Glutathionylation of the Aquaporin-2 Water Channel

Tamma, Grazia; Ranieri, Marianna; Mise, Annarita Di; Centrone, Mariangela; Svelto, María; Valenti, Giovanna

doi:10.1074/jbc.m114.586024

Cited by 34 publications

(17 citation statements)

References 25 publications

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“…However, preliminary in silico analysis using NetOGlyc [ 33 ] and NetPhos [ 34 ] revealed the presence of several putative glycosylation and phosphorylation sites on RmAQP2 (data not shown). Post-translational modifications of AQP have been previously described [ 35 – 37 ] and it may be one explanation for the detection of larger protein products in the immunoblot assays. Structural predictions suggest that this protein may exist in the membrane as a tetramer and the multiple banding pattern in the blot could represent varying stages of the breakdown of this tetrameric structure; cleavage of the intracellular tails of the RmAQP2 would result in a molecular mass of ≈ 25 KDa, suggesting that the larger 50 KDa band could be a dimer of two molecules.…”

Section: Discussionmentioning

confidence: 99%

Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness

et al. 2015

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BackgroundTicks are blood-feeding arthropods that can affect human and animal health both directly by blood-feeding and indirectly by transmitting pathogens. The cattle tick Rhipicephalus (Boophilus) microplus is one of the most economically important ectoparasites of bovines worldwide and it is responsible for the transmission of the protozoan Babesia bovis, the etiological agent of bovine babesiosis. Aquaporins (AQPs) are water channel proteins implicated in physiological mechanisms of osmoregulation. Members of the AQP family are critical for blood-feeding arthropods considering the extreme osmoregulatory changes that occur during their feeding. We investigated the pattern of expression of a newly identified AQP2 gene of R. microplus (RmAQP2) in different tick tissues and stages. We also examined in vivo the biological implications of silencing expression of RmAQP2 silencing during tick feeding on either uninfected or B. bovis-infected cattle.MethodsIn silico gene analyses were performed by multiple alignments of amino acid sequences and topology prediction. Levels of RmAQP2 transcripts in different tick tissues and stages were analyzed by reverse transcriptase quantitative PCR. Patterns of expression of RmAQP2 protein were investigated by immunoblots. Gene silencing was performed by RNA interference and in vivo functional analyses carried out by feeding ticks on either uninfected or B. bovis-infected cattle.ResultsRmAQP2 transcripts were found in unfed larvae, engorged nymphs, and salivary glands and guts of partially engorged females; however, of all tick tissues and stages examined, RmAQP2 protein was found only in salivary glands of partially engorged females. RmAQP2 silencing significantly reduced tick fitness and completely abrogated protein expression. The effect of RmAQP2 silencing on fitness was more pronounced in females fed on a B. bovis-infected calf than in ticks fed on an uninfected calf and none of their larval progeny survived.ConclusionsCollectively, considering the gene expression and tick fitness data, we conclude that RmAQP2 is critical for tick blood feeding and may be a suitable candidate target for the development of novel strategies to control R. microplus and tick-borne parasites.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-1226-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness

et al. 2015

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“…It was also found that the increase in AQP2 glutathionylation is paralleled by higher ROS production. Conversely, low levels of ROS, measured in cells displaying low intracellular calcium concentration, secondary to the expression of the calcium-sensing receptor (CaSR), associates with reduced S-glutathionylation of AQP2 [ 130 ]. Whether or not S-glutathionylation of AQP2 is involved in the water imbalance observed during aging remains to be investigated.…”

Section: Role Of Vasopressin/aqp2 Axis and Oxidative Stress In Agimentioning

confidence: 99%

Aquaporin Membrane Channels in Oxidative Stress, Cell Signaling, and Aging: Recent Advances and Research Trends

Tamma

Valenti

Grossini

et al. 2018

Oxidative Medicine and Cellular Longevity

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Reactive oxygen species (ROS) are produced as a result of aerobic metabolism and as by-products through numerous physiological and biochemical processes. While ROS-dependent modifications are fundamental in transducing intracellular signals controlling pleiotropic functions, imbalanced ROS can cause oxidative damage, eventually leading to many chronic diseases. Moreover, increased ROS and reduced nitric oxide (NO) bioavailability are main key factors in dysfunctions underlying aging, frailty, hypertension, and atherosclerosis. Extensive investigation aims to elucidate the beneficial effects of ROS and NO, providing novel insights into the current medical treatment of oxidative stress-related diseases of high epidemiological impact. This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. The most recent advances regarding the modulation exerted by food phytocompounds with antioxidant action on AQPs are also reviewed.

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“…This effect was blunted by phosphodiesterases inhibitors, suggesting that O 2 − enhances cAMP degradation [ 99 ]. In addition, exposure of mice kidney slices to tert-butyl hydroperoxide increases AQP2 glutathionylation [ 100 ]. Taken together these data indicate that O 2 − and H 2 O 2 participate in the regulation of AQP2, but fundamental measurements of water permeability are missing.…”

Section: Collecting Ductsmentioning

confidence: 99%

Effects of Reactive Oxygen Species on Tubular Transport along the Nephron

Gonzalez‐Vicente

Garvin

2017

Antioxidants

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Reactive oxygen species (ROS) are oxygen-containing molecules naturally occurring in both inorganic and biological chemical systems. Due to their high reactivity and potentially damaging effects to biomolecules, cells express a battery of enzymes to rapidly metabolize them to innocuous intermediaries. Initially, ROS were considered by biologists as dangerous byproducts of respiration capable of causing oxidative stress, a condition in which overproduction of ROS leads to a reduction in protective molecules and enzymes and consequent damage to lipids, proteins, and DNA. In fact, ROS are used by immune systems to kill virus and bacteria, causing inflammation and local tissue damage. Today, we know that the functions of ROS are not so limited, and that they also act as signaling molecules mediating processes as diverse as gene expression, mechanosensation, and epithelial transport. In the kidney, ROS such as nitric oxide (NO), superoxide (O2−), and their derivative molecules hydrogen peroxide (H2O2) and peroxynitrite (ONO2−) regulate solute and water reabsorption, which is vital to maintain electrolyte homeostasis and extracellular fluid volume. This article reviews the effects of NO, O2−, ONO2−, and H2O2 on water and electrolyte reabsorption in proximal tubules, thick ascending limbs, and collecting ducts, and the effects of NO and O2− in the macula densa on tubuloglomerular feedback.

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Glutathionylation of the Aquaporin-2 Water Channel

Cited by 34 publications

References 25 publications

Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness

Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness

Aquaporin Membrane Channels in Oxidative Stress, Cell Signaling, and Aging: Recent Advances and Research Trends

Effects of Reactive Oxygen Species on Tubular Transport along the Nephron

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