Glutathione triggered degradation of polydopamine to facilitate controlled drug release for synergic combinational cancer treatment

Hao, Yanan; Zheng, Anqi; Guo, Tingting; Shu, Yang; Wang, Jianhua; Johnson, Omar; Chen, Wei

doi:10.1039/c9tb01400d

Cited by 52 publications

(36 citation statements)

References 57 publications

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“…[ 34,40 ] Glutathione has also been shown to degrade polydopamine and is present in different cell types. [ 41 ] Degradability of polydopamine has been explicitly demonstrate in in vivo experiments as well. [ 39,42 ] In particular, intravenous injection of polydopamine was shown before to have an excellent safety profile.…”

Section: Introductionmentioning

confidence: 99%

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Harizaj

Wels

Raes

et al. 2021

Adv Funct Materials

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Safe and efficient production of chimeric antigen receptor (CAR)‐T cells is of crucial importance for cell‐based cancer immunotherapy. Physical transfection methods have quickly gained in importance in the context of transfecting T‐cells, since they are readily compatible with different cell types and a broad variety of cargo molecules. In particular, nanoparticle‐sensitized photoporation has been introduced in recent years as a gentle yet efficient method to transiently permeabilize cells, allowing subsequent entry of external cargo molecules into the cells. Gold nanoparticles (AuNPs) have been used the most as photothermal sensitizers because they can easily form laser‐induced vapor nanobubbles, a photothermal phenomenon that is shown to be particularly efficient for permeabilizing cells. However, as AuNPs are not biodegradable, clinical translation is hampered. Here, for the first time, the possibility to form laser‐induced vapor nanobubbles from biocompatible polymeric nanoparticles is reported. Compared to electroporation, the most used physical transfection method for T cells, 2.5 times more living mRNA transfected human T cells are obtained via photoporation sensitized by polydopamine nanoparticles. This shows that photoporation is a viable approach for efficiently producing therapeutic engineered T‐cells at a throughput easily exceeding 105 cells per second.

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Section: Introductionmentioning

confidence: 99%

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Harizaj

Wels

Raes

et al. 2021

Adv Funct Materials

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“…The degradation of melanin with a PNs-like structure has been shown to take more than 8 weeks in vivo 23 . It has been reported that PDA is degraded in the cytoplasm via the reducing catalytic activity of glutathione 25 . It has been shown that intravenous injection of PNs does not cause histological or hematological toxicity in mice 6 .…”

Section: Discussionmentioning

confidence: 99%

Molecular engineering of antibodies for site-specific conjugation to lipid polydopamine hybrid nanoparticles

Yang

Shim

et al. 2020

Acta Pharmaceutica Sinica B

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Conjugation of antibodies to nanoparticles allows specific cancer targeting, but conventional conjugation methods generate heterogeneous conjugations that cannot guarantee the optimal orientation and functionality of the conjugated antibody. Here, a molecular engineering technique was used for site-specific conjugation of antibodies to nanoparticles. We designed an anti-claudin 3 (CLDN3) antibody containing a single cysteine residue, h4G3cys, then linked it to the maleimide group of lipid polydopamine hybrid nanoparticles (LPNs). Because of their negatively charged lipid coating, LPNs showed high colloidal stability and provided a functional surface for site-specific conjugation of h4G3cys. The activity of h4G3cys was tested by measuring the binding of h4G3cys-conjugated LPNs (C-LPNs) to CLDN3-positive tumor cells and assessing its subsequent photothermal effects. C-LPNsspecifically recognized CLDN3-overexpressing T47D breast cancer cells but not CLDN3-negative Hs578T breast cancer cells. High binding of C-LPNs to CLDN3-overexpressing T47D cells resulted in significantly higher temperature generation upon NIR irradiation and potent anticancer photothermal efficacy. Consistent with this, intravenous injection of C-LPNsin a T47D xenograft mouse model followed by NIR irradiation caused remarkable tumor ablation compared with other treatments through high temperature increases. Our results establish an accurate antibody-linking method and demonstrate the possibility of developing therapeutics using antibody-guided nanoparticles.

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“…The morphologies and structures of the PEG modified exosomes were confirmed by TEM, cryo‐TEM, zeta‐potential, FTIR, and SEC. As it is possible for PDA to become permeable and disassemble in physiologically relevant environments, [14,21,22] the successful engineering of PDA and PEG‐modified exosomes provides promise for future applications.…”

Section: Figurementioning

confidence: 99%

Polydopamine‐Mediated Surface Functionalization of Exosomes

Wang

Kimura

et al. 2021

ChemNanoMat

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Exosomes are cell‐derived extracellular vesicles that hold promise for diagnosis and therapy. The surface characteristics of exosomes dictate their biological fate and are therefore important for various applications, however surface modification strategies are generally either limited in scope or require complex equipment or experimental procedures. Herein, we employ a simple and fast method to control the surface properties of exosomes via polydopamine coating. Specifically, the polydopamine coating allowed for tailorable functionalization via secondary reactions, which opens exciting avenues for the application of exosomes.

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Glutathione triggered degradation of polydopamine to facilitate controlled drug release for synergic combinational cancer treatment

Abstract: Here we report a novel mechanism for triggering drug release in the polydopamine (PDA)-coated magnetic CuCo2S4 core–shell nanostructure by glutathione (GSH) triggered degradation of PDA for release.

Cited by 52 publications

References 57 publications

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Molecular engineering of antibodies for site-specific conjugation to lipid polydopamine hybrid nanoparticles

Polydopamine‐Mediated Surface Functionalization of Exosomes

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