1996
DOI: 10.1006/pest.1996.0026
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GlutathioneS-Transferase Activity in Unsafened and Fenclorim-Safened Rice (Oryza sativa)

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Cited by 38 publications
(33 citation statements)
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“…However, the highest GST activities measured in the wheat varieties BRS 49 and CD 104 did not reveal more tolerance to fenoxaprop-p-ethyl, because visual evaluations indicated that these varieties had higher susceptibility to this herbicide (Table 1). These results are contradictory to those of Wu et al, (1996) who considered that an increase in GST activity in wheat is one of the mechanisms responsible for fenoxaprop-pethyl detoxification, improving tolerance to the herbicide. Furthermore, field populations of the black-grass weed (Alopecurus myosuroides Huds.…”
Section: Resultscontrasting
confidence: 55%
See 1 more Smart Citation
“…However, the highest GST activities measured in the wheat varieties BRS 49 and CD 104 did not reveal more tolerance to fenoxaprop-p-ethyl, because visual evaluations indicated that these varieties had higher susceptibility to this herbicide (Table 1). These results are contradictory to those of Wu et al, (1996) who considered that an increase in GST activity in wheat is one of the mechanisms responsible for fenoxaprop-pethyl detoxification, improving tolerance to the herbicide. Furthermore, field populations of the black-grass weed (Alopecurus myosuroides Huds.…”
Section: Resultscontrasting
confidence: 55%
“…o C, the supernatant was collected and used as crude extract to assess GST activity according to Wu et al (1996) using 1 chloro-2,4-dinitrobenzene (CDNB) as substrate. Each reaction (3 mL) contained 30 µL enzyme extract, 2 mL 100 mM potassium phosphate buffer pH 6.9, 0.9 mL 3.3 mM GSH (0.9 mL), and 100 µL 30 mM CDNB in 96% ethanol.…”
Section: Gst Extraction and Assaymentioning
confidence: 99%
“…As amostras para determinações enzimáticas foram obtidas a partir de extratos da matéria fresca da parte aérea das plantas nas duas épocas de coleta. Estas determinações seguiram o método proposto por Wu (1996) para GST e o proposto por Giannopolitis & Ries (1977) para SOD. O conteúdo de proteína solúvel total nos extratos enzimáticos foi determinado de acordo com o método de Lowry et al (1951).…”
Section: Methodsunclassified
“…A atividade da GST foi analisada de acordo com a metodologia descrita por Wu et al (1996). A mistura de reação foi composta de 30 µL de extrato enzimático, 2.000 µL de tampão fosfato de potássio pH 6,9 100 mmol L 1 , 900 µL de glutationa reduzida (GSH) 3,3 mmol L -1 e 100 µL de 1-cloro-2,4-dinitrobenzeno (CDNB) 30 mmol L -1 .…”
Section: Methodsunclassified