Glutathione S-transferase class ? in French alcoholic cirrhotic patients

Groppi, Alexis; Coutelle, C.; Fleury, Benoît; Iron, Albert; Bégueret, Joël; Couzigou, Patrice

doi:10.1007/bf00209028

Cited by 55 publications

(25 citation statements)

References 19 publications

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“…Detailed information of the PCR assays can be found elsewhere [Groppi et al, 1991]. Genotypes of GSTM1 were determined by the presence or absence of PCR product, according to the method of Groppi et al [1991]. Two primers, 5Ј-GAAGGTGGCCTC-CTCCTTGG-3Ј and 5Ј-AATTCTGGATTGTAGCAGAT-3Ј, were used for PCR.…”

Section: Polymorphisms Of Cyp1a1 and Gstm1mentioning

confidence: 99%

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Gender difference in DNA adduct levels among nonsmoking lung cancer patients

Cheng¹,

Hsieh²,

Lin³

et al. 2001

Environ and Mol Mutagen

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Lung cancer is the leading cause of cancer mortality in Taiwanese women. Cigarette smoking cannot explain the high lung cancer mortality in this population because less than 10% of women in Taiwan are smokers. Therefore, environmental factors other than smoking may play an important role in lung cancer development in female nonsmokers. The purpose of this study was to elucidate the role of environmental carcinogen exposure in lung cancer development in Taiwanese female nonsmokers, based on DNA adduct formation. We collected nontumorous lung tissues resected from 62 nonsmoking lung cancer patients and 20 noncancer controls to investigate whether differences in susceptibility to DNA adduct formation exist between men and women. (32)P-postlabeling and ELISA (enzyme-linked immunosorbent assay) with polyclonal antibody against BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene)-DNA adduct were used to evaluate DNA adduct levels in lung tissues of study subjects. Our data showed that the DNA adduct levels of lung cancer patients determined by both assays were significantly higher than those of noncancer controls (P = 0.0001 for (32)P-postlabeling; P = 0.01 for ELISA). Moreover, DNA adduct levels in females were markedly greater than those in males (P = 0.014 for (32)P-postlabeling; P = 0.001 for ELISA). The difference in DNA adduct levels could not be explained by genetic polymorphisms of cytochrome P-4501A1 (CYP1A1) or glutathione S-transferase (GSTM1), as determined by polymerase chain reaction and restriction fragment length polymorphism. These results demonstrate that lung cancer patients have a higher susceptibility to DNA damage than that of noncancer controls. In addition, differences in susceptibility to DNA damage derived from environmental carcinogen exposure were observed between male and female nonsmokers. In conclusion, high susceptibility to DNA damage in females may partially explain the high mortality rate of lung cancer in nonsmoking Taiwanese women.

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Section: Polymorphisms Of Cyp1a1 and Gstm1mentioning

confidence: 99%

“…The amplified products were digested with MspI and analyzed by electrophoresis on a 1.5% agarose gel. Detailed information of the PCR assays can be found elsewhere [Groppi et al, 1991]. Genotypes of GSTM1 were determined by the presence or absence of PCR product, according to the method of Groppi et al [1991].…”

Section: Polymorphisms Of Cyp1a1 and Gstm1mentioning

confidence: 99%

Gender difference in DNA adduct levels among nonsmoking lung cancer patients

Cheng¹,

Hsieh²,

Lin³

et al. 2001

Environ and Mol Mutagen

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“…Subjects were divided into three groups corresponding to homozygous wild (Ile/ Ile), heterozygous (Ile/Val) and homozygous mutant (Val/Val) types. The GSTM1 genetic polymorphism was determined by the method of Groppi et al (1991). The presence or absence of an ampli®cation product results in two subgroups corresponding to GSTM1 present or GSTM1 null.…”

Section: Genotypesmentioning

confidence: 99%

Aromatic DNA adducts in coke-oven workers, in relation to exposure, lifestyle and genetic polymorphism of metabolic enzymes

Zhang¹,

Ichiba²,

Feng

et al. 2000

International Archives of Occupational and Environmental Health

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“…GSTM1-A and GSTM1-B, and by one null allele, viz. GSTM1 0 (Groppi et al 1991;Harada et al 1992). The latter does not produce a protein product because of an expanded deletion (Seidegard et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of the GSTM1 0/0 genotype in French heavy smokers with various types of chronic bronchitis

et al. 1997

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A homozygous gene deletion of the glutathione S-transferase M1 (GSTM1) locus of genomic DNA from blood spots was studied by the polymerase chain reaction in a group of French heavy smokers (n = 361), which included patients with severe chronic bronchitis (SCB; n = 87), moderate chronic bronchitis (MCB: n = 102) and hard smokers (HS) with no permanent clinical symptoms of chronic bronchitis (n = 172). The GSTM1 0/0 genotype was found in 71.3% and 65.7% of cases in SCB and MCB, respectively, compared with only 47.1% in the control HS group (P = 0.0002). This latter figure (47.1%) is consistent with the average GSTM1 deletion frequency in French Caucasians. Moreover, the results showed a significant difference in the distribution of the GSTM1 0/0 genotype for both the SCB and MCB groups against the control HS group, according to gender (SCB: P = 0.001; MCB: P = 0.005), age (SCB: P = 0.0001; MCB: P = 0.005) and smoking history (SCB: P = 0.0001; MCB: P = 0.005). Thus, individuals homozygous for the GSTM1 gene deletion, especially in the under-41 age group (SCB: P = 0.001; MSB: P = 0.04) with an average smoking history of 16-30 pack-years (SCB: P = 0.002; MSB: P = 0.01) are more prone to chronic lung diseases, such as SCB and MCB, than are GSTM1 +/+ or 0/+ subjects. Population screening of young people for the identification of GSTM1 0/0 subjects, with special emphasis on smoking habits, might be useful (1) for the early detection of individuals at high risk of lung complications caused by environmental toxins and pollutants and (2) in clinical practice, in order to prevent the development of chronic bronchitis, which is a common disease.

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Glutathione S-transferase class ? in French alcoholic cirrhotic patients

Cited by 55 publications

References 19 publications

Gender difference in DNA adduct levels among nonsmoking lung cancer patients

Gender difference in DNA adduct levels among nonsmoking lung cancer patients

Aromatic DNA adducts in coke-oven workers, in relation to exposure, lifestyle and genetic polymorphism of metabolic enzymes

Peculiarities of the GSTM1 0/0 genotype in French heavy smokers with various types of chronic bronchitis

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