Glutathione S-transferase T1 and myeloperoxidase −463 G>A genotypes in lung cancer patients of Kumaun region

Abstract

Background:Null genetic polymorphism of Glutathione S-transferase T1 (GSTT1) and -463 G>A promoter polymorphism of myeloperoxidase (MPO) were studied for association with lung cancer.Materials and Methods:In a case- control study 26 lung cancer patients and 33 healthy individuals from hilly Kumaun region of northern India were investigated. DNA was extracted from peripheral blood. GSTT1 null polymorphism was detected by duplex PCR, and MPO polymorphism was detected by performing PCR-RFLP.Results:An increased but statistically non- significant risk for lung cancer was found for GSTT1 null genotype. No association for MPO -463G>A genotype was evident.Conclusion:Further study with large sample size may reveal such association in this population.

Association of 12 polymorphic variants conferring genetic risk to lung cancer in Indian population: An extensive meta‐analysis

“…Several genome-wide association studies (GWAS) have so far identified 16 susceptibility loci (P 5.00 3 10 -8 ) worldwide that are associated with lung cancer risk [Wang et al, 2008;Hu et al, 2011;Dong et al, 2012] and four loci out of them showed evidences of association of lung cancer risk in smokers [Dong et al, 2012]. However, most of these reports were based on Caucasian and Chinese populations, and many identified risk alleles have not been adequately evaluated in Indian population in spite of several case-control investigations on the Indian population from different geographical zones [Sobti et al, 2003[Sobti et al, , 2004[Sobti et al, , 2008[Sobti et al, , 2009Sreeja et al, 2005Sreeja et al, , 2008aSreeja et al, , 2008bSreeja et al, , 2008cJain et al, 2005;Pachouri et al, 2006Pachouri et al, , 2007Kotnis et al, 2008;Shah et al, 2008;Singh et al, 2010Singh et al, , 2016Tilak et al, 2011Tilak et al, , 2013Ihsan et al, 2011a,;Kant Shukla et al, 2013;Natukula et al, 2013;Shukla et al, 2013;Bag et al, 2014;Phukan et al, 2014;Saikia et al, 2014;Uppal et al, 2014;Sharma et al, 2015;Kumari et al, 2016;Peddireddy et al, 2016]. To date, researchers have not been able to delineate the overall effect of the genes and the genetic variations on lung cancer susceptibility in the populations of Indian origin, due to certain conflicting results.…”

Genetic Variants Modifying the Risk of Lung Cancer and Its Subtypes: A Comprehensive Meta-analysis and a Case-control Study

“…The age and pack-years of smoking were converted to categorical variables based on their respective mean values. The case-control analysis revealed no signi cant association of rs1048943 of CYP1A1 in additive effect model (GG vs GA vs. AA OR=1 24,. 95% CI= 0.79-1.93; p adj =0.35), dominant effect model (GG+GA vs. AA: OR=1.33, 95% CI= 0.825-2.16; P adj =0.24), and recessive effect model (GG vs AG+AA: OR=0.49, 95% CI=0.1-4.56; p adj =0.53) with lung cancer adjusted for pack-years of smoking, age, alcohol consumption, tobacco and betel quid chewing, and asbestos exposure (Supplementary Information,…”

“…Out of the 10 loci, four were associated with overall lung cancer risk, while the remaining 6 loci were found to be associated with lung adenocarcinoma 21 . Most of the GWAS was done on subjects of European or Chinese descent, and the majority of the identi ed risk alleles have not been evaluated in the population of the Indian subcontinent despite several candidate gene association studies [24][25][26][27][28][29][30][31][32][33] . Contradictory outcomes of case-control association studies of the same polymorphism by different authors failed to identify the overall effect of the genes and the genetic variations on lung cancer susceptibility in the region.…”

Genetic Polymorphisms Associated with Environmental Exposure to Polycyclic Derivatives in African Children

“…DNA extraction was performed in peripheral blood leukocytes using DNA Kit 250 FlexiGene Qiagen (Hilden, North Rhine-Westphalia, Germany) in the Laboratório de Investigação em Genética e Hematologia Translacional (LIGHT) of the IGM-FIOCRUZ. Evaluation of CYP2E1 -1293G>C/-1053C>T (rs3813867/rs2031920), NQO1 609C>T (rs1800566), and MPO -463G>A (rs2333227) gene polymorphisms were performed by PCR/RFLP (polymerase chain reaction/restriction fragment length polymorphism) [ 10 , 11 , 19 ]. Analysis of GSTT1 / GSTM1 gene polymorphisms was performed by multiplex PCR, using the HBB (beta-globin) gene as an endogenous marker for the reaction [ 14 ].…”

“…This enzyme is abundant in neutrophils and monocytes and catalyzes hydrogen peroxide (H 2 O 2 ) to hypochlorous acid (HOCl), a potent oxidant. The polymorphism MPO -463G>A leads to enzymatic changes, affecting the metabolism of xenobiotics, which is related to the formation of DNA adducts [ 3 , 11 – 13 ].…”