Gluten Free Wheat: Are We There?

García-Molina, María Dolores; Giménez, María J.; Sánchez-León, Susana; Barro, Francisco

doi:10.3390/nu11030487

Cited by 52 publications

(27 citation statements)

References 62 publications

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“…Aside from the amazing potential for gene editing as a diagnostic tool and perhaps even a treatment for many debilitating diseases (Ortiz-Virumbrales et al 2017;Reczek et al 2017;Zabinyakov et al 2017), the potential possible uses of this technology in plants and livestock animals is similarly impressive (Lamas-Toranzo et al 2017). Creation of disease-resistant and less allergenic food crops (Hummel et al 2018;García-Molina et al 2019) with enhanced nutrient profiles (Wang et al 2019) is now within our reach; the future implications for feeding a progressively larger world population are immense.…”

Section: Discussionmentioning

confidence: 99%

Gene editing in plants: assessing the variables through a simplified case study

Shockey

2020

Plant Mol Biol

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Key message Multiple variables that control the relative levels of successful heritable plant genome editing were addressed using simple case studies in Arabidopsis thaliana. Abstract The recent advent of genome editing technologies (especially CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats) has revolutionized various fields of scientific research. The process is much more specific than previous mutagenic processes and allows for targeting of nearly any gene of interest for the creation of loss-of-function mutations and many other types of editing, including gene-replacement and gene activation. However, not all CRISPR construct designs are successful, due to several factors, including differences in the strength and cell-or tissue-type specificity of the regulatory elements used to express the Cas9 (CRISPR Associated protein 9) DNA nuclease and single guide RNA components, and differences in the relative editing efficiency at different target areas within a given gene. Here we compare the levels of editing created in Arabidopsis thaliana by CRISPR constructs containing either different promoters, or altered target sites with varied levels of guanine-cytosine base content. Additionally, nuclease activity at sites targeted by imperfectly matched single guide RNAs was observed, suggesting that while the primary goal of most CRISPR construct designs is to achieve rapid, robust, heritable gene editing, the formation of unintended mutations at other genomic loci must be carefully monitored.Plant Molecular Biology (2020) 103:75-89Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Discussionmentioning

confidence: 99%

Gene editing in plants: assessing the variables through a simplified case study

Shockey

2020

Plant Mol Biol

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“…Specific amino acid substitutions in a gliadin epitope can abolish its immunogenicity (38) whilst having no effect on gene expression and thus on bread dough quality. With new developments in gene editing being published almost monthly, these options may become available soon, meaning that the goal of editing the wheat gluten genes at the epitope level into CDsafe wheat lines may be realized (26). Base editors are very promising.…”

Section: Technical Considerations For Cd-hypoimmunogenic Glutenmentioning

confidence: 99%

CRISPR/Cas9 Gene Editing of Gluten in Wheat to Reduce Gluten Content and Exposure—Reviewing Methods to Screen for Coeliac Safety

et al. 2020

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“…It was developed by combining existing induced recessive mutations through breeding (140). This strategy is very difficult to implement in bread wheat as it has three genomes and one does not want to remove all gluten genes (141), but other approaches may be successful (142,143). The general applicability of these breeding approaches needs to be discussed, since only gluten endows the dough with the desired properties for bread making.…”

Section: Removing Gluten From Wheatmentioning

confidence: 99%

Recent Progress and Recommendations on Celiac Disease From the Working Group on Prolamin Analysis and Toxicity

et al. 2020

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Celiac disease (CD) affects a growing number of individuals worldwide. To elucidate the causes for this increase, future multidisciplinary collaboration is key to understanding the interactions between immunoreactive components in gluten-containing cereals and the human gastrointestinal tract and immune system and to devise strategies for CD prevention and treatment beyond the gluten-free diet. During the last meetings, the Working Group on Prolamin Analysis and Toxicity (Prolamin Working Group, PWG) discussed recent progress in the field together with key stakeholders from celiac disease societies, academia, industry and regulatory bodies. Based on the current state of knowledge, this perspective from the PWG members provides recommendations regarding clinical, analytical and legal aspects of CD. The selected key topics that require future multidisciplinary collaborative efforts in the clinical field are to collect robust data on the increasing prevalence of CD, to evaluate what is special about gluten-specific T cells, to study their kinetics and transcriptomics and to put some attention to the identification of the environmental agents that facilitate the breaking of tolerance to gluten. In the field of gluten analysis, the key topics are the precise assessment of gluten immunoreactive components in wheat, rye and barley to understand how these are affected by genetic and environmental factors, the comparison of different methods for compliance monitoring of gluten-free products and the development of improved reference materials for gluten analysis.

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Gluten Free Wheat: Are We There?

Cited by 52 publications

References 62 publications

Gene editing in plants: assessing the variables through a simplified case study

Gene editing in plants: assessing the variables through a simplified case study

CRISPR/Cas9 Gene Editing of Gluten in Wheat to Reduce Gluten Content and Exposure—Reviewing Methods to Screen for Coeliac Safety

Recent Progress and Recommendations on Celiac Disease From the Working Group on Prolamin Analysis and Toxicity

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