of rapidly biodegradable dsRNA. Deploying ‘environmental RNAi’ for control of insect vectors of plant pathogens is of increasing interest for combatting emerging plant diseases. Hemipteran insect vectors, including psyllids, are vascular feeders, making their development difficult to control specifically by targeting with pesticidal chemistries. Psyllids transmit “Candidatus Liberibacter solanacearum” the causal organism of potato zebra chip and tomato vein greening diseases, transmitted, respectively, by the potato or tomato psyllid (PoP). Until now, the optimal effective concentration(s) of double-stranded RNA (dsRNA) required for significant gene knockdown and RNAi persistence in PoP have not been determined. The objective of this study was to optimize RNAi in young PoP adults and 3rd instars for screening by oral delivery of dsRNAs. The minimal effective dsRNA concentrations required for robust knockdown and persistence were evaluated by delivering seven concentrations spanning 0.1 ng/μL to 500 ng/μL over post ingestion-access periods (IAP) ranging from 48 h to 12 days. The PoP gene candidates evaluated as targets were vacuolar ATPase subunit A, clathrin heavy chain, and non-fermenting protein 7, which were evaluated for knockdown by qPCR amplification. The minimum and/or the second most effective dsRNA concentration resulting in effective levels of gene knockdown was 100 ng/μL for all three targets. Higher concentrations did not yield further knockdown, indicating potential RISC saturation at the higher doses. Gene silencing post-IAP of 100 ng/μL dsRNA persisted for 3–5 days in adults and nymphs, with the PoP 3rd instar, followed by teneral and mature adults, respectively, exhibiting the most robust RNAi-response.

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Section: Discussionmentioning

confidence: 99%