Glutathione S-transferase T1-1 activity upregulated in transitional cell carcinoma of urinary bladder

Simić, Tatjana; Mimic-Oka, Jasmina; Savić-Radojević, Ana; Opačić, Miloš; Pljesa, M.; Dragičević, Dejan; Djokic, M.; Radosavljevic, R.

doi:10.1016/j.urology.2005.01.005

Cited by 32 publications

(33 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tumor GSTT1 mRNA expression was correlated with tumor stage and grade, and showed a tendency toward a positive correlation with tumor aggressiveness. These findings are in accordance with those of Simic et al [24]. Although many studies have investigated polymorphisms in GSTM1 and GSTT1, findings between ethnic groups have been inconsistent [12][13][14].…”

Section: Discussionsupporting

confidence: 93%

GSTT1: A Marker of the Aggressiveness of Bladder Cancer

Yan

Park

et al. 2010

Urol Int

View full text Add to dashboard Cite

Introduction: Glutathione S-transferases have been implicated in the development of bladder cancer (BC). We investigated the genotype and expression of glutathione S-transferase-mu (GSTM1) and glutathione S-transferase-theta (GSTT1) in BC tissue specimens. Materials and Methods: Tumor samples and matched normal mucosae were obtained from 34 patients. Genomic DNA was used to analyze GSTM1 and GSTT1 genotypes using multiplex polymerase chain reaction. GSTM1 and GSTT1 mRNA levels were measured using real-time reverse transcriptase polymerase chain reaction. Results:GSTM1 mRNA expression was lower in tumor tissues than in matched normal bladder mucosae, whereas GSTT1 mRNA expression was significantly higher. GSTT1 mRNA expression was higher in muscle-invasive BC and high-grade cancers than in non-muscle-invasive BC and lower-grade tumors. Conclusions:GSTT1 is correlated with characteristics of aggressive BC. GSTT1 may play an important role in tumorigenesis and disease progression in patients with BC.

show abstract

Section: Discussionsupporting

confidence: 93%

GSTT1: A Marker of the Aggressiveness of Bladder Cancer

Yan

Park

et al. 2010

Urol Int

View full text Add to dashboard Cite

show abstract

“…Moreover, changes in GSTs have been associated with tumor stage, aggressiveness and cancer progression [9][10][11][12] and also resistance to chemotherapy [20]. GST activity was also found to be higher in the blood of bladder cancer individuals than in controls [21] and high plasma GSTA1 and GSTP1 levels were more frequently observed in urinary bladder cancer patients than in controls [22].…”

Section: Discussionmentioning

confidence: 99%

“…GSTs protein expression and activity was extensively investigated, while SOD2 and NRF2 transcript levels have been seldom studied in urinary bladder cancer. Several studies report that urinary bladder tumors have been shown to express high levels of various GST isoenzymes compared with surrounding non-malignant uroephitelium [9][10][11][12]. Similarly, SOD2 and NRF2 was up-regulated in human bladder cancers and tumor cells [13,14].…”

mentioning

confidence: 99%

Expression of NRF2 and NRF2-modulated genes in peripheral blood leukocytes of bladder cancer males

Reszka

Jabłonowski²,

Wieczorek³

et al. 2012

neo

View full text Add to dashboard Cite

Nuclear factor (erythroid-derived 2)-like 2 (NRF2) is an oxidant-responsive transcription factor involved in induction of antioxidant genes. We assessed NRF2 and selected NRF2-modulated gene expression: glutathione S-transferase A1 and P1 (GSTA1 and GSTP1), mitochondrial superoxide dismutase (SOD2) in blood leukocytes of 51 bladder cancer patients and 90 control males. A significant up-regulation of SOD2 expression (P=0.002) was observed in leukocytes of patients. NRF2 expression was positively correlated with GSTP1 and with SOD2 mRNA level, both in patients and controls.These data suggest disturbances in SOD2 transcription in circulating blood leukocytes of males with bladder cancer. Moreover, concomitant constitutive expression of NRF2 and its target genes may suggest important role of NRF2 transcription factor in positive regulation of antioxidant genes, resulted in enhanced cytoprotection in human peripheral blood leukocytes.

show abstract

“…After thawing, the cytosolic fraction was prepared for the determination of enzyme activity and expression, as described elsewhere [12]. For the determination of glutathione content , the frozen tissues were homogenized in 5 ′ -sulfosalicylic acid (10%, w/v), centrifuged for 20 min at 3000g (4°C), and the supernatant fraction was used.…”

Section: Patients Tissue Specimens and Sample Preparationmentioning

confidence: 99%