Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) variants and breast cancer risk in Burkina Faso

Abstract

Background and objectiveBreast cancer remains the most common cause of cancer mortality in women. The aim of this study was to investigate associations between genetic variability in GSTM1 and GSTT1 and susceptibility to breast cancer.MethodsGenomic DNA was extracted from blood samples for 80 cases of histologically diagnosed breast cancer and 100 control subjects. Genotyping analyses were performed by PCR-based methods. Associations between specific genotypes and the development of breast cancer were examined using logistic regression to calculate odds ratios [1] and 95% confidence intervals (95%CI).ResultsNo correlation was found between GSTM1-null and breast cancer (OR = 1.83; 95%CI 0.90-3.71; p = 0.10), while GSTT1-null (OR = 2.42; 95%CI 1.17-5.02; p= 0.01) was associated with increased breast cancer risk. The GSTM1/GSTT1 double null was not associated with an increased risk of developing breast cancer (OR = 2.52; 95%CI 0.75-8.45; p = 0.20). Furthermore, analysis found no association between GSTM1-null (OR =1.12; 95%CI 0.08-15.50; p = 1.00) or GSTT1-null (OR = 1.71; 95%CI 0.13-22.51; p = 1.00) and the disease stage of familial breast cancer patients or sporadic breast cancer patients (GSTM1 (OR = 0.40; 95%CI 0.12-1.32; p = 0.20) and GSTT1 (OR = 1.41; 95%CI 0.39-5.12; p = 0.75)). Also, body mass index (BMI) was not associated with increased or decreased breast cancer risk in either GSTM1-null (OR = 0.60; 95%CI 0.21-1.68; p = 0.44) or GSTT1-null (OR = 0.60; 95%CI 0.21-1.68; p =0.45).ConclusionOur results suggest that only GSTT1-null is associated with increased susceptibility to breast cancer development.

Genetic Susceptibility to Breast Cancer in Sub-Saharan African Populations

“…There were no GWASs among the 22 papers. The studies in this review included populations from 10 of the 46 SSA countries: Burkina Faso, 22 , 27 , 37 Cameroon, 21 Democratic Republic of Congo, 25 Ethiopia, 38 Kenya, 19 Nigeria, 23 , 28 , 30 , 32 , 33 , 35 , 36 Rwanda, 29 , 39 , 40 Senegal, 20 , 26 , 34 South Africa, 24 , 31 and Uganda. 21 …”

“… 35 A study of the glutathione S-transferase null variants in the GSTM1 and GSTT1 genes in cases and controls from Burkina Faso found no association of the GSTM1 null variant with BC, whereas the GSTT1 null allele was associated with an increase in BC risk (OR = 2.42; 95% CI, 1.17 to 5.02; P = .01). 37 Investigation of an initiation codon variant (Met1Arg, rs2228570) in the vitamin D receptor ( VDR ) gene in 392 Ethiopian BC cases and controls reported an association of the Arg/Arg (G/G) genotype with an increased risk of BC (OR = 1.44; 95% CI, 1.01 to 2.06; P = .04). 38 A lack of association of a functional SNP in the GPX1 gene (p.Pro198Leu) and the TP53 functional SNP (p.Pro72Arg) with BC was reported in the Rwandese population, 39 , 40 but the sample sizes were very small.…”

Glutathione S-transferase M1 and T1 genes deletion polymorphisms and blood pressure control among treated essential hypertensive patients in Burkina Faso

“…The frequencies of GSTM null and GSTT1 null varies widely in different populations. Previous studies in Burkina Faso reported that approximately 28.75%–31.23% and 30%–55.76% of the population have the null variant of GSTM1 and GSTT1 respectively [ 12 , 38 ], which fell into the range of allele frequency values registered in west African Nigeria (0.3 for GSTM1 null allele; 0.37 for GSTT1 null allele) [ 39 ] and other Black African populations [ 40 ]. GSTM1 and GSTT1 play a key role in the metabolism of certain drugs and xenobiotic through their participation in the second phase of xenobiotic metabolism.…”

Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region

“…Двойная делеция GSTM1 / GSTT1 не увеличивала риск развития РМЖ (ОШ 2,52; 95 % ДИ 0,75-8,45; p = 0,20). Кроме того, проведенный анализ не обнаружил связи между «нулевыми» генотипами и стадией заболевания, а также наличием семейного или спорадического рака [4].…”

“…Одними из наиболее распространенных полиморфизмов являются делеции генов GSTM1 и GSTT1, которые приводят к отсутствию соответствующих ферментов. В многочисленных исследованиях показано, что делеционный полиморфизм GSTM1 и GSTT1 может служить фактором предрасположенности к развитию различных заболеваний, таких как РМЖ [4], рак шейки матки [5], рак легкого [6], рак мочевого пузыря [7], хроническая обструктивная болезнь легких [8] и др., а также оказывать влияние на эффективность действия химиотерапевтических препаратов [9].…”