Glutathione synthesis during in vitro maturation of buffalo (Bubalus bubalis) oocytes: effects of cysteamine on embryo development

Gasparrini, B.; Sayoud, Hichem; Neglia, Gianluca; Matos, D.G. de; Donnay, Isabelle; Zicarelli, L.

doi:10.1016/s0093-691x(03)00098-0

Cited by 68 publications

(52 citation statements)

References 32 publications

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“…A positive effect of GSH concentration on oocyte developmental competence has been demonstrated, and therefore the intracellular GSH level is used as a marker for oocyte cytoplasmic maturation (de Matos et al 1995, Abeydeera et al 1998, Gasparrini et al 2003. Hyperthermia-induced oxidative stress has been suggested as one of the mechanisms by which heat stress disrupts reproductive performance.…”

Section: Discussionmentioning

confidence: 99%

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Wang¹,

Sui²,

Miao³

et al. 2009

Reproduction

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The objectives of this study were to investigate the effect of heat stress during in vitro maturation on the developmental potential of mouse oocytes and to determine whether the deleterious effect was on the nuclear or cytoplasmic component. While rates of oocyte nuclear maturation (development to the metaphase II stage) did not differ from 37 to 40 8C, rates for blastocyst formation decreased significantly as maturation temperature increased from 38.5 to 39 8C. Chromosome spindle exchange showed that while blastocyst formation did not differ when spindles matured in vivo or in vitro at 37, 40 or 40.7 8C were transplanted into in vivo matured cytoplasts, no blastocyst formation was observed when in vivo spindles were transferred into the 40 8C cytoplasts. While oocytes reconstructed between 37 8C ooplasts and 37 or 40 8C karyoplasts developed into 4-cell embryos at a similar rate, no oocytes reconstituted between 40 8C ooplasts and 37 8C spindles developed to the 4-cell stage. Immunofluorescence microscopy revealed impaired migration of cortical granules and mitochondria in oocytes matured at 40 8C compared with oocytes matured at 37 8C. A decreased glutathione/GSSG ratio was also observed in oocytes matured at 40 8C. While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 8C, spindle assembling was affected and MAD2 was activated in some of the oocytes matured at 40.7 8C. It is concluded that 1) oocyte cytoplasmic maturation is more susceptible to heat stress than nuclear maturation, and 2) cytoplasmic rather than nuclear components determine the pre-implantation developmental capacity of an oocyte.

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Section: Discussionmentioning

confidence: 99%

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Wang¹,

Sui²,

Miao³

et al. 2009

Reproduction

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show abstract

“…Konsentrasi kadar lemak yang tinggi pada oosit dan media kultur embrio kerbau secara in vitro, sangat berpengaruh dan sensitif terhadap peningkatan stres oksidatif yang terjadi dalam kondisi in vitro (Gasparrini et al, 2003).…”

Section: Pendahuluanunclassified

“…De Matos et al, 2002;Gasparrini et al, 2003;Beheshti et al, 2011, bahwa media kultur embrio in vitro yang mengandung prekursor GSH seperti cysteamine dan β-mercaptoethanol akan meningkatkan kandungan GSH dan memiliki efek yang menguntungkan pada perkembangan embrio 8 sel sampai tahap blastosit. Menurut yang dinyatakan oleh de Matos et al, 2002dan Songsasen et al, 2002, bahwa suplementasi senyawa tiol akan meningkatkan sistesis GSH dalam media kultur dan meningkatkan kualitas embrio kerbau yang di kultur semakin baik.…”

Section: Persentaseunclassified

“…Menurut yang dinyatakan oleh de Matos et al, 2002dan Songsasen et al, 2002, bahwa suplementasi senyawa tiol akan meningkatkan sistesis GSH dalam media kultur dan meningkatkan kualitas embrio kerbau yang di kultur semakin baik. Menurut Gasparrini et al, 2003, oosit kerbau yang dikultur secara in vitro dapat mensintesis GSH secara kimiawi. GSH adalah nonprotein sebuah senyawa sulphydryl dan memainkan peran penting dalam melindungi sel mamalia dari stres oksidatif dan sintesis intraseluler yang sangat penting dalam kultur embrio (Gordon, 2003;Gasparrini et al, 2003;Ruder, 2008).…”

Section: Persentaseunclassified

“…Menurut Gasparrini et al, 2003, oosit kerbau yang dikultur secara in vitro dapat mensintesis GSH secara kimiawi. GSH adalah nonprotein sebuah senyawa sulphydryl dan memainkan peran penting dalam melindungi sel mamalia dari stres oksidatif dan sintesis intraseluler yang sangat penting dalam kultur embrio (Gordon, 2003;Gasparrini et al, 2003;Ruder, 2008). Kim et al, (2004) melaporkan glutathione (GSH) memiliki banyak fungsi seperti dalam mempertahankan reaksi redoks dalam sel yang dikultur, meningkatkan pembentukan pronukleus jantan, protein dan sintesis DNA, dan pengurangan disulfifi des.…”

Section: Persentaseunclassified

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Fertilitas Dan Persentase Embrio Kerbau Sampai Morula Yang Dikultur Dengan Penambahan Glutathione Secara in Vitro

Harissatria

Hendri

2016

jupet

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Developmental capability of denuded bovine oocyte in a Co‐culture system with intact cumulus‐oocyte complexes: Role of cumulus cells, cyclic adenosine 3′,5′‐monophosphate, and glutathione

Luciano

Lodde

Beretta

et al. 2005

Molecular Reproduction Devel

147

116

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Cumulus oophorus cells have been implicated in the regulation of female gamete development, meiotic maturation, and oocyte-sperm interaction. Nevertheless, the specific role of cumulus cells (CCs) during the final stages of oocyte maturation and fertilization processes still remains unclear. Several studies have been conducted in order to clarify the role of follicular cells using culture systems where denuded oocytes (DOs) were co-cultured with isolated CCs, or in the presence of conditioned medium. However, those attempts were ineffective and the initial oocyte competence to become a blastocyst after fertilization was only partially restored. Aim of the present study was to analyze the effect of the interactions between somatic cells and the female gamete on denuded oocyte developmental capability using a system of culture where CCs were present as dispersed CCs or as intact cumulus-oocyte complexes (COCs) in co-culture with oocytes freed of CC investment immediately after isolation from the ovary. Moreover, we analyzed the specific role of cyclic adenosine 3'-5' monophosphate (cAMP) and glutathione (GSH) during FSH-stimulated maturation of denuded oocyte co-cultured with intact COCs. Our data confirm that denuded oocyte has a scarce developmental capability, and the presence of dispersed CCs during in vitro maturation (IVM) does not improve their developmental competence. On the contrary, the co-presence of intact COCs during denuded oocyte IVM partially restores their developmental capability. The absence of CCs investment causes a drop of cAMP content in DOs at the beginning of IVM and the addition of a cAMP analog in the culture medium does not restore the initial oocyte developmental competence. The relative GSH content of denuded oocyte matured in presence of intact COCs is consistent with the partial recovery of their developmental capability. However, the complete restoration of a full embryonic developmental potential is achieved only when DOs are co-cultured with intact COCs during both IVM and in vitro fertilization (IVF). Our results suggest that the direct interaction between oocyte and CCs is not essential during IVM and IVF of denuded oocyte. We hypothesize that putative diffusible factor(s), produced by CCs and/or by the crosstalk between oocyte and CCs in the intact complex, could play a key role in the acquisition of developmental competence of the denuded female gamete.

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Glutathione synthesis during in vitro maturation of buffalo (Bubalus bubalis) oocytes: effects of cysteamine on embryo development

Cited by 68 publications

References 32 publications

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Fertilitas Dan Persentase Embrio Kerbau Sampai Morula Yang Dikultur Dengan Penambahan Glutathione Secara in Vitro

Developmental capability of denuded bovine oocyte in a Co‐culture system with intact cumulus‐oocyte complexes: Role of cumulus cells, cyclic adenosine 3′,5′‐monophosphate, and glutathione

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